稻瘟病菌NUDIX水解酶家族的生物信息学及表达特性研究
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摘要
NUDIX水解酶家族能够清除和调节细胞内产生的有害代谢产物,在机体保护、调节和信号传递等过程中发挥重要的生物学功能,大豆疫霉中的无毒蛋白Avr3b就具有NUDIX水解酶活性。为了探究稻瘟病菌中NUDIX水解酶家族的生物学功能,我们借助生物信息学手段对稻瘟病菌中鉴定出的18个NUDIX蛋白的结构、理化性质、系统进化关系进行了分析,结合RNA-seq和RT-PCR数据,我们发现其中的两个蛋白NUDIX15-1和NUDIX15-2同大豆疫霉无毒蛋白Avr3b高度同源,极有可能在稻瘟病菌的侵染过程中发挥功能。
NUDIX hydrolase family can eliminate and regulate the harmful metabolites produced by organisms and plays an important role in cell protection, regulation and signal transduction. Avirulence protein Avr3 b from oomycete Phytophthora sojae acts as a NUDIX hydrolase as well. To determine the biology function of NUDIX hydrolase family in Magnaporthe oryzae, we identified 18 NUDIX proteins in M. oryzae and analyzed their structures, physical and chemical properties and phylogenetic relationship by bioinformatics methods. Combined with the data from RNA-seq and RT-PCR, we found two of the members NUDIX15-1 and NUDIX15-2 revealed high similarities with Avr3 b in P. sojae, which indicated these two members could play a crucial function in the early infectious stages of M. oryzae.
NUDIX hydrolase family can eliminate and regulate the harmful metabolites produced by organisms and plays an important role in cell protection, regulation and signal transduction. Avirulence protein Avr3 b from oomycete Phytophthora sojae acts as a NUDIX hydrolase as well. To determine the biology function of NUDIX hydrolase family in Magnaporthe oryzae, we identified 18 NUDIX proteins in M. oryzae and analyzed their structures, physical and chemical properties and phylogenetic relationship by bioinformatics methods. Combined with the data from RNA-seq and RT-PCR, we found two of the members NUDIX15-1 and NUDIX15-2 revealed high similarities with Avr3 b in P. sojae, which indicated these two members could play a crucial function in the early infectious stages of M. oryzae.
引文
Bartsch M.,Gobbato E.,Bednarek P.,Debey S.,Schultze J.L.Bautor J.,and Parker J.E.,2006,Salicylic acid-independen enhanced disease susceptibility1 signaling in Arabidopsi immunity and cell death is regulated by the monooxygenas FMO1 and the Nudix hydrolase NUDT7,Plant Cell,18(4)1038-1051
Bessman M.J.,Frick D.N.,and O'Handley S.F.,1996,The MutTproteins or“Nudix”hydrolases,a family of versatile,widely distributed“,housecleaning”enzymes,The Journal of Bio logical Chemistry,271(41):25059-25062
Dean R.A.,Talbot N.J.,Ebbole D.J.,Farman M.L.,Mitchell T.KOrbach M.J.,Thon M.,Kulkarni R.,Xu J.R.,Pan H.Q.Read N.D.,Lee Y.H.,Carbone I.,Brown D.,Oh Y.Y.Donofrio N.,Jeong J.S.,Soanes D.M.,Djonovic S.,Kolomi ets E.,Rehmeyer C.,Li W.X.,Harding M.,Kim S.,Lebrun M.H.,Bohnert H.,Coughlan S.,Butler J.,Calvo S.,Ma L.J.Nicol R.,Purcell S.,Nusbaum C.,Galagan J.E.,and Birren B.W.,2005,The genome sequence of the rice blast fungu Magnaporthe grisea,Nature,434(7036):980-986
Dong S.M.,Yin W.X.,Kong G.H.,Yang X.Y.,Qutob D.A.,Chen Q.H.,Kale S.D.,Sui Y.Y.,Zhang Z.G.,Dou D.L.,Zheng XB.,Gijzen M.,Tyler B.M.,and Wang Y.C.,2011,Phytophthora sojae avirulence effector Avr3b is a secreted NADHand ADP-ribose pyrophosphorylase that modulates plan immunity,Plo S Pathogens,7(11):e1002353
Giraldo M.C.,Dagdas Y.F.,Gupta Y.K.,Mentlak T.A.,Yi M.Martinez-Rocha A.L.,Saitoh H.,Terauchi R.,Talbot N.J.and Valent B.,2013,Two distinct secretion systems facili tate tissue invasion by the rice blast fungus Magnaporthe oryzae,Nature Communications,13(3):156-160
Kong G.H.,Zhao Y.,Jing M.F.,Huang J.,Yang J.,Xia Y.Q.Kong L.,Ye W.W.,Xiong Q.,Qiao Y.L.,Dong S.M.,MW.B.,and Wang Y.C.,2015,The activation of Phytophthor effector Avr3b by plant cyclophilin is required for the Nudix hydrolase activity of Avr3b,PLo S Pathogens,11(8):e1005-139
Kraszewska E.,2008,The plant Nudix hydrolase family,Act Biochimica Polonica,55(4):663-671
Mclennan A.G.,2006,The nudix hydrolase superfamily,Cellula and Molecular Life Sciences,63(2006):123-143
Mildvan A.S.,Xia Z.,Azurmendi H.F.,Saraswat V.,Legler P.M.Massiah M.A.,Gabelli S.B.,Bianchet M.A.,Kang L.W.,and Amzel L.M.,2005,Structures and mechanisms of Nudix hy drolases,Archives of Biochemistry and Biophysics,433(1)129-143
