闽北十县市钩藤中钩藤碱与异钩藤碱的含量比较研究
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摘要
目的通过比较分析福建闽北地区十县市的钩藤资源中钩藤碱与异钩藤碱的含量情况,为筛选闽北地区良好的钩藤种质资源提供依据。方法采用高效液相色谱方法测定不同种质资源钩藤中钩藤碱与异钩藤碱的含量。结果不同种质资源的钩藤中钩藤碱与异钩藤碱的含量差异性较大,其中钩藤碱与异钩藤碱含量最高的是武夷山的钩藤,含量最低的是松溪县的钩藤。结论不同种质来源的钩藤中钩藤碱与异钩藤碱的含量差异较大,提示引种闽北各县市钩藤时应注意不同种质资源来源,同时为福建闽北本土的钩藤引种提供依据。
Objective: To investigate the rhynchophylline and isorhynchophylline contents in Uncaria rhynchophylla of various germplasm resources. Methods: Rhynchophylline and isorhynchophylline contents in U.rhynchophylla of various germplasm resources were determined using high-performance liquid chromatography(HPLC). Results: There were great differences about rhynchophylline and isorhynchophylline contents among various germplasm resources, the highest contents were determined in the sample from Wuyishan, the lowest from Songxi. Conclusion: The significant differences among various germplasm resources of U. rhynchophylla suggested that attention should be paid to different sources of germplasm resources when the introduction was carried out, and the results could provide theoretical basis for the introduction of local U. rhynchophylla in northern Fujian.
Objective: To investigate the rhynchophylline and isorhynchophylline contents in Uncaria rhynchophylla of various germplasm resources. Methods: Rhynchophylline and isorhynchophylline contents in U.rhynchophylla of various germplasm resources were determined using high-performance liquid chromatography(HPLC). Results: There were great differences about rhynchophylline and isorhynchophylline contents among various germplasm resources, the highest contents were determined in the sample from Wuyishan, the lowest from Songxi. Conclusion: The significant differences among various germplasm resources of U. rhynchophylla suggested that attention should be paid to different sources of germplasm resources when the introduction was carried out, and the results could provide theoretical basis for the introduction of local U. rhynchophylla in northern Fujian.
引文
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[6]王晓颖,刘永静,陈丹,等.HPLC法测定不同产地钩藤中钩藤碱的含量分析[J].中国中医药科技,2011,18(3):215-216.
[2]王瑞俭,孙广仁,孙宝民.钩藤碱提取工艺研究[J].吉林林学院学报,1998,14(2):109-111.
[3]原田正敏.日本常用中药的定量分析-防己、钩藤[J].国外医药-植物药分册,1991,6(5):219.
[4]王英峰,魏璐雪,王保中.高效液相色谱法测定钩藤中钩藤碱、异钩藤碱含量[J].药物分析杂志,1999,19(1):56-57.
[5]李思蒙,侴桂新.钩藤药材中异钩藤碱含量的超高效液相色谱和高效液相色谱法测定[J].时珍国医国药,2010,21(12):3195-3197.
[6]王晓颖,刘永静,陈丹,等.HPLC法测定不同产地钩藤中钩藤碱的含量分析[J].中国中医药科技,2011,18(3):215-216.