强脉冲光对大鼠Ⅱ度烫伤模型的建立
|
摘要
为探索脉冲光对大鼠深Ⅱ度烫伤模型建立的实验条件,将24只大鼠随机分成四组,每组6只,选取大鼠脊柱两侧皮肤作为烫伤部位。第1组为正常对照组,第2、3、4组烫伤条件分别为强脉冲光照射7次、8次和9次,每次能量为20 J/cm~2。建立模型后并进行组织形态学检查。结果表明:第2组大鼠为Ⅰ度烫伤,第3组大鼠为深Ⅱ度烫伤,第4组为Ⅲ度烧伤。大鼠模型制作过程中,烫伤的程度同时受照射次数、能量的影响。实验中所建立的大鼠深Ⅱ度烫伤模型制备方法可以通过控制光照强度和次数,利用强脉冲光建立模型操作简单,制造的损伤程度、损伤面积均匀,重复性好。
To explore the experimental conditions of intense pulsed light on deep second degree scald in rats,twenty-four rats were randomly divided into four groups with 6 rats in each group. The skin on both sides of the spine was selected as the scald site. The first group was normal control group,the second,third and fourth group scald conditions were intense pulsed light irradiation 7 times,8 times and 9 times,each energy 20 J/cm~2,the model was established and histomorphological examination. The results show that the rats in group 2 were scalded for degree Ⅰ,the rats in group 3 were deep Ⅱ degree scalds,and the rats in group 4 were Ⅲ degree burns. In the process of rat model making,the degree of scald is affected by the number of irradiation and the energy at the same time. The deep Ⅱ degree scald rat model established can control the light intensity and the number of times,the use of pulsed light to establish the model is simple,the degree of damage made,the damage area is uniform,good repeatability.
To explore the experimental conditions of intense pulsed light on deep second degree scald in rats,twenty-four rats were randomly divided into four groups with 6 rats in each group. The skin on both sides of the spine was selected as the scald site. The first group was normal control group,the second,third and fourth group scald conditions were intense pulsed light irradiation 7 times,8 times and 9 times,each energy 20 J/cm~2,the model was established and histomorphological examination. The results show that the rats in group 2 were scalded for degree Ⅰ,the rats in group 3 were deep Ⅱ degree scalds,and the rats in group 4 were Ⅲ degree burns. In the process of rat model making,the degree of scald is affected by the number of irradiation and the energy at the same time. The deep Ⅱ degree scald rat model established can control the light intensity and the number of times,the use of pulsed light to establish the model is simple,the degree of damage made,the damage area is uniform,good repeatability.
引文
1 Abdullahi A,Amini-Nik S,Jeschke M G.Animal models in burn research.Cellular and Molecular Life Sciences,2014;71(17):3241-3255
2 Negishi K,Wakamatsu S,Kushikata N,et al.Full-face photorejuvenation of photodamaged skin by intense pulsed light with integrated contact cooling:Initial experiences in Asian patients.Lasers in Surgery and Medicine,2002;30(4):298-305
3陈孝平,汪建平.外科学.8版.北京:人民卫生出版社,2013:360-362Chen Xiaoping,Wang Jianping.Surgery.8th ed.Beijing:People's Health Publishing House,2013:360-362
4张大伟,龚震宇,彭毅志.大鼠烫伤创面感染模型的研制.中华烧伤杂志,2011;(2):104-108Zhang Dawei,Gong Zhenyu,Peng Yizhi.Development of rat scald wound infection model.Chinese Journal of Burns,2011;(2):104-108
