肉苁蓉中多酚类物质提取条件的优化及含量的测定
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摘要
本研究建立了紫外-可见分光光度法测定肉苁蓉中多酚类物质(以没食子酸计)含量的方法。当乙醇浓度为70%、温度为60℃、提取时间为30min、料液比为1:20时,肉苁蓉中多酚类物质(以没食子酸计)的提取效果最好。同时肉苁蓉样品中多酚类物质(以没食子酸计)的平均质量百分比为0.2422%。精密度、稳定性和加标回收结果显示该方法可行可靠。
A method for the determination of polyphenols in Cistanche was established by ultraviolet-visible spectrophotom?etry. The results indicate that the extraction rate is best when the concentration of ethanol is 70%;the temperature is 60 °C;the extraction time is 30 min;the ratio of material to liquid is 1:20. Meanwhile,the average mass percentage of polyphe?nols in Cistanche deserticola samples is 0.2422%. The results of precision,stability and recovery show that the method is feasible and reliable.
A method for the determination of polyphenols in Cistanche was established by ultraviolet-visible spectrophotom?etry. The results indicate that the extraction rate is best when the concentration of ethanol is 70%;the temperature is 60 °C;the extraction time is 30 min;the ratio of material to liquid is 1:20. Meanwhile,the average mass percentage of polyphe?nols in Cistanche deserticola samples is 0.2422%. The results of precision,stability and recovery show that the method is feasible and reliable.
引文
[1]汪燕平,余新忠,宋元.以降本草书写与地道药材建构讨论[J],安徽大学学报,哲学社会科学版2018,3:92-101.
[2]李保定,肉苁蓉炖鸡风味形成研究[J],中国调味品,2017,42(4):96-102.
[3]石碧,狄莹.植物多酚[M].北京:科学出版社,2000:19.
[4]雷昌贵,陈锦屏,卢大新等.食品中多酚化合物的测定方法及研究进展[J],食品与发酵业,2007,33(1):100-104.
[5]王德吉,田笠卿,王连生.原子吸收在有机化学中的应用(Ⅲ):茶叶中单宁的测定[J].高等学校化学学报,1995,16(4):536-539.
[6]赵萍,王雅,王晓明等.锁阳多酚类物质的提取及抗氧化性研究[J],学术论坛.CFI(12)DEC 2009.46-48.
[2]李保定,肉苁蓉炖鸡风味形成研究[J],中国调味品,2017,42(4):96-102.
[3]石碧,狄莹.植物多酚[M].北京:科学出版社,2000:19.
[4]雷昌贵,陈锦屏,卢大新等.食品中多酚化合物的测定方法及研究进展[J],食品与发酵业,2007,33(1):100-104.
[5]王德吉,田笠卿,王连生.原子吸收在有机化学中的应用(Ⅲ):茶叶中单宁的测定[J].高等学校化学学报,1995,16(4):536-539.
[6]赵萍,王雅,王晓明等.锁阳多酚类物质的提取及抗氧化性研究[J],学术论坛.CFI(12)DEC 2009.46-48.