甘氨酸补充对力竭性运动小鼠部分生化指标的影响
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摘要
目的探讨甘氨酸补充对一次力竭性运动小鼠部分生化指标的影响。方法将雄性昆明小鼠分为3组,每组10只:力竭性游泳运动组(E组),甘氨酸补充后力竭性游泳组(G组)及静息正常对照组(S组)。G组小鼠采用甘氨酸灌胃处理4周,其余两组给予生理盐水。4周后,E组和G组均做力竭性游泳运动。采样后进行补体溶血活性测定,并测定血清中部分生化指标的水平。结果与E组比较,G组的力竭性游泳运动时间显著延长(P<0.01)。力竭性游泳后,E组小鼠血清中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)、乳酸脱氢酶(LDH)、肌酸激酶(CK)、尿素氮、血清铁(Fe3+)和补体溶血活性(CHA)的水平较S组发生了显著性的变化(P<0.01),与E组比较,G组小鼠血清中上述生化指标得到了显著改善(P<0.01),其中ALP和LDH的水平可以恢复到与S组相当的水平(P>0.05)。结论力竭性游泳小鼠血清部分生化指标异常,甘氨酸预补充对这些异常有明显的改善作用,可以一定程度地提高小鼠的运动能力。
Objective To investigate the influence of glycine supplement on partial biochemical indicator in exhaustive exercise mice.Methods Divided Kunming′s male-mice into 3group(10/group):exhaustive swimming group(E),exhaustive swimming group with glycine supplement(G)and silent control group(S).Mice in the G group was treated with 4-weeked intragastric administration of glycine,while mice in other 2groups received physiological saline.After 4weeks,mice in E and G group did the exhaustive swimming exercise.After sampling,the activity of complement hemolytic activity was measured,and the serum levels of some biochemical indexes were determined.Results Time of exhaustive swimming in G group was significantly longer than E group(P<0.01).After exhaustive swimming,the ALT,AST,ALP,LDH,CK,N,Fe3+and CHA in E group were significantly changed compared with S group(P<0.01);while those indicators in G group were significantly improved than E group(P<0.01),the ALP and LDH level can regain to the level of S group(P>0.05).Conclusion Exhaustive swimming mice can have part indicator abnormality,but Glycine supplement can effectively improve that condition and enhance the exercise ability to some extent.
Objective To investigate the influence of glycine supplement on partial biochemical indicator in exhaustive exercise mice.Methods Divided Kunming′s male-mice into 3group(10/group):exhaustive swimming group(E),exhaustive swimming group with glycine supplement(G)and silent control group(S).Mice in the G group was treated with 4-weeked intragastric administration of glycine,while mice in other 2groups received physiological saline.After 4weeks,mice in E and G group did the exhaustive swimming exercise.After sampling,the activity of complement hemolytic activity was measured,and the serum levels of some biochemical indexes were determined.Results Time of exhaustive swimming in G group was significantly longer than E group(P<0.01).After exhaustive swimming,the ALT,AST,ALP,LDH,CK,N,Fe3+and CHA in E group were significantly changed compared with S group(P<0.01);while those indicators in G group were significantly improved than E group(P<0.01),the ALP and LDH level can regain to the level of S group(P>0.05).Conclusion Exhaustive swimming mice can have part indicator abnormality,but Glycine supplement can effectively improve that condition and enhance the exercise ability to some extent.
引文
[1]谷俊朝,马涛,王宇.甘氨酸保护作用机制与相关疾病探讨[J].北京医学,2005,27(9):560.
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[3]Barakat HA,Hamza AH.Glycine alleviates liver injury induced by deficiency in methionine and or choline in rats[J].Eur Rev Med Pharmacol Sci,2012,16(6):728-736.
[4]Senthilkumar R,Nalini N.Glycine prevents hepatic fibrosis by preventing the accumulation of collagen in rats with alcoholic liver injury[J].Pol J Pharmacol,2004,56(1):121-128.
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[6]Senthilkumar R,Nalini N.The potential beneficial effect of glycine on the carbohydrate moieties of glycoproteins in an experimental model of alcohol-induced hepatotoxicity[J].J Med Food,2004,7(1):108-113.
[7]钟孝正,陈琪.甘氨酸对心肌缺血再灌注损伤的保护作用及机制研究[J].中国病理生理杂志,2010,26(10):1953.
[8]秦霞,蒋莉,张咏梅,等.甘氨酸改善缺氧性MDCK细胞损伤的作用依赖于ERK1/2、Akt及p38MARK信号通路[J].南京医科大学学报,2012,32(10):1337-1342.
[9]谢剑梅,杨阁,李建,等.甘氨酸纳米脂质体的制备及其在脑缺血/再灌注中的神经保护作用研究[J].中国药理学通报,2012,28(9):1331-1332.