5刘颖,陈镇,夏泉,等.烧伤膏对烫伤小鼠创面病理修复作用的影响.中国中医急症,2011;20(5):746-748Liu Ying,Chen Zhen,Xia Quan,et al.Effects of burn ointment on pathological repair of wounds in scalded mice.Chinese Journal of Emergency Medicine,2011;20(5):746-748
6曹蔚,崔芬芳,谢艳华,等.椒目仁油合用盐酸小檗碱治疗烫伤的研究.科学技术与工程,2011;11(36):8970-8973Cao Wei,Cui Fenfang,Xie Yanhua,et al.Study on the treatment of scald with berberine hydrochloride combined with Jiao Mu Ren You.Science Technology and Engineering,2011;11(36):8970-8973
7姚庆君,贾赤宇,陈璧,等.一种大鼠蒸气烫伤模型的建立.中华烧伤杂志,2004;(3):168-170Yao Qingjun,Jia Chiyu,Chen Bi,et al.A rat model of steam scald establishment.Chinese Journal of Burns,2004;20(3):168-170
8 Toliver-Kinsky T E,Varma T K,Lin C Y,et al.Interferon-gamma production is suppressed in thermally injured mice:Decreased production of regulatory cytokines and corresponding receptors.Shock,2002;18(4):322-330
9刘鹏琪.美容仪器(IPL强脉冲光):201230155984.2.2012-11-07Liu Pengqi.Beauty treatment apparatus(IPL intense pulsed light):201230155984.2.2012-11-07
10岳永恒,刘海霞,秦应雄.一种多功能光电美容仪:201420390131.0.2014-12-17Yue Yongheng,Liu Haixia,Qin Yingxiong.Multifunctional photoelectric beauty instrument:201420390131.0.2014-12-17
2 Negishi K,Wakamatsu S,Kushikata N,et al.Full-face photorejuvenation of photodamaged skin by intense pulsed light with integrated contact cooling:Initial experiences in Asian patients.Lasers in Surgery and Medicine,2002;30(4):298-305
3陈孝平,汪建平.外科学.8版.北京:人民卫生出版社,2013:360-362Chen Xiaoping,Wang Jianping.Surgery.8th ed.Beijing:People's Health Publishing House,2013:360-362
4张大伟,龚震宇,彭毅志.大鼠烫伤创面感染模型的研制.中华烧伤杂志,2011;(2):104-108Zhang Dawei,Gong Zhenyu,Peng Yizhi.Development of rat scald wound infection model.Chinese Journal of Burns,2011;(2):104-108
5刘颖,陈镇,夏泉,等.烧伤膏对烫伤小鼠创面病理修复作用的影响.中国中医急症,2011;20(5):746-748Liu Ying,Chen Zhen,Xia Quan,et al.Effects of burn ointment on pathological repair of wounds in scalded mice.Chinese Journal of Emergency Medicine,2011;20(5):746-748
6曹蔚,崔芬芳,谢艳华,等.椒目仁油合用盐酸小檗碱治疗烫伤的研究.科学技术与工程,2011;11(36):8970-8973Cao Wei,Cui Fenfang,Xie Yanhua,et al.Study on the treatment of scald with berberine hydrochloride combined with Jiao Mu Ren You.Science Technology and Engineering,2011;11(36):8970-8973
7姚庆君,贾赤宇,陈璧,等.一种大鼠蒸气烫伤模型的建立.中华烧伤杂志,2004;(3):168-170Yao Qingjun,Jia Chiyu,Chen Bi,et al.A rat model of steam scald establishment.Chinese Journal of Burns,2004;20(3):168-170
8 Toliver-Kinsky T E,Varma T K,Lin C Y,et al.Interferon-gamma production is suppressed in thermally injured mice:Decreased production of regulatory cytokines and corresponding receptors.Shock,2002;18(4):322-330
9刘鹏琪.美容仪器(IPL强脉冲光):201230155984.2.2012-11-07Liu Pengqi.Beauty treatment apparatus(IPL intense pulsed light):201230155984.2.2012-11-07
10岳永恒,刘海霞,秦应雄.一种多功能光电美容仪:201420390131.0.2014-12-17Yue Yongheng,Liu Haixia,Qin Yingxiong.Multifunctional photoelectric beauty instrument:201420390131.0.2014-12-17