胶质瘤患者术后痫性发作及组织中高迁移率族蛋白B1的表达情况研究
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摘要
目的探究分析胶质瘤患者术后伴随癫痫的临床特征及组织中高迁移率族蛋白B1(high mobility group box protein B1,HMGB1)的表达情况。方法选择温州市人民医院2013年1月—2017年6月收治的101例胶质瘤术后痫性再发的患者,并选择同期该院收治的胶质瘤未发癫痫的患者70例、无肿瘤仅发癫痫的患者70例以及进行颅内减压手术的患者50例。比较胶质瘤并癫痫患者的临床病理因素对HMGB1表达的影响,比较4组患者的HMGB1表达情况,分析胶质瘤病理级别、癫痫的存在与否与胶质瘤组织中HMGB1表达的影响。结果胶质瘤并癫痫组患者致痫灶组织中HMGB1的表达与患者的性别、年龄和致痫灶所处位置差异无统计学意义(均P>0. 05);患者致痫灶组织中HMGB1的表达与患者的胶质瘤病理级别、癫痫持续时间和发作频率有明显相关性,差异具有统计学意义(均P <0. 05)。胶质瘤并癫痫组的HMGB1阳性表达量高于胶质瘤非癫痫组、无肿瘤癫痫组的HMGB1阳性表达量。对胶质瘤并癫痫组和胶质瘤非癫痫组不同病理级别的HMGB1的表达,低病理级别组中,胶质瘤并癫痫组患者的HMGB1表达量更高,差异有统计学意义(P <0. 05);高病理级别组中,胶质瘤并癫痫组患者的HMGB1表达量与胶质瘤非癫痫组相比,差异无统计学意义(P> 0. 05)。结论胶质瘤术后伴随痫性患者的胶质瘤组织中HMGB1表达量高,并与患者的病理级别、癫痫持续时间和发作频率有关,HMGB1的高表达可能与癫痫发作有关,具有临床研究价值。
Objective To investigate and analyze the clinical features of postoperative seizures of glioma patients and the expression of high mobility group box B1 protein in glioma tissues. Methods One hundred and one cases of glioma with recurrent epileptic seizure,70 cases of glioma without seizure,70 cases of epileptic seizure without glioma and 50 cases of cranial decompression in our hospital between January 2013 and June 2017 were enrolled into this study. The clinical pathological factors that affected the expression of HMGB in the glioma patients with epileptic seizure were explored. The expression of HMGB1 in the brain tissues were compared among the four groups. The effects of pathological grade of glioma and the accompanied with epilepsy on the expression of HMGB1 were analyzed. Results The expression of HMGB1 in epileptogenic focus tissues was not obviously related to the gender,age and location of the epileptogenic foci in the Glioma patients with epileptic seizure( all P > 0. 05),however,it significantly related to the pathological grade of glioma,episode time and frequency( all P < 0. 05). The positive expression of HMGB1 in the glioma group with epilepsy was higher than that in other three groups. In the patients with lower pathological grade glioma,the expression of HMGB1 in glioma patients with epileptic seizure was higher than that in the glioma patients without seizure,the difference was significant( P < 0. 05). In the patients with higher pathological grade glioma,there was no significant difference in the expression of HMGB1 between glioma patients with epileptic seizure and without seizure( P > 0. 05). Conclusion The expression of HMGB1 in glioma patients with recurrent epileptic seizure is high,which is associated with the histological grade,episode time and seizure frequency. The high expression of HMGB1 may be related to epilepsy,its clinical research value should be paid.
Objective To investigate and analyze the clinical features of postoperative seizures of glioma patients and the expression of high mobility group box B1 protein in glioma tissues. Methods One hundred and one cases of glioma with recurrent epileptic seizure,70 cases of glioma without seizure,70 cases of epileptic seizure without glioma and 50 cases of cranial decompression in our hospital between January 2013 and June 2017 were enrolled into this study. The clinical pathological factors that affected the expression of HMGB in the glioma patients with epileptic seizure were explored. The expression of HMGB1 in the brain tissues were compared among the four groups. The effects of pathological grade of glioma and the accompanied with epilepsy on the expression of HMGB1 were analyzed. Results The expression of HMGB1 in epileptogenic focus tissues was not obviously related to the gender,age and location of the epileptogenic foci in the Glioma patients with epileptic seizure( all P > 0. 05),however,it significantly related to the pathological grade of glioma,episode time and frequency( all P < 0. 05). The positive expression of HMGB1 in the glioma group with epilepsy was higher than that in other three groups. In the patients with lower pathological grade glioma,the expression of HMGB1 in glioma patients with epileptic seizure was higher than that in the glioma patients without seizure,the difference was significant( P < 0. 05). In the patients with higher pathological grade glioma,there was no significant difference in the expression of HMGB1 between glioma patients with epileptic seizure and without seizure( P > 0. 05). Conclusion The expression of HMGB1 in glioma patients with recurrent epileptic seizure is high,which is associated with the histological grade,episode time and seizure frequency. The high expression of HMGB1 may be related to epilepsy,its clinical research value should be paid.
