稻瘟病菌中蛋白激酶CK2的功能预测分析
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摘要
通过生物信息学分析,初步掌握了稻瘟病菌(Magnaporthe oryzae)中蛋白激酶CK2三亚基的结构特点,生物进化树分析表明CK2的蛋白序列在进化上的高度保守性;利用酵母同源比对的方法预测分析了稻瘟病菌中潜在的互作底物,揭示了CK2可能参与的生理生化过程,并且重点预测分析了CK2与小G蛋白Rho3之间的互作关系,为进一步揭示稻瘟病菌蛋白激酶CK2的功能及其参与调控的信号通路提供了理论基础。
Protein kinase CK2,a conservative serine/threonine kinase,is typically found in tetrameric complexes consisting of two catalytic subunits and two regulatory subunits.The structural features of CK2 in Magnaporthe oryzae were analyzed with bio-informatics.The cladograms analysis revealed that the protein sequences of CK2 is very conservative in the evolution process.Yeast homologous comparison implied that CK2 potential substrates were forecasted in M.oryzae,meaning CK2 participated in some the physiological and biochemical processes.The interaction between CK2 and Rho3 was emphasized.The study is a good way to further study the function and signal transduction pathway of protein kinase CK2 in M.oryzae.
Protein kinase CK2,a conservative serine/threonine kinase,is typically found in tetrameric complexes consisting of two catalytic subunits and two regulatory subunits.The structural features of CK2 in Magnaporthe oryzae were analyzed with bio-informatics.The cladograms analysis revealed that the protein sequences of CK2 is very conservative in the evolution process.Yeast homologous comparison implied that CK2 potential substrates were forecasted in M.oryzae,meaning CK2 participated in some the physiological and biochemical processes.The interaction between CK2 and Rho3 was emphasized.The study is a good way to further study the function and signal transduction pathway of protein kinase CK2 in M.oryzae.
引文
[1]Baker B,Zambryski P,Taskawicz B,et a1.Signaling in plant-Microbe interactions[J].Science,1997,276(5 313):726-733.
[2]Talbot N J.On the trail of a cereal killer:ExPloring the biologyof Magnaporthe grisea[J].Annual Review of Microbiology,2003,57:177-202.
[3]Burnett G,Kennedy,E P The enzymatic phosphorylation ofproteins[J].Journal of Biological Chemistry,1954,211(2):969-980.
[4]Niefind K,Raaf J,Issinger OG.Protein kinase CK2 in healthand disease:Protein kinase CK2:from structures to insights[J].Cell Mol Life Sci,2009,66(11-12):1800-16.doi:10.1007/s00018-009-9149-8
[5]Litchfield D W.Protein kinase CK2:structure,regulation androle in cellular decisions of life and death[J].Biochem J,2003,369(Pt 1):1-15.
[6]Allende J E,Allende CC.Protein kinases.4.Protein kinase CK2:an enzyme with multiple substrates and a puzzling regulation[J].FASEB J,1995,9(5):313-323
[7]Glover C V 3rd.On the physiological role of casein kinase IIin Saccharomyces cerevisiae[J].Prog Nucleic Acid Res MolBiol,1998,59:95-133.
[8]Padmanabha R,Chen-Wu JL,Hanna DE,et al.Isolation,sequencing,and disruption of the yeast CKA2 gene:caseinkinase II is essential for viability in Saccharomycescerevisiae[J].Mol Cell Biol,1990,10(8):4 089-4 099.
[9]Mulekar J J,Huq E.Does CK2 affect flowering time bymodulating the autonomous pathway in Arabidopsis?[J].PlantSignal Behav,2012,7(2):292-294.
[10]Pinna L A,Allende J E.Protein kinase CK2 in health anddisease:Protein kinase CK2:an ugly duckling in the kinomepond[J].Cell Mol Life Sci,2009,66(11-12):1 795-1 799.
