基于“肾藏精起亟”理论观察补肾活血中药对慢性心力衰竭大鼠骨桥蛋白影响
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摘要
目的:基于"肾藏精起亟"理论观察补肾活血中药对慢性心力衰竭(CHF)大鼠骨桥蛋白(OPN)的影响。方法:60只雄性SD大鼠,随机选取15只为假手术组,剩余45只通过冠脉结扎配合力竭式游泳建立CHF大鼠模型,模型成功后随机分为模型组、西药组、中药组。用药4周后,超声检测大鼠心脏形态变化,免疫组化法测定大鼠心肌及肾脏组织中OPN表达水平,Real-time PCR法检测大鼠心肌及肾脏组织中OPN mRNA表达水平。结果:治疗后,与假手术组比较,模型组大鼠左室内径(LVID)显著增高,射血分数(EF)和短轴缩短率(FS)均显著减低(P<0.05),心、肾组织中OPN蛋白及mRNA表达水平显著升高(P<0.05);与模型组比较,西药组、中药组LVID显著降低,EF、FS显著升高(P<0.05),心、肾组织中OPN蛋白及mRNA表达水平显著降低(P<0.05)。结论:补肾活血中药可下调OPN蛋白及mRNA的表达,改善CHF大鼠心室重构,揭示了"肾藏精起亟"心肾相关的中医病机基础。
Objective: To observe the effect of Bushen Huoxue Formula on the OPN in chronic heart failure rats based on the theory of ‘the kidney's function of storage the essence of life for accommodation'. Methods: Sixty male SD rats were randomly selected as sham operation group, and the remaining 45 rats were randomly divided into model group, western medicine group and traditional Chinese medicine group after successful model establishment by coronary artery ligation and exhaustive swimming. After 4 weeks of treatment, the heart morphology of rats was detected with ultrasound examination, OPN expression in myocardium and kidney of rats was detected with immunohistochemistry, OPN mRNA expression in myocardium and kidney of rats was detected with Real-time PCR. Results: After treatment, compared with sham operation group, left ventricular internal diameter(LVID) of model group rats were increased significantly, ejection fraction(EF) and fractional shortening(FS) of model group rats were decreased significantly(P<0.05), OPN and OPN mRNA expression in myocardium and kidney of model group rats were increased significantly(P<0.05). Compared with model group, LVID of lisinopril and Bushen Huoxue group rats were decreased significantly, EF and FS of lisinopril and Bushen Huoxue group rats were increased significantly(P<0.05), OPN and OPN mRNA expression in myocardium and kidney of lisinopril and Bushen Huoxue group rats were decreased significantly(P<0.05). Conclusion: Bushen Huoxue Formula can reduce the expression of OPN mRNA and OPN, and improve the ventricular remodeling of chronic heart failure rats, reveal traditional Chinese medicine pathogenesis of ‘kidney's function of storage the essence of life for accommodation' with heart and kidney related theory.
Objective: To observe the effect of Bushen Huoxue Formula on the OPN in chronic heart failure rats based on the theory of ‘the kidney's function of storage the essence of life for accommodation'. Methods: Sixty male SD rats were randomly selected as sham operation group, and the remaining 45 rats were randomly divided into model group, western medicine group and traditional Chinese medicine group after successful model establishment by coronary artery ligation and exhaustive swimming. After 4 weeks of treatment, the heart morphology of rats was detected with ultrasound examination, OPN expression in myocardium and kidney of rats was detected with immunohistochemistry, OPN mRNA expression in myocardium and kidney of rats was detected with Real-time PCR. Results: After treatment, compared with sham operation group, left ventricular internal diameter(LVID) of model group rats were increased significantly, ejection fraction(EF) and fractional shortening(FS) of model group rats were decreased significantly(P<0.05), OPN and OPN mRNA expression in myocardium and kidney of model group rats were increased significantly(P<0.05). Compared with model group, LVID of lisinopril and Bushen Huoxue group rats were decreased significantly, EF and FS of lisinopril and Bushen Huoxue group rats were increased significantly(P<0.05), OPN and OPN mRNA expression in myocardium and kidney of lisinopril and Bushen Huoxue group rats were decreased significantly(P<0.05). Conclusion: Bushen Huoxue Formula can reduce the expression of OPN mRNA and OPN, and improve the ventricular remodeling of chronic heart failure rats, reveal traditional Chinese medicine pathogenesis of ‘kidney's function of storage the essence of life for accommodation' with heart and kidney related theory.
引文
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[12]白斌.从肾论治慢性心力衰竭.实用中医内科杂志,2016,30(4):44-45
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[15]杜磊,郑洪新.肾藏精起亟舍志与在志为恐.辽宁中医药大学学报,2012,14(7):67-68
[16]王辰,张艳,宫丽鸿.参草通脉颗粒治疗气虚血瘀水停型慢性心力衰竭的临床研究.中华中医药杂志,2012,27(10):2521-2523
[2]Uede T,Katagiri Y,lizuka J,et al.Osteopntin,a coordinator of host defense sestem:a cytokine or an extracellular adhesive protein?.Microbiol Innunol,1997,41:641-648
[3]陈雷,张旗.心力衰竭患者血清OPN与BNP、CRP水平及心功能的关系.中国医药指南,2017,15(5):109-109
[4]曹刚,全红,钟德福.螺内酯治疗急性心肌梗死后心力衰竭的效果及骨桥蛋白的变化.中国医药导报,2016,13(6):161-162
[5]礼海.益气活血复方对慢性心衰大鼠心肌MMP-1、TIMP-1影响相关性及抗心室重构机制的研究.沈阳:辽宁中医药大学,2010
[6]马金.益气活血复方联合运动训练对慢性心衰大鼠的心功能及左室重构影响的实验研究.沈阳:辽宁中医药大学,2011
[7]廖佳丹,张艳,宫丽鸿,等.益气活血利水不同配伍对慢性心衰大鼠AT1、ERK2影响的实验研究.中华中医药学刊,2012,30(10):2270-2273
[8]韩安邦,路迎冬,张丹丹,等.益气活血方对慢性心力衰竭大鼠肺组织水通道蛋白表达的影响.中华中医药杂志,2017,32(10):4395-4396
[9]邢作英,慢性心力衰竭流行病学研究现状及其病因.中华实用诊断与治疗杂志,2012,26(10):937-937
[10]Lindsey M L,Mann D L,Entman M L,et al.Extracellular matrix remodeling following myocardial injury.Ann Med,2001,88:1080-1082
[11]Yutaka Matsui,Nan Jia,Hiroshi Okamoto,et al.Roleof osteopontin in cardiac fibrosis and remodeling in angiotensinI I-induced cardiac hypertrophy.Hypertension,2004,43:1195
[12]白斌.从肾论治慢性心力衰竭.实用中医内科杂志,2016,30(4):44-45
[13]赵殿臣,张艳.运用经方从心肾论治慢性心力衰竭.辽宁中医药大学学报,2011,13(7):174-175
[14]张鹏,施杞,王拥军.“阴者,藏精而起亟也”新解.中医杂志,2013,54(17):1519-1520
[15]杜磊,郑洪新.肾藏精起亟舍志与在志为恐.辽宁中医药大学学报,2012,14(7):67-68
[16]王辰,张艳,宫丽鸿.参草通脉颗粒治疗气虚血瘀水停型慢性心力衰竭的临床研究.中华中医药杂志,2012,27(10):2521-2523