Ogawa T.,Ueda Y.,Yoshimura K.,and Shigeoka S.,2005,Com prehensive analysis of cytosolic Nudix hydrolases in Arabid opsis thaliana,The Journal of Biological Chemistry,280(26):25277-25283
Tamura N.,Murata Y.,and Mukaihara T.,2005,Isolation of Ralstonia solanacearum hrp B constitutive mutants and secretion analysis of hrp B-regulated gene products that share homolo gy with known type III effectors and enzymes,Microbiolo gy,151(Pt 9):2873-2884
Xu W.L.,Dunn C.A.,Jones C.R.,D'Souza G.,and Bessman M.J.2004,The 26 Nudix hydrolases of Bacillus cereus,a clos relative of Bacillus anthracis,The Journal of Biological Che mistry,279(23):24861-24865
Yoshimura K.,and Shigeoka S.,2015,Versatile physiologica functions of the Nudix hydrolase family in Arabidopsis,Bio science,Biotechnology,and Biochemistry,79(3):354-366
Zhang X.C.,Wu K.X.,Li W.B.,Xia Y.J.,and Peng M.,2012Cloning and function analysis of promoter AtN UDT8 in Arabidopsis thaliana,Redai Zuowu Xuebao(Chinese Journal of Tropical Crops),33(2):116-120(张秀春,吴坤鑫,李文彬,夏亦荠,彭明,2012,拟南芥AtN UDT8启动子的分离及其功能分析,热带作物学报,33(2):116-120)
Bessman M.J.,Frick D.N.,and O'Handley S.F.,1996,The MutTproteins or“Nudix”hydrolases,a family of versatile,widely distributed“,housecleaning”enzymes,The Journal of Bio logical Chemistry,271(41):25059-25062
Dean R.A.,Talbot N.J.,Ebbole D.J.,Farman M.L.,Mitchell T.KOrbach M.J.,Thon M.,Kulkarni R.,Xu J.R.,Pan H.Q.Read N.D.,Lee Y.H.,Carbone I.,Brown D.,Oh Y.Y.Donofrio N.,Jeong J.S.,Soanes D.M.,Djonovic S.,Kolomi ets E.,Rehmeyer C.,Li W.X.,Harding M.,Kim S.,Lebrun M.H.,Bohnert H.,Coughlan S.,Butler J.,Calvo S.,Ma L.J.Nicol R.,Purcell S.,Nusbaum C.,Galagan J.E.,and Birren B.W.,2005,The genome sequence of the rice blast fungu Magnaporthe grisea,Nature,434(7036):980-986
Dong S.M.,Yin W.X.,Kong G.H.,Yang X.Y.,Qutob D.A.,Chen Q.H.,Kale S.D.,Sui Y.Y.,Zhang Z.G.,Dou D.L.,Zheng XB.,Gijzen M.,Tyler B.M.,and Wang Y.C.,2011,Phytophthora sojae avirulence effector Avr3b is a secreted NADHand ADP-ribose pyrophosphorylase that modulates plan immunity,Plo S Pathogens,7(11):e1002353
Giraldo M.C.,Dagdas Y.F.,Gupta Y.K.,Mentlak T.A.,Yi M.Martinez-Rocha A.L.,Saitoh H.,Terauchi R.,Talbot N.J.and Valent B.,2013,Two distinct secretion systems facili tate tissue invasion by the rice blast fungus Magnaporthe oryzae,Nature Communications,13(3):156-160
Kong G.H.,Zhao Y.,Jing M.F.,Huang J.,Yang J.,Xia Y.Q.Kong L.,Ye W.W.,Xiong Q.,Qiao Y.L.,Dong S.M.,MW.B.,and Wang Y.C.,2015,The activation of Phytophthor effector Avr3b by plant cyclophilin is required for the Nudix hydrolase activity of Avr3b,PLo S Pathogens,11(8):e1005-139
Kraszewska E.,2008,The plant Nudix hydrolase family,Act Biochimica Polonica,55(4):663-671
Mclennan A.G.,2006,The nudix hydrolase superfamily,Cellula and Molecular Life Sciences,63(2006):123-143
Mildvan A.S.,Xia Z.,Azurmendi H.F.,Saraswat V.,Legler P.M.Massiah M.A.,Gabelli S.B.,Bianchet M.A.,Kang L.W.,and Amzel L.M.,2005,Structures and mechanisms of Nudix hy drolases,Archives of Biochemistry and Biophysics,433(1)129-143
Ogawa T.,Ueda Y.,Yoshimura K.,and Shigeoka S.,2005,Com prehensive analysis of cytosolic Nudix hydrolases in Arabid opsis thaliana,The Journal of Biological Chemistry,280(26):25277-25283
Tamura N.,Murata Y.,and Mukaihara T.,2005,Isolation of Ralstonia solanacearum hrp B constitutive mutants and secretion analysis of hrp B-regulated gene products that share homolo gy with known type III effectors and enzymes,Microbiolo gy,151(Pt 9):2873-2884
Xu W.L.,Dunn C.A.,Jones C.R.,D'Souza G.,and Bessman M.J.2004,The 26 Nudix hydrolases of Bacillus cereus,a clos relative of Bacillus anthracis,The Journal of Biological Che mistry,279(23):24861-24865
Yoshimura K.,and Shigeoka S.,2015,Versatile physiologica functions of the Nudix hydrolase family in Arabidopsis,Bio science,Biotechnology,and Biochemistry,79(3):354-366
Zhang X.C.,Wu K.X.,Li W.B.,Xia Y.J.,and Peng M.,2012Cloning and function analysis of promoter AtN UDT8 in Arabidopsis thaliana,Redai Zuowu Xuebao(Chinese Journal of Tropical Crops),33(2):116-120(张秀春,吴坤鑫,李文彬,夏亦荠,彭明,2012,拟南芥AtN UDT8启动子的分离及其功能分析,热带作物学报,33(2):116-120)