针刺预处理不同配穴对延缓运动性疲劳及心脏功能调节的影响
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摘要
目的探讨针刺预处理不同配穴对延缓运动性疲劳及对心脏功能调节的影响。方法选用SD大鼠随机分为空白组、运动组、保健穴组、标本配穴组。除空白组外,每组进行6周的递增负荷训练。保健穴组和标本配穴组每次运动前1h进行针刺预处理,共治疗6周。采用ELISA法检测心钠素(ANP),全自动生化仪检测乳酸(LD)、肌酸激酶(CK)、尿素氮(BUN)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSHPX)、超氧化物岐化酶(T-SOD)。结果与空白组相比,运动组血清LD、CK、BUN含量均明显增加(<0.05);与运动组相比,保健穴组、标本配穴组血清LD、CK、BUN含量均明显降低(<0.05);标本配穴组优于保健穴组(<0.05)。与空白组相比,运动组大鼠心肌组织ANP、MDA含量明显增加(<0.05),TSOD、GSH-PX含量明显降低(<0.05);与运动组相比,保健穴组、标本配穴组心肌组织ANP、MDA含量均明显降低(<0.05),T-SOD、GSH-PX含量均明显增加(<0.05);而标本配穴组效果优于保健穴组(<0.05)。结论针刺预处理可以缓解运动性疲劳的产生,提高运动能力;对运动过程中的心肌组织具有抗氧化作用并能调节心脏的内分泌功能,且标本配穴优于保健穴配伍。
Objective To investigate the effects of acupuncture preconditioning with different prescriptions of acupoints on delaying exercise-induced fatigue and regulating cardiac function. Methods SD rats were randomly divided into blank group,exercise group,health-care acupoint group,and acupoint-matching group. In addition to blank group,each group carried out 6 weeks of incremental load training.Health-care acupoint group and acupoint-matching group were treated with acupuncture pretreatment for 6 weeks before each exercise. The ANP was detected by Elisa,and the Lactic acid(LD),creatine kinase(CK),urea nitrogen(BLU),malondialdehyde(MDA),glutathione peroxidase(gsh-px)and superoxide dismutase(T-SOD)were detected by automatic biochemical analyzer.Compared to blank group,the contents of LD,CK and Bun in exercise group were obviously increased( <0.05),and the contents of LD,CK and Bun in health-care acupoint group and acupoint-matching group were significantly decreased( <0.05)compared with those in exercise group( <0.01).Compared with blank group,the contents of ANP and MDA increased significantly( <0.05),and the T-sod and gsh-px contents of myocardium were significantly decreased( <0.05).Compared with exercise group,the contents of ANP and MDA in myocardium of health-care acupoint group and acupoint matching group were significantly decreased( <0.05),the contents of T-sod and gsh-px in myocardium were obviously increased( <0.05),while that of acupoint-matching group was better than that of health-care acupoint group( <0.01).Conclusion(1)The acupuncture pretreatment can relieve the athletic fatigue and improve the athletic ability,and the efficacy of acupointmatching method is more obvious;(2)Acupuncture pretreatment has antioxidant effect on myocardium in the course of exercise and can regulate the endocrine function of the heart,and the points of acupoint-matching are superior to the compatibility of health-care acupoint.
Objective To investigate the effects of acupuncture preconditioning with different prescriptions of acupoints on delaying exercise-induced fatigue and regulating cardiac function. Methods SD rats were randomly divided into blank group,exercise group,health-care acupoint group,and acupoint-matching group. In addition to blank group,each group carried out 6 weeks of incremental load training.Health-care acupoint group and acupoint-matching group were treated with acupuncture pretreatment for 6 weeks before each exercise. The ANP was detected by Elisa,and the Lactic acid(LD),creatine kinase(CK),urea nitrogen(BLU),malondialdehyde(MDA),glutathione peroxidase(gsh-px)and superoxide dismutase(T-SOD)were detected by automatic biochemical analyzer.Compared to blank group,the contents of LD,CK and Bun in exercise group were obviously increased( <0.05),and the contents of LD,CK and Bun in health-care acupoint group and acupoint-matching group were significantly decreased( <0.05)compared with those in exercise group( <0.01).Compared with blank group,the contents of ANP and MDA increased significantly( <0.05),and the T-sod and gsh-px contents of myocardium were significantly decreased( <0.05).Compared with exercise group,the contents of ANP and MDA in myocardium of health-care acupoint group and acupoint matching group were significantly decreased( <0.05),the contents of T-sod and gsh-px in myocardium were obviously increased( <0.05),while that of acupoint-matching group was better than that of health-care acupoint group( <0.01).Conclusion(1)The acupuncture pretreatment can relieve the athletic fatigue and improve the athletic ability,and the efficacy of acupointmatching method is more obvious;(2)Acupuncture pretreatment has antioxidant effect on myocardium in the course of exercise and can regulate the endocrine function of the heart,and the points of acupoint-matching are superior to the compatibility of health-care acupoint.
引文
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[6]卢继东,吴松,刘建民,等.电针对心肌缺血大鼠细胞凋亡指数及mi RNAs表达影响的研究[J].中华中医药学刊,2016,34(7):1599-1602.
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[8]王华,望庐山,梁凤霞,等.电针治疗对慢性心肌缺血大鼠心电图、心肌病理形态及PI3K/Akt信号通路的影响[J].中国针灸,2016,36(4):389-395.
