人参皂甙Rh2对人胶质瘤细胞内钙离子浓度的影响
|
摘要
目的观察人参皂甙Rh2对人胶质瘤细胞内钙离子浓度的影响。方法实验分为对照组和人参皂甙Rh2组,对照组应用常规培养基培养人胶质瘤细胞株U87MG,人参皂甙Rh2组在常规培养基中人参皂甙Rh2的浓度为20μg/m L,利用激光共聚焦显微镜和流式细胞仪检测各组培养的人胶质瘤细胞内钙离子浓度,再利用Western blot技术检测人胶质瘤细胞Cav1.2蛋白的表达,最后利用膜片钳技术检测人胶质瘤细胞L型电压依赖型钙离子通道的功能。结果人参皂甙Rh2处理U87MG细胞后,细胞内钙离子浓度是对照组的2.5倍(P<0. 05);人参皂甙Rh2培养后胶质瘤细胞Cav1.2蛋白表达量是对照组的1.5倍(P<0. 05),且L型电压依赖性钙离子通道功能增强。结论人参皂甙Rh2可通过增加胶质瘤细胞Cav1.2表达和L型电压依赖性钙离子通道的功能促进细胞内游离钙离子浓度的增加,从而诱导人胶质瘤细胞的凋亡。
Objective To investigate the effect of ginsenoside Rh2 on intracellular calcium concentration of human brain glioma cells and its mechanism. Methods Human U87 MG brain glioma cells were cultured in conventional medium( control group)and in conventional medium with 20 μg/m L ginsenoside Rh2( experimental group). We tested the intracellular calcium concentration of human brain glioma cells by laser scanning confocal microscope and flow cytometry. The expression of Cav1.2 was measured by the Western blotting. And the function of L-type calcium channel was measured by patch clamp. Results The treatment of ginsenoside Rh2 increased the intracellular calcium concentration of human brain glioma cells,promoted the expression of Cav1.2 and facilitated the function of L-type calcium channel. Conclusion Ginsenoside Rh2 increases the intracellular calcium concentration of human brain glioma cells by promoting the expression of Cav1.2 and facilitating the function of L-type calcium channel.
Objective To investigate the effect of ginsenoside Rh2 on intracellular calcium concentration of human brain glioma cells and its mechanism. Methods Human U87 MG brain glioma cells were cultured in conventional medium( control group)and in conventional medium with 20 μg/m L ginsenoside Rh2( experimental group). We tested the intracellular calcium concentration of human brain glioma cells by laser scanning confocal microscope and flow cytometry. The expression of Cav1.2 was measured by the Western blotting. And the function of L-type calcium channel was measured by patch clamp. Results The treatment of ginsenoside Rh2 increased the intracellular calcium concentration of human brain glioma cells,promoted the expression of Cav1.2 and facilitated the function of L-type calcium channel. Conclusion Ginsenoside Rh2 increases the intracellular calcium concentration of human brain glioma cells by promoting the expression of Cav1.2 and facilitating the function of L-type calcium channel.
引文
[1]Jue TR,Sena ES,Macleod MR,et al.A systematic review and meta-analysis of topoisomerase inhibition in pre-clinical glioma models[J].Oncotarget,2018,9(13):11387-11401.
[2]Tsymbal DO,Minchenko DO,Kryvdiuk IV,et al.Expression of proliferation related transcription factor genes in U87 glioma cells with IRE1 knockdown:upon glucose and glutamine deprivation[J].Fiziol Zh,2016,62(1):3-15.
[3]程光,章翔,鲍炜,等.RNA干扰基因敲除MAGE-1在恶性胶质瘤U87细胞中的初步研究[J].医学研究生学报,2006,4(2):292-297.
[4]吴彩云,张春妮.MicroRNA与神经胶质瘤关系的研究进展[J].医学研究生学报,2014,7(3):746-750.
[5]Moschovos C,Papatheodoropoulos C.The L-type voltage-dependent calcium channel long-term potentiation is higher in the dorsal compared with the ventral associational/commissural CA3 hippocampal synapses[J].Neurosci Res,2016,106(2):62-65.
[6]Sun Z,Cao X,Zhang Z,et al.Simulated microgravity inhibits L-type calcium channel currents partially by the up-regulation of miR-103 in MC3T3-E1 osteoblasts[J].Scientific Reports,2015,5(3):8077.
[7]姚兴军,洪新雨,刘兴吉,等.人参皂甙Rh2对脑胶质细胞瘤侵袭性的影响[J].中国实验诊断学,2005,21(6):36-39.
