摘要
目的探讨甘氨酸补充对一次力竭性运动小鼠部分生化指标的影响。方法将雄性昆明小鼠分为3组,每组10只:力竭性游泳运动组(E组),甘氨酸补充后力竭性游泳组(G组)及静息正常对照组(S组)。G组小鼠采用甘氨酸灌胃处理4周,其余两组给予生理盐水。4周后,E组和G组均做力竭性游泳运动。采样后进行补体溶血活性测定,并测定血清中部分生化指标的水平。结果与E组比较,G组的力竭性游泳运动时间显著延长(P<0.01)。力竭性游泳后,E组小鼠血清中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)、乳酸脱氢酶(LDH)、肌酸激酶(CK)、尿素氮、血清铁(Fe3+)和补体溶血活性(CHA)的水平较S组发生了显著性的变化(P<0.01),与E组比较,G组小鼠血清中上述生化指标得到了显著改善(P<0.01),其中ALP和LDH的水平可以恢复到与S组相当的水平(P>0.05)。结论力竭性游泳小鼠血清部分生化指标异常,甘氨酸预补充对这些异常有明显的改善作用,可以一定程度地提高小鼠的运动能力。
Objective To investigate the influence of glycine supplement on partial biochemical indicator in exhaustive exercise mice.Methods Divided Kunming′s male-mice into 3group(10/group):exhaustive swimming group(E),exhaustive swimming group with glycine supplement(G)and silent control group(S).Mice in the G group was treated with 4-weeked intragastric administration of glycine,while mice in other 2groups received physiological saline.After 4weeks,mice in E and G group did the exhaustive swimming exercise.After sampling,the activity of complement hemolytic activity was measured,and the serum levels of some biochemical indexes were determined.Results Time of exhaustive swimming in G group was significantly longer than E group(P<0.01).After exhaustive swimming,the ALT,AST,ALP,LDH,CK,N,Fe3+and CHA in E group were significantly changed compared with S group(P<0.01);while those indicators in G group were significantly improved than E group(P<0.01),the ALP and LDH level can regain to the level of S group(P>0.05).Conclusion Exhaustive swimming mice can have part indicator abnormality,but Glycine supplement can effectively improve that condition and enhance the exercise ability to some extent.
引文
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