[9]王华,望庐山,梁凤霞,等.基于PI3K/AKT信号通路探讨标本配穴电针对慢性心肌缺血大鼠的保护作用及机制[J].中华中医药杂志,2016,31(9):3475-3480.
[10]李丽,常波.运动人体科学过度训练动物模型制备的研究进展[J].沈阳体育学院学报,2011,30(5):77-82.
[11]华兴邦,李辞蓉,周浩良,等.大鼠穴位图谱的研制[J].实验动物和动物实验,1991(1):1-5.
[12]陈泽斌,王华.大鼠穴位针刺预处理抗脑缺血再灌注损伤作用探讨[J].针刺研究,2002,28(3):165-169.
[13]李虹霖,王玉珏,夏昆鹏,等.不同干预手法作用于足三里穴对运动性疲劳大鼠脑内5-HT的影响[J].针灸临床杂志,2015,31(8):71-72.
[14]华冰.电针内关穴预处理对力竭运动大鼠心肌线粒体NO、游离Ca2+和ATP含量的影响[J].台州学院学报,2012,34(6):44-47.
[15]谭成富,严洁,王超,等.谢文娟针灸预处理对心肌缺血再灌注损伤兔不同时间热休克蛋白27、70、90表达的影响[J].针刺研究,2017,42(1):31-38.
[16]陈晨,孙忠人,吕晓琳,等.针灸预处理改善心肌缺血再灌注损伤机制的研究[J].针灸临床杂志,2016,32(11):102-106.
[17]常芸,林福美.不同类型运动心脏的内分泌改变特征[J].中国运动医学杂志,1996(3):162-169.
[18]顾一煌,任建宁,刘瑜,等.艾灸对力竭运动小鼠心肌细胞内分泌功能的影响[J].南京中医药大学学报,2012,28(1):71-73.
[19]顾长海.艾灸对运动训练大鼠血乳酸、心钠素、皮质醇及脏器指数水平影响的实验研究[J].山东体育学院学报,2007,23(4):67-69.
[2]舒晴,孙丹红,王华,等.针刺与艾灸对气虚证心率变异性的不同影响:随机对照研究[J].中国针灸,2017,37(1):25-30.
[3]刘建民,吕景芳,王华,等.“标本配穴”电针法对老年阳虚证模型大鼠肝脏能量代谢的影响[J].湖北中医药大学学报,2016,18(5):1-4.
[4]刘建民,吕景芳,王华,等.“双固一通”电针法对老年阳虚证模型大鼠肝线粒体结构及ATP酶活性的影响[J].上海针灸杂志,2015,34(1):63-66.
[5]王华,刘静,刘建民,等.“双固一通”电针对老年阳虚大鼠抗疲劳能力及肝脏线粒体呼吸功能的影响[J].针刺研究,2013,38(4):259-264.
[6]卢继东,吴松,刘建民,等.电针对心肌缺血大鼠细胞凋亡指数及mi RNAs表达影响的研究[J].中华中医药学刊,2016,34(7):1599-1602.
[7]谢俊,王华,吴松,等.标本配穴对心肌缺血大鼠心肌沉默信息调节因子1的影响[J].中华中医药杂志,2016,31(7):2761-2764.
[8]王华,望庐山,梁凤霞,等.电针治疗对慢性心肌缺血大鼠心电图、心肌病理形态及PI3K/Akt信号通路的影响[J].中国针灸,2016,36(4):389-395.
[9]王华,望庐山,梁凤霞,等.基于PI3K/AKT信号通路探讨标本配穴电针对慢性心肌缺血大鼠的保护作用及机制[J].中华中医药杂志,2016,31(9):3475-3480.
[10]李丽,常波.运动人体科学过度训练动物模型制备的研究进展[J].沈阳体育学院学报,2011,30(5):77-82.
[11]华兴邦,李辞蓉,周浩良,等.大鼠穴位图谱的研制[J].实验动物和动物实验,1991(1):1-5.
[12]陈泽斌,王华.大鼠穴位针刺预处理抗脑缺血再灌注损伤作用探讨[J].针刺研究,2002,28(3):165-169.
[13]李虹霖,王玉珏,夏昆鹏,等.不同干预手法作用于足三里穴对运动性疲劳大鼠脑内5-HT的影响[J].针灸临床杂志,2015,31(8):71-72.
[14]华冰.电针内关穴预处理对力竭运动大鼠心肌线粒体NO、游离Ca2+和ATP含量的影响[J].台州学院学报,2012,34(6):44-47.
[15]谭成富,严洁,王超,等.谢文娟针灸预处理对心肌缺血再灌注损伤兔不同时间热休克蛋白27、70、90表达的影响[J].针刺研究,2017,42(1):31-38.
[16]陈晨,孙忠人,吕晓琳,等.针灸预处理改善心肌缺血再灌注损伤机制的研究[J].针灸临床杂志,2016,32(11):102-106.
[17]常芸,林福美.不同类型运动心脏的内分泌改变特征[J].中国运动医学杂志,1996(3):162-169.
[18]顾一煌,任建宁,刘瑜,等.艾灸对力竭运动小鼠心肌细胞内分泌功能的影响[J].南京中医药大学学报,2012,28(1):71-73.
[19]顾长海.艾灸对运动训练大鼠血乳酸、心钠素、皮质醇及脏器指数水平影响的实验研究[J].山东体育学院学报,2007,23(4):67-69.