滑菇肽对青春双歧杆菌的益生作用
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摘要
研究滑菇肽对青春双歧杆菌的体外益生作用。将滑菇肽添加到青春双歧杆菌液体培养基中,测定发酵和贮存过程中活菌数变化。结果表明,滑菇肽在添加量为0.5 g/L～4.0 g/L时对青春双歧杆菌生长具有促进作用,发酵12小时后活菌数最高可达1.78×10~9cfu/mL,为对照组的2.88倍(P<0.05)。滑菇肽能够增强青春双歧杆菌对酸性环境的耐受力,4℃冷藏28天后,活菌数仍维持在80%以上。添加1.0 g/L滑菇肽对青春双歧杆菌的促生长作用优于低聚果糖,发酵12小时后活菌数可达1.18×10~9cfu/mL,为对照组的2.04倍(P<0.05)。滑菇肽能够促进青春双歧杆菌生长,作为双歧因子应用于青春双歧杆菌产品具有潜在价值。
The prebiotic effect of Pholiota nameko peptides on Bifidobacterium adolescent in vitro was studied in this paper. Different concentrations of P.nameko peptides were added into the liquid fermentation medium of B.adolescent,and the viable counts during the fermentation and storage process were determined. The results showed that the growth of B.adolescent was promoted when the concentration of P.nameko peptides varied from0.5 g/L to 4.0 g/L,and the highest viable count was 1.78×10~9 cfu/mL after 12 h fermentation,which was 2.88 times as higher as that of the control group(P<0.05). The tolerance of B.adolescent to acid environment was enhanced by addition of P.nameko peptides,and the viable count remained above 80 % after storage at 4 ℃ for28 d. When the addition was 1.0 g/L,the growth promoting effect of P.nameko peptides on B.adolescent was higher than that of fructooligosaccharides,and the final viable count was 1.18 ×10~9 cfu/mL after 12 h fermentation,which was 2.04 times as higher as that of the control group(P<0.05). P.nameko peptides could promote the growth of B.adolescent,and possess potential application value in B.adolescent products as bifidus factors.
The prebiotic effect of Pholiota nameko peptides on Bifidobacterium adolescent in vitro was studied in this paper. Different concentrations of P.nameko peptides were added into the liquid fermentation medium of B.adolescent,and the viable counts during the fermentation and storage process were determined. The results showed that the growth of B.adolescent was promoted when the concentration of P.nameko peptides varied from0.5 g/L to 4.0 g/L,and the highest viable count was 1.78×10~9 cfu/mL after 12 h fermentation,which was 2.88 times as higher as that of the control group(P<0.05). The tolerance of B.adolescent to acid environment was enhanced by addition of P.nameko peptides,and the viable count remained above 80 % after storage at 4 ℃ for28 d. When the addition was 1.0 g/L,the growth promoting effect of P.nameko peptides on B.adolescent was higher than that of fructooligosaccharides,and the final viable count was 1.18 ×10~9 cfu/mL after 12 h fermentation,which was 2.04 times as higher as that of the control group(P<0.05). P.nameko peptides could promote the growth of B.adolescent,and possess potential application value in B.adolescent products as bifidus factors.
