极低频电磁暴露对钙离子跨膜迁移的影响及机理分析
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摘要
目的:研究极低频(ELF)电磁暴露对细胞钙离子跨膜迁移的影响。方法:以人体肝癌细胞为对象,激光扫描共聚焦显微镜为检测手段,研究细胞钙离子跨膜迁移对ELF电磁暴露的响应。结果:在磁场强度为1.78×10-7T条件下,与对照组对比,各暴露组均对不同频率和电场强度组合的外加电磁暴露环境有响应,其中f=16 Hz且EP=53 V m、f=45 Hz且EP=53 V m以及f=16 Hz且EP=80 V m的电磁暴露可使钙离子跨膜迁移量显著上升,而f=32 Hz且EP=53 V m、f=60 Hz且EP=53 V m、f=16 Hz且EP=26 V m以及f=16 Hz且EP=87 V m的电磁暴露未使钙离子跨膜迁移量显著上升。结论:不同频率和电场强度组合的ELF电磁暴露均会引起钙离子跨膜迁移量产生变化且呈现出明显差异,这可为ELF电磁环境下生物学应用提供实验依据;注重细胞膜离子通道物理、生物特性基础上探讨ELF电磁暴露对钙离子跨膜迁移影响的机理。
Objective To study the effects of extremely low frequency(ELF) electromagnetic exposure on calcium ion transmembrane migration. Methods The human hepatoma cells were taken as the subjects in this study. The responses of calcium ion transmembrane migration to ELF electromagnetic exposure were detected by laser scanning confocal microscopy. Results Under the condition that the magnetic field strength remained at 1.78×10-7T, the transmembrane migration of calcium ion in exposure groups was responded to the applied electromagnetic exposure environment with different properties. With the frequency(f) of 16 Hz or 45 Hz and electric field peak(EP) of 53 V/m, f of 16 Hz and EPof 80 V/m, the ELF electromagnetic exposure made calcium ion transmembrane migration increased significantly, while calcium ion transmembrane migration wasn't increased significantly under the exposure with f of 32 Hz or 60 Hz and EPof 53 V/m, f of 16 Hz and EPof 26 V/m or 87 V/m. Conclusion The combination of different frequencies and electric field peaks of ELF electromagnetic exposure can increase calcium ion transmembrane migration, and the differences are significant, which provides experimental basis for the biological application of ELF electromagnetic exposure. The mechanism of the effects of ELF electromagnetic exposure on calcium ion transmembrane migration should be discussed based on the physical and biological characteristics of cell membrane ion channels.
Objective To study the effects of extremely low frequency(ELF) electromagnetic exposure on calcium ion transmembrane migration. Methods The human hepatoma cells were taken as the subjects in this study. The responses of calcium ion transmembrane migration to ELF electromagnetic exposure were detected by laser scanning confocal microscopy. Results Under the condition that the magnetic field strength remained at 1.78×10-7T, the transmembrane migration of calcium ion in exposure groups was responded to the applied electromagnetic exposure environment with different properties. With the frequency(f) of 16 Hz or 45 Hz and electric field peak(EP) of 53 V/m, f of 16 Hz and EPof 80 V/m, the ELF electromagnetic exposure made calcium ion transmembrane migration increased significantly, while calcium ion transmembrane migration wasn't increased significantly under the exposure with f of 32 Hz or 60 Hz and EPof 53 V/m, f of 16 Hz and EPof 26 V/m or 87 V/m. Conclusion The combination of different frequencies and electric field peaks of ELF electromagnetic exposure can increase calcium ion transmembrane migration, and the differences are significant, which provides experimental basis for the biological application of ELF electromagnetic exposure. The mechanism of the effects of ELF electromagnetic exposure on calcium ion transmembrane migration should be discussed based on the physical and biological characteristics of cell membrane ion channels.
引文
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[15]刘银春.木材对5.43μm红外线的吸收及其穿透规律的研究[J].福建林学院学报,2002,22(3):222-231.LIU Y C.Study on the law of experiment of wood absorbed 5.43μm infrared and it's penetrability for the infrared[J].Journal of Fujian College of Forestry,2002,22(3):222-231.
[16]ZHOU J L,LI C L,YAO G D,et al.Gene expression of cytokine receptore in HL60 cells exposed to a 50 Hz magnetic field[J].Bioelectromagnetics,2002,23(5):339-346.
[17]张绘蓝.全息空间光孤子的理论研究[D].武汉:华中科技大学,2007:3-10.ZHANG H L.Theoretical study on holographic optical solitons[D].Wuhan:Huazhong University of Science and Technology,2007:3-10.
