HPLC法测定香苓开胃方中广藿香酮含量
|
摘要
目的:建立香苓开胃方中广藿香酮含量的HPLC测定方法。方法:采用高效液相色谱法(HPLC)测定香苓开胃方水煎剂中广藿香酮的含量,色谱柱Extend-C_(18)(4.6mm×250mm,5μm),流动相为乙腈(A)-0.2%的磷酸水溶液(B),梯度洗脱(0～20min,A 55%～60%),进样量:10μL,流速:1mL·min~(-1)。检测波长为310nm,柱温为30℃。结果:广藿香酮在0.00798～0.66500μg(r=1.0000)范围内线性关系良好;平均回收率(N=6)为108.58%,RSD为3.24%。香苓开胃方中广藿香酮含量范围为0.339～1.408 mg·dose~(-1)。结论:本实验方法简便准确,回收率、重复性均能达到要求,可用于香苓开胃方的质量控制。
Object To establish a method for determination of patchoulenone in Xiang Ling appetizing prescription by High Performance Liquid Chromatography(HPLC). Methods: The content of Patchoulenone from the water extract of Xiang Ling appetizing prescription was detected by HPLC. The Extend-C_(18)(4.6 mm×250 mm,5μm)column was used for chromatography. The mobile phase consisted of acetonitrile(A)-0.2%phosphoric(B) with solution gradient elute(0～20 min,A 55%～60%), injection volume was 10 μL, flow velocity was 1.0 mL·min~(-1), wave length was 310 nm,column temperature was set at 30 ℃. Results: In the range of 0.00798～0.66500μg(r=1.0000), Patchoulenone has a good linear regression, and average recovery was 108.58% with RSD3.24%(N =6). Purity of Patchoulenonein Xiang Ling appetizing prescription is 0.339～1.408 mg·dose~(-1). Conclusion: This method is simple and accurate, the recovery rate and repeatability can meet the requirements, and can be used for the quality control of Xiang Ling appetizing prescription.
Object To establish a method for determination of patchoulenone in Xiang Ling appetizing prescription by High Performance Liquid Chromatography(HPLC). Methods: The content of Patchoulenone from the water extract of Xiang Ling appetizing prescription was detected by HPLC. The Extend-C_(18)(4.6 mm×250 mm,5μm)column was used for chromatography. The mobile phase consisted of acetonitrile(A)-0.2%phosphoric(B) with solution gradient elute(0～20 min,A 55%～60%), injection volume was 10 μL, flow velocity was 1.0 mL·min~(-1), wave length was 310 nm,column temperature was set at 30 ℃. Results: In the range of 0.00798～0.66500μg(r=1.0000), Patchoulenone has a good linear regression, and average recovery was 108.58% with RSD3.24%(N =6). Purity of Patchoulenonein Xiang Ling appetizing prescription is 0.339～1.408 mg·dose~(-1). Conclusion: This method is simple and accurate, the recovery rate and repeatability can meet the requirements, and can be used for the quality control of Xiang Ling appetizing prescription.
引文
[1] Thomas Cascales. Infantile anorexia,co-excitation and co-mastery in the parent/baby cathexis:The contribution of Sigmund and Anna Freud[J]. The International Journal of Psychoanalysis,2017,98(2):393-414.
[2]陈珊红,周盈.小儿厌食症的中西医研究进展[J].新疆中医药,2018,36(01):117-120.
[3]宋辰斐,潘燕君,夏以琳.小儿厌食症的中西医研究进展[J].河北中医,2014,36(10):1580-1582.
[4]李其固,李振化,赵振宇,等. HPLC法测定抗病毒栓中的鸟苷(R,S)告依春,百秋里醇和广藿香酮[J].中成药,2014,36(06):1208-1212.
[5]甄亚钦,田伟,王迎春,等. HPLC波长切换法同时测定广藿香配方颗粒中咖啡酸、毛蕊花糖苷、异毛蕊花糖苷和广藿香酮的含量[J].中国实验方剂学杂志,2017,23(11):79-83.
[6]潘玄玄,宋粉云,李华,等. HPLC波长切换法同时测定沉香化气丸中7个成分的含量[J].药物分析杂志,2017,37(10):1832-1838.
[7]马丹凤,谭金桃,何周康.变换波长HPLC法同时测定藿香鼻炎合剂中五种成分的含量[J].实用药物与临床,2017,20(07):826-828.
[8]潘玄玄.沉香化气丸的质量控制及物质基础研究[D].广州:广东药科大学,2017.
[9]田伟,甄亚钦,王迎春,等.广藿香配方颗粒的HPLC指纹图谱研究[J].解放军药学学报,2017,33(02):162-164+197.
[10]秦臻.中药广藿香研究概述[J].医药前沿,2017,7(34):330-331.
[11]毕丹,张水英,任晋,等.超高效液相色谱法同时测定广藿香中6个成分的含量[J].药物分析杂志,2018,38(08):1331-1336.
[12]国家药典委员会.中华人民共和国药典(第一部)[M].北京:中国医药科技出版社,2015:45.
[13]张仲敏,黎玉翠,彭绍忠,等.广藿香醇研究概述[J].中国中医药信息杂志,2012,19(01):110-112.
[14]唐正伟.广藿香酮及其衍生物的合成与抗菌机理研究[D].成都:成都中医药大学,2016.
[2]陈珊红,周盈.小儿厌食症的中西医研究进展[J].新疆中医药,2018,36(01):117-120.
[3]宋辰斐,潘燕君,夏以琳.小儿厌食症的中西医研究进展[J].河北中医,2014,36(10):1580-1582.
[4]李其固,李振化,赵振宇,等. HPLC法测定抗病毒栓中的鸟苷(R,S)告依春,百秋里醇和广藿香酮[J].中成药,2014,36(06):1208-1212.
[5]甄亚钦,田伟,王迎春,等. HPLC波长切换法同时测定广藿香配方颗粒中咖啡酸、毛蕊花糖苷、异毛蕊花糖苷和广藿香酮的含量[J].中国实验方剂学杂志,2017,23(11):79-83.
[6]潘玄玄,宋粉云,李华,等. HPLC波长切换法同时测定沉香化气丸中7个成分的含量[J].药物分析杂志,2017,37(10):1832-1838.
[7]马丹凤,谭金桃,何周康.变换波长HPLC法同时测定藿香鼻炎合剂中五种成分的含量[J].实用药物与临床,2017,20(07):826-828.
[8]潘玄玄.沉香化气丸的质量控制及物质基础研究[D].广州:广东药科大学,2017.
[9]田伟,甄亚钦,王迎春,等.广藿香配方颗粒的HPLC指纹图谱研究[J].解放军药学学报,2017,33(02):162-164+197.
[10]秦臻.中药广藿香研究概述[J].医药前沿,2017,7(34):330-331.
[11]毕丹,张水英,任晋,等.超高效液相色谱法同时测定广藿香中6个成分的含量[J].药物分析杂志,2018,38(08):1331-1336.
[12]国家药典委员会.中华人民共和国药典(第一部)[M].北京:中国医药科技出版社,2015:45.
[13]张仲敏,黎玉翠,彭绍忠,等.广藿香醇研究概述[J].中国中医药信息杂志,2012,19(01):110-112.
[14]唐正伟.广藿香酮及其衍生物的合成与抗菌机理研究[D].成都:成都中医药大学,2016.