[8]权恺,刘群,李萍,等.人参皂苷抗癌活性的最新研究进展[J].医学研究生学报,2015,4(7):427-431.
[9]Shen F,Guo Q,Hu Q,et al.RelB,a good prognosis predictor,links cell-cycle and migration to glioma tumorigenesis[J].Oncol Lett,2018,15(4):4404-4410.
[10]Thust SC,Heiland S,Falini A,et al.Glioma imaging in Europe:A survey of 220 centres and recommendations for best clinical practice[J].Eur Radiol,2018,28(8):3306-3317.
[11]江山,陈莺,房德芳.人参皂甙Rh2抗海马脑片H2O2损伤作用及与GABAA受体的关系[J].中药药理与临床,2016,6(2):38-42.
[12]Park JA,Kim KW,Kim SI.Caspase specifically cleaves p21WAF1/CIP1 in the earlier stage of apotosis in SK-HEP-1 human hepatoma cells[J].Eur J Biochem,1998,257(1):242.
[13]Zhu P,Hu S,Jin Q,et al.Ripk3 promotes ER stress-induced necroptosis in cardiac IR injury:A mechanism involvingcalcium overload/XO/ROS/m PTP pathway[J].Redox Biol,2018,16(9):157-168.
[14]Santulli G,Lewis D,des Georges A,et al.Ryanodine Receptor Structure and Function in Health and Disease[J].Subcell Biochem,2018,87(4):329-352.
[15]Kolb I,Stoy WA,Rousseau EB.Cleaning patch-clamp pipettes for immediate reuse[J].Sci Rep,2016,11(3):35001.
[2]Tsymbal DO,Minchenko DO,Kryvdiuk IV,et al.Expression of proliferation related transcription factor genes in U87 glioma cells with IRE1 knockdown:upon glucose and glutamine deprivation[J].Fiziol Zh,2016,62(1):3-15.
[3]程光,章翔,鲍炜,等.RNA干扰基因敲除MAGE-1在恶性胶质瘤U87细胞中的初步研究[J].医学研究生学报,2006,4(2):292-297.
[4]吴彩云,张春妮.MicroRNA与神经胶质瘤关系的研究进展[J].医学研究生学报,2014,7(3):746-750.
[5]Moschovos C,Papatheodoropoulos C.The L-type voltage-dependent calcium channel long-term potentiation is higher in the dorsal compared with the ventral associational/commissural CA3 hippocampal synapses[J].Neurosci Res,2016,106(2):62-65.
[6]Sun Z,Cao X,Zhang Z,et al.Simulated microgravity inhibits L-type calcium channel currents partially by the up-regulation of miR-103 in MC3T3-E1 osteoblasts[J].Scientific Reports,2015,5(3):8077.
[7]姚兴军,洪新雨,刘兴吉,等.人参皂甙Rh2对脑胶质细胞瘤侵袭性的影响[J].中国实验诊断学,2005,21(6):36-39.
[8]权恺,刘群,李萍,等.人参皂苷抗癌活性的最新研究进展[J].医学研究生学报,2015,4(7):427-431.
[9]Shen F,Guo Q,Hu Q,et al.RelB,a good prognosis predictor,links cell-cycle and migration to glioma tumorigenesis[J].Oncol Lett,2018,15(4):4404-4410.
[10]Thust SC,Heiland S,Falini A,et al.Glioma imaging in Europe:A survey of 220 centres and recommendations for best clinical practice[J].Eur Radiol,2018,28(8):3306-3317.
[11]江山,陈莺,房德芳.人参皂甙Rh2抗海马脑片H2O2损伤作用及与GABAA受体的关系[J].中药药理与临床,2016,6(2):38-42.
[12]Park JA,Kim KW,Kim SI.Caspase specifically cleaves p21WAF1/CIP1 in the earlier stage of apotosis in SK-HEP-1 human hepatoma cells[J].Eur J Biochem,1998,257(1):242.
[13]Zhu P,Hu S,Jin Q,et al.Ripk3 promotes ER stress-induced necroptosis in cardiac IR injury:A mechanism involvingcalcium overload/XO/ROS/m PTP pathway[J].Redox Biol,2018,16(9):157-168.
[14]Santulli G,Lewis D,des Georges A,et al.Ryanodine Receptor Structure and Function in Health and Disease[J].Subcell Biochem,2018,87(4):329-352.
[15]Kolb I,Stoy WA,Rousseau EB.Cleaning patch-clamp pipettes for immediate reuse[J].Sci Rep,2016,11(3):35001.