引文
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[20]李好,于世军,王伟,等.富硒猴头菇对小鼠肠道菌群的调节作用[J].中国微生态学杂志, 2010, 22(8):697-700
[21] Oyetayo VO, Oyetayo FL. Preliminary study of the health promoting potentials of Lactobacillus fermentum OVL and Plerotus sajor caju administered to rats[J]. Pakistan Journal of Nutrition, 2005, 4(2):73-77
[22] Giannenas I, Tontis D, Tsalie E, et al. Influence of dietary mushroom Agaricus bisporus on intestinal morphology and microflora composition in broiler chickens[J]. Research in Veterinary Science, 2010,89(1):78-84
[23]蔡文辉,杨景云,王亚贤,等.灵芝孢子粉对重症急性胰腺炎大鼠肠道微生态失调的调整作用[J].中国微生态学杂志, 2008, 20(2):140-142
[24]钱磊,张志军,周永斌,等.酶法水解滑菇蛋白制备抗氧化肽的工艺优化[J].食品工业科技, 2017, 38(20):72-76
[25]蔡露阳,张蓓蕾.大米肽对嗜酸乳杆菌的促生长作用[J].食品科技, 2017, 42(3):10-14
[2]兰玉菲,安秀荣,王庆武,等.蕈菌作为益生元新来源的潜力研究[J].中国食用菌, 2011,30(2):52-54
[3]王芳,马俪珍,孙卫青.香菇浸提液对乳酸菌生长的作用[J].山西农业大学学报(自然科学版), 2002, 22(2):159-161
[4]郭红珍,张燕.金针菇与香菇酸奶的研制[J].中国乳业,2005,25(9):37-39
[5]李科德,韩木兰.猴头菇抽提物对乳酸菌生长的影响[J].微生物学杂志, 2000, 20(2):34-35
[6]王世强,屈艳.猴头菇复合保健酸奶的研制[J].中国酿造, 2009,28(10):166-167
[7]张功,王瑞君,峥嵘.平菇浸汁促进嗜酸乳杆菌生长的研究[J].微生物学通报, 2001, 29(4):65-67
[8]韩雪,张兰威.双歧杆菌增殖因子的筛选及培养基的优化[J].食品与生物技术学报, 2005, 24(4):69-72
[9] Synytsya A, Míc姚kováK, Synytsya A, et al. Glucans from fruit bodies of cultivated mushrooms Pleurotus ostreatus and Pleurotus eryngii:Structure and potential prebiotic activity[J]. Carbohydrate Polymers,2009, 76(4):548-556
[10]刘丹,潘道东.柳松菇抽提物对乳酸菌发酵性状的影响[J].中国乳品工业, 2005, 33(12):12-15
[11]曹荣安,韩建春,孔保华,等.巴西菇对嗜热链球菌生长影响的研究[J].食品工业科技, 2006, 27(1):79-80
[12]闫丽莉,韩建春.姬松茸多糖对乳酸菌生长的影响[J].中国乳品工业, 2008, 36(12):7-10
[13]赵伯菁.三种食用菌提取物对乳酸菌生长的影响[J].食品科技,2009, 34(12):261-264
[14]王芳,马俪珍.鸡腿菇浸提液对乳酸菌生长的作用[J].山西农业大学学报(自然科学版), 2003, 23(4):342-344
[15] Gao S, Lai CKM, Cheung CKP. Nondigestible carbohydrates isolated from medicinal mushroom sclerotia as novel prebiotics[J]. International Journal of Medicinal Mushrooms, 2009, 11(1):1-8
[16] Guo FC, Williams BA, Kwakkel RPK, et al. Effects of mushroom and herb polysaccharides, as alternatives for an antibiotic, on the cecal microbial ecosystemin broiler chickens[J]. Poultry Science, 2004, 83(2):175-182
[17] Willis WL, Isikhuemhen OS, Ibrahim SA. Performance assessment of broiler chickens given mushroom extract alone or in conbination with probiotics[J]. Poultry Science, 2007, 86(9):1856-1860
[18] Willis WL, King K, Iskhuemhen OS, et al. Administration of mushroom extract to broiler chickens for bifidobacteria enhancement and Salmonella reduction[J]. Journal of Applied Poultry Research, 2009,18(4):658-664
[19]傅祖良,王康宁,吴仿琪,等.银耳孢子发酵物及与p GRF基因质粒合用对断奶仔猪生长、部分免疫指标及肠道菌群的影响[J].动物营养学报, 2009, 21(5):777-783
[20]李好,于世军,王伟,等.富硒猴头菇对小鼠肠道菌群的调节作用[J].中国微生态学杂志, 2010, 22(8):697-700
[21] Oyetayo VO, Oyetayo FL. Preliminary study of the health promoting potentials of Lactobacillus fermentum OVL and Plerotus sajor caju administered to rats[J]. Pakistan Journal of Nutrition, 2005, 4(2):73-77
[22] Giannenas I, Tontis D, Tsalie E, et al. Influence of dietary mushroom Agaricus bisporus on intestinal morphology and microflora composition in broiler chickens[J]. Research in Veterinary Science, 2010,89(1):78-84
[23]蔡文辉,杨景云,王亚贤,等.灵芝孢子粉对重症急性胰腺炎大鼠肠道微生态失调的调整作用[J].中国微生态学杂志, 2008, 20(2):140-142
[24]钱磊,张志军,周永斌,等.酶法水解滑菇蛋白制备抗氧化肽的工艺优化[J].食品工业科技, 2017, 38(20):72-76
[25]蔡露阳,张蓓蕾.大米肽对嗜酸乳杆菌的促生长作用[J].食品科技, 2017, 42(3):10-14