引文
[1]王新军,杨如意,周少龙,等.胶质瘤相关性癫痫致痫灶组织中高迁移率族蛋白B1的表达[J].郑州大学学报(医学版),2015,50(5):634-637.
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[3]周少龙,王新军,付旭东,等.伴有癫痫的人脑胶质瘤中HMGB1/TLR4的表达研究[J].中华神经医学杂志,2015,14(6):563-566.
[4]高翔,罗林.胶质瘤中MGMT、BRAF和EGFR的变化及其治疗的研究进展[J].肿瘤药学,2016,6(1):21-25.
[5]文志华,宋小兵,江普查,等.脑胶质瘤中凋亡抑制生存基因和细胞周期蛋白b1的表达及临床意义[J].检验医学与临床,2016,13(6):765-767.
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[8]高海滨,周剑云,王利清,等.人cyclin B1对神经胶质瘤细胞增殖、侵袭和转移的影响[J].山东医药,2016,56(40):43-44.
[9]殷实,马晓东,韩国庆,等.胶质瘤合并癫痫的诊疗体会[J].解放军医学院学报,2014,35(7):704-706.
[10]周海霞,韩亮,祝子峰,等. HOXB1与mi R-3175在人胶质瘤中的表达及临床意义[J].国际肿瘤学杂志,2016,43(11):817-821.
[11] MASCHIO M,DINAPOLI L,FABI A,et al. Cognitive rehabilitation training in patients with brain tumor-related epilepsy and cognitive deficits:a pilot study[J]. J Neurooncol,2015,125(2):419.
[12]王猛,姚丽芬. BRAF基因突变在胶质瘤中研究进展[J].中华实用诊断与治疗杂志,2016,30(12):1156-1158.
[13] HUANG J,HE Y,CHEN M,et al. Adenosine deaminase and adenosine kinase expression in human glioma and their correlation with glioma-associated epilepsy[J]. Mol Med Rep,2015,12(5):6509-6516.
[14]杨天权,吴庭枫,李炎炎,等. mi R-16通过调控NF-κB1/MMP-9信号通路抑制胶质瘤侵袭的实验研究[J].中华神经医学杂志,2014,13(11):1081-1087.
[15] ZHANG Z,ZHENG W,YINYAN W,et al. IDH1/2 mutation is associated with seizure as an initial symptom in low-grade glioma:A report of 311 Chinese adult glioma patients[J]. Epilepsy Res,2015,109(1):100-105.
[16] KANEKO Y,PAPPAS C,MALAPIRA T,et al. Extracellular HMGB1Modulates Glutamate Metabolism Associated with Kainic Acid-Induced Epilepsy-Like Hyperactivity in Primary Rat Neural Cells[J].Cell Physiol Biochem,2017,41(3):947.
[17]张元峰,孙瑞迅,董孟宁,等.人脑胶质瘤组织中高迁移率族蛋白B1的表达及其与病理分级的关系[J].中国老年学杂志,2016,36(18):4500-4501.
[18]范真,黄斌,孙红斌.高迁移率族蛋白1抑制剂甘草酸对大鼠癫痫持续状态后血脑屏障的影响[J].国际神经病学神经外科学杂志,2015,42(4):311-315.
[19]林岚,杨淑,沈云松,等.胶质瘤相关性癫痫组织高迁移率族蛋白B1的表达[J].中国老年学杂志,2016,36(23):5837-5839.
[20] HUANG J,CHEN M N,DU J,et al. Differential Expression of Adenosine P1 Receptor ADORA1 and ADORA2A Associated with Glioma Development and Tumor-Associated Epilepsy[J]. Neurochemical Res,2016,41(7):1-10.
[21] IUS T,PAULETTO G,ISOLA M,et al. Surgery for insular low-grade glioma:predictors of postoperative seizure outcome[J]. J Neuro,2014,120(1):12-23.