[10]王国华,姜正林,李霞,等.甘氨酸受体激动剂对大鼠局灶性脑缺血再灌注损伤的神经保护作用[J].临床神经病学杂志,2010,23(1):38-41.
[11]de Aguiar Picanco E,Lopes-Paulo F,Marques RG,et al.L-arginine and glycine supplementation in the repair of the irradiated colonic wall of rats[J].Int J Colorectal Dis,2011,26(5):561-568.
[12]Diestel CF,Marques RG,Lopes-Paulo F,et al.Role of Lglutamine and glycine supplementation on irradiated colonic wall[J].Int J Colorectal Dis,2007,22(12):1523-1529.
[13]武雯,刘近春,李俊华,等.甘氨酸对非酒精性脂肪性肝炎肠组织MIF表达的影响[J].中国病理生理杂志,2010,26(10):2029.
[14]吴惠文,韩德五,许瑞龄,等.甘氨酸对高果糖饮食诱导的大鼠胰岛素抵抗及认知功能损害的影响[J].中国病理生理杂志,2012,28(2):302-307.
[15]李腾,彭靖,吕尚军,等.甘氨酸对烧伤大鼠心肌细胞能量代谢的影响[J].重庆医学,2012,41(33):3476-3478.
[16]彭靖,李腾,吕尚军,等.甘氨酸减轻烧伤大鼠心肌损害的实验研究[J].重庆医学,2012,41(36):3804-3806.
[17]刘宏雁,吕国薇,王维忠.小鼠脑内甘氨酸水平在缺氧预适应中的变化[J].生理学报,2001,53(6):461-464.
[18]张东民,张佳民,马万云,等.急性力竭运动对大鼠下丘脑氨基酸神经递质的影响[J].高等学校化学学报,2002,23(2):230-233.
[19]甘露,黄庆军,张亚超,等.力竭性游泳对小鼠自主行为和免疫功能的影响[J].中国运动医学杂志,2004,23(4):434-435.
[20]武建国.实用临床免疫学检验[M].苏州:江苏科技出版社,1989.
[21]Zhang Y,Lv SJ,Yan H,et al.Effects of glycine supplementation on myocardial damage and cardiac function after severe burn[J].Burns,2013,39(4):729-735.
[22]周军利,黄跃生,党永明.甘氨酸对缺血缺氧心肌细胞能量代谢的影响[J].现代生物医学进展,2008,8(6):1093-1095.
[23]张水军,史冀华,唐哲.甘氨酸对脑死亡大鼠供肝损伤的防护作用[J].中华器官移植杂志,2004,25(5):264-266.
[24]秦杰,谢立平.氨基酸在肾功能保护中的作用研究进展[J].国外医学泌尿系统分册,2003,23(4):374-377.
[25]秦霞,曹剑秋,柏惠,等.甘氨酸对ATP耗竭诱生性细胞自噬的作用及其机制[J].南京医科大学学报,2009,29(12):1633-1637.
[26]Pan C,Bai X,Fan L,et al.Cytoprotection by glycine against ATP-depletion-induced injury is mediated by glycine receptor in renal cells[J].Biochem J,2005,390(Pt2):447-453.
[27]Perez-Torres I,Ibarra B,Soria-Castro E,et al.Effect of glycine on the cyclooxygenase pathway of the kidney arachidonic acid metabolism in a rat model of metabolic syndrome[J].Can J Physiol Pharmacol,2011,89(12):899-910.
[28]周庆功,刘伟.力竭运动小鼠体内部分元素水平的变化[J].环境与健康杂志,2007,24(12):997-998.
[29]赵敬国,王福文.力竭性运动对心脏影响的研究[J].山东体育科技,2000,22(2):42-46.
[30]郝选明,万文君,黄海,等.补体系统对有氧运动的免疫应答与适应特征[J].中国运动医学杂志,1999,18(3):242-244.
[31]张玮,洪熊.有氧运动对补体的影响[J].中国组织工程研究与临床康复,2007,11(30):6047-6049.
[2]Senthilkumar R,Viswanathan P,Nalini N.Effect of glycine on oxidative stress in rats with alcohol induced liver injury[J].Pharmazie,2004,59(1):55-60.
[3]Barakat HA,Hamza AH.Glycine alleviates liver injury induced by deficiency in methionine and or choline in rats[J].Eur Rev Med Pharmacol Sci,2012,16(6):728-736.
[4]Senthilkumar R,Nalini N.Glycine prevents hepatic fibrosis by preventing the accumulation of collagen in rats with alcoholic liver injury[J].Pol J Pharmacol,2004,56(1):121-128.
[5]Senthilkumar R,Sengottuvelan M,Nalini N.Protective effect of glycine supplementation on the levels of lipid peroxidation and antioxidant enzymes in the erythrocyte of rats with alcohol-induced liver injury[J].Cell Biochem Funct,2004,22(2):123-128.