[2]高攸纲,刁庆安.再谈工频电磁场对人体健康的危害影响[C].成都:全国第二届毫米波医学应用及第一届电磁辐射生物学学术研讨会论文汇编,2001:4-6.GAO Y G,DIAO Q A.Further discussion on the impact of power frequency electromagnetic field on human health[C].Chengdu:Compilation of the Second National Millimeter Wave Madical Application and the First Academic Conference on Electromagnetic Radiation Biology,2001:4-6.
[3]WERTHEIMER N,LEEPER E.Electrical wiring configurations and childhood cancer[J].Am J Epedermiol,1979,109:273-284.
[4]刘欣,赵龙宇,陈慧玲,等.极低频电磁场暴露对从业人员肝脏功能的影响[J].中华劳动卫生职业病杂志,2013,31(8):599-601.LIU X,ZHAO L Y,CHEN H L,et al.Effect of exposure to extremely low-frequency electromagnetic fields on liver function of workers[J].Chinese Journal of Industrial Hygiene and Occupational Diseases,2013,31(8):599-601.
[5]GRELLIER J,RAVAZZANI P,CARDIS E.Potential health impacts of residential exposures to extremely low frequency magnetic fields in Europe[J].Environ Int,2014,62:55-63.
[6]曲敏,毕卓悦,唐雨萌,等.生物电磁技术应用及其相关健康风险评估对策[J].高电压技术,2015,41(8):2625-2634.QU M,BI Z Y,TANG Y M,et al.Application of bio-electromagnetic technique and its related health risk assessment strategy[J].High Voltage Engineering,2015,41(8):2625-2634.
[7]田冰,贾彩丽,夏若红,等.50 Hz脉冲电场作用下胰岛素构象变化的拉曼光谱分析[J].光谱与光谱分析,2004,24(11):1334-1337.TIAN B,JIA C L,XIA R H,et al.Raman analysis of conformation changes of insulin solvent after being exposed to ELF pulsed electric field[J].Spectroscopy and Spectral Analysis,2004,24(11):1334-1337.
[8]LINSTROM E,LINSTROM P,BERGLUND A,et al.Intracellular calcium oscillations induced in a T-cell line by a weak 50 Hz magnetic field[J].Cell Physiol,1993,156(2):395-398.
[9]BARBIER E,DUFY B,VEYRET B.Stimulation of Ca2+influx in rat pituitary cells under exposure to a 50 Hz magnetic field[J].Bioelectromagnetics,1996,17(4):303-311.
[10]柴文祥,李彤,梁继河,等.重复急性缺氧再给氧对培养鼠主动脉内皮细胞内钙的影响[J].第四军医大学学报,1997,18(12):43-45.CHAI W X,LI T,LIANG J H,et al.The effect of repeated hypoxia and reoxygenation on calcium in cultured rat aortic endothelial cells[J].Journal of the Fourth Military Medical University,1997,18(12):43-45.
[11]王保义,唐敬贤,江汉保.电磁场在生物医学中的应用[M].北京:国防工业出版社,1990:80-135.WANG B Y,TANG J X,JIANG H B.Application of electromagnetic field in biomedicine[M].Beijing:National Defense Industry Press,1990:80-135.
[12]牛中奇.电磁波的生物窗效应及其回旋谐振理论[J].中国生物医学工程学报,1993,12(1):61-69.NIU Z Q.Biological window effects of electromagnetic-wave and the cyclotronresonance theory[J].Chinese Journal of Biomedical Engineering,1993,12(1):61-69.
[13]郝柏林.物理学和生物学[J].物理,2003,32(6):353-359.HAO B L.Physics and biology[J].Physics,2003,32(6):353-359.
[14]刘银春,尤华明.喷浇磁水的灵芝细胞超微结构的变化[J].福建林学院学报,2001,21(3):220-223.LIU Y C,YOU H M.Cell ultrastructure of canoderma lacidum sprinkled with magnetized water[J].Journal of Fujian College of Forestry,2001,21(3):220-223.
[15]刘银春.木材对5.43μm红外线的吸收及其穿透规律的研究[J].福建林学院学报,2002,22(3):222-231.LIU Y C.Study on the law of experiment of wood absorbed 5.43μm infrared and it's penetrability for the infrared[J].Journal of Fujian College of Forestry,2002,22(3):222-231.
[16]ZHOU J L,LI C L,YAO G D,et al.Gene expression of cytokine receptore in HL60 cells exposed to a 50 Hz magnetic field[J].Bioelectromagnetics,2002,23(5):339-346.
[17]张绘蓝.全息空间光孤子的理论研究[D].武汉:华中科技大学,2007:3-10.ZHANG H L.Theoretical study on holographic optical solitons[D].Wuhan:Huazhong University of Science and Technology,2007:3-10.