[2]王新军,杨如意,单峤,等.胶质瘤相关性癫痫瘤组织中高迁移率族蛋白B1表达与痫性发作[J].中国神经精神疾病杂志,2015,41(3):155-159.
[3]周少龙,王新军,付旭东,等.伴有癫痫的人脑胶质瘤中HMGB1/TLR4的表达研究[J].中华神经医学杂志,2015,14(6):563-566.
[4]高翔,罗林.胶质瘤中MGMT、BRAF和EGFR的变化及其治疗的研究进展[J].肿瘤药学,2016,6(1):21-25.
[5]文志华,宋小兵,江普查,等.脑胶质瘤中凋亡抑制生存基因和细胞周期蛋白b1的表达及临床意义[J].检验医学与临床,2016,13(6):765-767.
[6] MURATA Y,WATANABE O,TANIGUCHI G,et al. A case of autoimmune epilepsy associated with anti-leucine-rich glioma inactivated subunit 1 antibodies manifesting electrical shock-like sensations and transparent sadness[J]. Epilepsy Behav Case Rep,2015,4:91-93.
[7] AGHI M K,NAHED B V,SLOAN A E,et al. The role of surgery in the management of patients with diffuse low grade glioma:A systematic review and evidence-based clinical practice guideline[J]. J Neurooncol,2015,125(3):503-530.
[8]高海滨,周剑云,王利清,等.人cyclin B1对神经胶质瘤细胞增殖、侵袭和转移的影响[J].山东医药,2016,56(40):43-44.
[9]殷实,马晓东,韩国庆,等.胶质瘤合并癫痫的诊疗体会[J].解放军医学院学报,2014,35(7):704-706.
[10]周海霞,韩亮,祝子峰,等. HOXB1与mi R-3175在人胶质瘤中的表达及临床意义[J].国际肿瘤学杂志,2016,43(11):817-821.
[11] MASCHIO M,DINAPOLI L,FABI A,et al. Cognitive rehabilitation training in patients with brain tumor-related epilepsy and cognitive deficits:a pilot study[J]. J Neurooncol,2015,125(2):419.
[12]王猛,姚丽芬. BRAF基因突变在胶质瘤中研究进展[J].中华实用诊断与治疗杂志,2016,30(12):1156-1158.
[13] HUANG J,HE Y,CHEN M,et al. Adenosine deaminase and adenosine kinase expression in human glioma and their correlation with glioma-associated epilepsy[J]. Mol Med Rep,2015,12(5):6509-6516.
[14]杨天权,吴庭枫,李炎炎,等. mi R-16通过调控NF-κB1/MMP-9信号通路抑制胶质瘤侵袭的实验研究[J].中华神经医学杂志,2014,13(11):1081-1087.
[15] ZHANG Z,ZHENG W,YINYAN W,et al. IDH1/2 mutation is associated with seizure as an initial symptom in low-grade glioma:A report of 311 Chinese adult glioma patients[J]. Epilepsy Res,2015,109(1):100-105.
[16] KANEKO Y,PAPPAS C,MALAPIRA T,et al. Extracellular HMGB1Modulates Glutamate Metabolism Associated with Kainic Acid-Induced Epilepsy-Like Hyperactivity in Primary Rat Neural Cells[J].Cell Physiol Biochem,2017,41(3):947.
[17]张元峰,孙瑞迅,董孟宁,等.人脑胶质瘤组织中高迁移率族蛋白B1的表达及其与病理分级的关系[J].中国老年学杂志,2016,36(18):4500-4501.
[18]范真,黄斌,孙红斌.高迁移率族蛋白1抑制剂甘草酸对大鼠癫痫持续状态后血脑屏障的影响[J].国际神经病学神经外科学杂志,2015,42(4):311-315.
[19]林岚,杨淑,沈云松,等.胶质瘤相关性癫痫组织高迁移率族蛋白B1的表达[J].中国老年学杂志,2016,36(23):5837-5839.
[20] HUANG J,CHEN M N,DU J,et al. Differential Expression of Adenosine P1 Receptor ADORA1 and ADORA2A Associated with Glioma Development and Tumor-Associated Epilepsy[J]. Neurochemical Res,2016,41(7):1-10.
[21] IUS T,PAULETTO G,ISOLA M,et al. Surgery for insular low-grade glioma:predictors of postoperative seizure outcome[J]. J Neuro,2014,120(1):12-23.