DNA-微阵列芯片法检验结核与非结核分枝杆菌的临床应用价值
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摘要
目的探讨DNA-微阵列芯片法检验结核与非结核分枝杆菌的临床应用价值。方法选择2016年12月—2018年3月该院痰结核培养或结核分杆菌痰涂片阳性标本108份。108份标本均采用改良罗氏培养法、DNA-微阵列芯片法检验,对二者检验结果不一致的标本进行测序检验,并将结果作为检验的金标准。评价改良罗氏法、DNA-微阵列芯片法对结核与非结核分枝杆菌的鉴别效果。结果改良罗氏培养法对结核分枝杆菌与非结核分枝杆菌检验的准确率为96.15%、97.56%,与DNA-微阵列芯片92.31%、96.34%对比差异无统计学意义(χ~2=0.253、0.265,P>0.05)。结论 DNA-微阵列芯片法在结核与非结核分枝杆菌检验中具有较高的准确率,且操作简便、快速,临床应用价值显著。
Objective To investigate the clinical value of DNA microarray in the detection of tuberculosis and non-tuberculous mycobacteria. Methods A total of 108 positive samples of sputum tuberculosis or tubercle bacillus sputum smear were collected from December 2016 to March 2018 in the hospital. All 108 specimens were tested by modified Roche culture method and DNA-microarray chip method. Sequencing tests were performed on specimens with inconsistent test results, and the results were used as the gold standard for testing. To evaluate the differential effect of modified Roche method and DNA-microarray chip method on tuberculosis and non-tuberculous mycobacteria. Results The accuracy of the modified Roche culture method for detecting mycobacterium tuberculosis and non-tuberculous mycobacteria was 96.15% and 97.56%. There was no significant difference between the modified Roche culture method and DNA-microarray chips 92.31% and 96.34% (χ~2=0.253, 0.265, P >0.05). Conclusion The DNA-microarray chip method has high accuracy in the detection of tuberculosis and non-tuberculous mycobacteria, and the operation is simple and rapid, and the clinical application value is significant.
Objective To investigate the clinical value of DNA microarray in the detection of tuberculosis and non-tuberculous mycobacteria. Methods A total of 108 positive samples of sputum tuberculosis or tubercle bacillus sputum smear were collected from December 2016 to March 2018 in the hospital. All 108 specimens were tested by modified Roche culture method and DNA-microarray chip method. Sequencing tests were performed on specimens with inconsistent test results, and the results were used as the gold standard for testing. To evaluate the differential effect of modified Roche method and DNA-microarray chip method on tuberculosis and non-tuberculous mycobacteria. Results The accuracy of the modified Roche culture method for detecting mycobacterium tuberculosis and non-tuberculous mycobacteria was 96.15% and 97.56%. There was no significant difference between the modified Roche culture method and DNA-microarray chips 92.31% and 96.34% (χ~2=0.253, 0.265, P >0.05). Conclusion The DNA-microarray chip method has high accuracy in the detection of tuberculosis and non-tuberculous mycobacteria, and the operation is simple and rapid, and the clinical application value is significant.
引文
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[2]赵静,王凤平,孙清清,等.DNA微阵列法检验结核分枝杆菌对利福平和异烟肼的耐药性[J].上海交通大学学报:医学版,2015,35(11):1651-1654.
[3]芮冬妹,朱珍,樊燕,等.基因芯片技术检验结核分枝杆菌对异烟肼耐药性的结果分析及临床价值[J].广西医学,2017,39(1):98-99.
[4]杨金华,弓艳娥.结核分枝杆菌特异性抗原γ-干扰素释放试验(IGRA)的临床应用价值分析[J].长治医学院学报,2016,30(4):301-303.
[5]钟业腾,林明冠,林翀,等.DNA微阵列芯片法检验结核分枝杆菌耐异烟肼基因的研究[J].中国热带医学,2017,17(3):270-273.
[6]沈彦恒,桂徐蔚,沈芸,等.T细胞斑点检验在鉴别结核分枝杆菌与非结核分枝杆菌感染中的价值[J].中国医刊,2016,51(4):78-80.
[7]张慧慧,熊国亮.SAT技术检验肺结核患者痰标本中结核分枝杆菌的临床应用研究[J].实验与检验医学,2015,33(4):407-409.
[8]汪金云,周东枝,徐亚丽.体外释放酶免疫技术检验结核分枝杆菌的临床应用[J].现代实用医学,2016,28(1):32-33.
[9]何建.DNA微阵列芯片法检测耐药结核分枝杆菌的价值探讨[J].传染病信息,2018,31(1):38-40.
[10]何莉,任微,梁雪妮,等.DNA芯片检测分枝杆菌的临床应用价值[J].中华临床医师杂志:电子版,2013,7(23):10611-10613.