[11]陈小文,刘新光,梁念慈,等.蛋白激酶CK2[J].国外医学分子生物学分册,2001,23(4):206-209.
[12]林雄杰,周洁,王宗华,等.稻瘟病菌几丁质结合蛋白的生物信息学分析[J].中国农学通报,2011,27(21):239-245.
[13]Meggio F,Pinna L A.One thousand and one substrates ofprotein kinase CK2[J].FASEB J,2003,17(3):349-368..
[14]Dotan I,Ziv E,Dafni N,et al.Functional conservation betweenthe human,nematode,and yeast CK2 cell cycle genes[J].Biochem Biophys Res Commun,2001,288(3):603-609.
[15]Riera M,Irar S,Vélez-Bermúdez IC,et al.Role of plant-specific N-terminal domain of maize CK2b1 subunit in CK2bfunctions and holoenzyme regulation[J].PLoS ONE,20116(7):e21909.
[16]Drees BL,Sundin B,Brazeau E,et al.A protein interactionmap for cell polarity development[J].J Cell Biol,2001,154(3):549-571.
[2]Talbot N J.On the trail of a cereal killer:ExPloring the biologyof Magnaporthe grisea[J].Annual Review of Microbiology,2003,57:177-202.
[3]Burnett G,Kennedy,E P The enzymatic phosphorylation ofproteins[J].Journal of Biological Chemistry,1954,211(2):969-980.
[4]Niefind K,Raaf J,Issinger OG.Protein kinase CK2 in healthand disease:Protein kinase CK2:from structures to insights[J].Cell Mol Life Sci,2009,66(11-12):1800-16.doi:10.1007/s00018-009-9149-8
[5]Litchfield D W.Protein kinase CK2:structure,regulation androle in cellular decisions of life and death[J].Biochem J,2003,369(Pt 1):1-15.
[6]Allende J E,Allende CC.Protein kinases.4.Protein kinase CK2:an enzyme with multiple substrates and a puzzling regulation[J].FASEB J,1995,9(5):313-323
[7]Glover C V 3rd.On the physiological role of casein kinase IIin Saccharomyces cerevisiae[J].Prog Nucleic Acid Res MolBiol,1998,59:95-133.
[8]Padmanabha R,Chen-Wu JL,Hanna DE,et al.Isolation,sequencing,and disruption of the yeast CKA2 gene:caseinkinase II is essential for viability in Saccharomycescerevisiae[J].Mol Cell Biol,1990,10(8):4 089-4 099.
[9]Mulekar J J,Huq E.Does CK2 affect flowering time bymodulating the autonomous pathway in Arabidopsis?[J].PlantSignal Behav,2012,7(2):292-294.
[10]Pinna L A,Allende J E.Protein kinase CK2 in health anddisease:Protein kinase CK2:an ugly duckling in the kinomepond[J].Cell Mol Life Sci,2009,66(11-12):1 795-1 799.
[11]陈小文,刘新光,梁念慈,等.蛋白激酶CK2[J].国外医学分子生物学分册,2001,23(4):206-209.
[12]林雄杰,周洁,王宗华,等.稻瘟病菌几丁质结合蛋白的生物信息学分析[J].中国农学通报,2011,27(21):239-245.
[13]Meggio F,Pinna L A.One thousand and one substrates ofprotein kinase CK2[J].FASEB J,2003,17(3):349-368..
[14]Dotan I,Ziv E,Dafni N,et al.Functional conservation betweenthe human,nematode,and yeast CK2 cell cycle genes[J].Biochem Biophys Res Commun,2001,288(3):603-609.
[15]Riera M,Irar S,Vélez-Bermúdez IC,et al.Role of plant-specific N-terminal domain of maize CK2b1 subunit in CK2bfunctions and holoenzyme regulation[J].PLoS ONE,20116(7):e21909.
[16]Drees BL,Sundin B,Brazeau E,et al.A protein interactionmap for cell polarity development[J].J Cell Biol,2001,154(3):549-571.