[6]Senthilkumar R,Nalini N.The potential beneficial effect of glycine on the carbohydrate moieties of glycoproteins in an experimental model of alcohol-induced hepatotoxicity[J].J Med Food,2004,7(1):108-113.
[7]钟孝正,陈琪.甘氨酸对心肌缺血再灌注损伤的保护作用及机制研究[J].中国病理生理杂志,2010,26(10):1953.
[8]秦霞,蒋莉,张咏梅,等.甘氨酸改善缺氧性MDCK细胞损伤的作用依赖于ERK1/2、Akt及p38MARK信号通路[J].南京医科大学学报,2012,32(10):1337-1342.
[9]谢剑梅,杨阁,李建,等.甘氨酸纳米脂质体的制备及其在脑缺血/再灌注中的神经保护作用研究[J].中国药理学通报,2012,28(9):1331-1332.
[10]王国华,姜正林,李霞,等.甘氨酸受体激动剂对大鼠局灶性脑缺血再灌注损伤的神经保护作用[J].临床神经病学杂志,2010,23(1):38-41.
[11]de Aguiar Picanco E,Lopes-Paulo F,Marques RG,et al.L-arginine and glycine supplementation in the repair of the irradiated colonic wall of rats[J].Int J Colorectal Dis,2011,26(5):561-568.
[12]Diestel CF,Marques RG,Lopes-Paulo F,et al.Role of Lglutamine and glycine supplementation on irradiated colonic wall[J].Int J Colorectal Dis,2007,22(12):1523-1529.
[13]武雯,刘近春,李俊华,等.甘氨酸对非酒精性脂肪性肝炎肠组织MIF表达的影响[J].中国病理生理杂志,2010,26(10):2029.
[14]吴惠文,韩德五,许瑞龄,等.甘氨酸对高果糖饮食诱导的大鼠胰岛素抵抗及认知功能损害的影响[J].中国病理生理杂志,2012,28(2):302-307.
[15]李腾,彭靖,吕尚军,等.甘氨酸对烧伤大鼠心肌细胞能量代谢的影响[J].重庆医学,2012,41(33):3476-3478.
[16]彭靖,李腾,吕尚军,等.甘氨酸减轻烧伤大鼠心肌损害的实验研究[J].重庆医学,2012,41(36):3804-3806.
[17]刘宏雁,吕国薇,王维忠.小鼠脑内甘氨酸水平在缺氧预适应中的变化[J].生理学报,2001,53(6):461-464.
[18]张东民,张佳民,马万云,等.急性力竭运动对大鼠下丘脑氨基酸神经递质的影响[J].高等学校化学学报,2002,23(2):230-233.
[19]甘露,黄庆军,张亚超,等.力竭性游泳对小鼠自主行为和免疫功能的影响[J].中国运动医学杂志,2004,23(4):434-435.
[20]武建国.实用临床免疫学检验[M].苏州:江苏科技出版社,1989.
[21]Zhang Y,Lv SJ,Yan H,et al.Effects of glycine supplementation on myocardial damage and cardiac function after severe burn[J].Burns,2013,39(4):729-735.
[22]周军利,黄跃生,党永明.甘氨酸对缺血缺氧心肌细胞能量代谢的影响[J].现代生物医学进展,2008,8(6):1093-1095.
[23]张水军,史冀华,唐哲.甘氨酸对脑死亡大鼠供肝损伤的防护作用[J].中华器官移植杂志,2004,25(5):264-266.
[24]秦杰,谢立平.氨基酸在肾功能保护中的作用研究进展[J].国外医学泌尿系统分册,2003,23(4):374-377.
[25]秦霞,曹剑秋,柏惠,等.甘氨酸对ATP耗竭诱生性细胞自噬的作用及其机制[J].南京医科大学学报,2009,29(12):1633-1637.
[26]Pan C,Bai X,Fan L,et al.Cytoprotection by glycine against ATP-depletion-induced injury is mediated by glycine receptor in renal cells[J].Biochem J,2005,390(Pt2):447-453.
[27]Perez-Torres I,Ibarra B,Soria-Castro E,et al.Effect of glycine on the cyclooxygenase pathway of the kidney arachidonic acid metabolism in a rat model of metabolic syndrome[J].Can J Physiol Pharmacol,2011,89(12):899-910.
[28]周庆功,刘伟.力竭运动小鼠体内部分元素水平的变化[J].环境与健康杂志,2007,24(12):997-998.
[29]赵敬国,王福文.力竭性运动对心脏影响的研究[J].山东体育科技,2000,22(2):42-46.
[30]郝选明,万文君,黄海,等.补体系统对有氧运动的免疫应答与适应特征[J].中国运动医学杂志,1999,18(3):242-244.
[31]张玮,洪熊.有氧运动对补体的影响[J].中国组织工程研究与临床康复,2007,11(30):6047-6049.