钴60-γ射线辐射对天花粉有效成分的影响
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摘要
目的:考察钴60(~(60)Co-γ)射线辐照灭菌效果及对天花粉有效成分含量的影响。方法:选择辐照剂量为0,5,8,10kGy对天花粉药材辐照灭菌,采用微生物限度检查法考察灭菌前后需氧菌总数和霉菌、酵母菌总数变化; HPLC法比较有效成分葫芦素B、葫芦素E的含量变化,并检验其显著性情况。结果:天花粉经8 kGy辐照剂量灭菌可达到中国药典微生物限度要求;天花粉中有效成分葫芦素B、葫芦素E的含量在≤8 kGy辐照后无明显变化(P> 0. 05),但经10 kGy辐照后,葫芦素B、葫芦素E含量变化显著(P <0. 05)。结论:辐照剂量为8 kGy时,~(60)Co-γ射线可作为天花粉药材辐照灭菌方法。
Objective: To study the efficacy of sterilization and explore the effect of ~(60)Co-γ ray irradiation on the effective components of Radix Trichosanthis. Methods: The sliced crude materials of Radix Trichosanthis were irradiated by ~(60)Co-γ ray respectively at 0,5,8 and 10 kGy. The changes in the total numbers of aerobic bacteria,molds and yeast before and after the sterilization were investigated by microbiological limit examination and the content changes of the active ingredients cucurbitacch B and cucurbitacch E were detected by HPLC. Results: After 8 kGy radiation,Radix Trichosanthis could meet the microbial limit requirements of Chinese Pharmacopoeia. There were no significant changes in the contents of cucurbitacch B and cucurbitacch E in Radix Trichosanthis after ≤8kGy irradiation (P > 0. 05),but there were significant changes in the contents of cucurbitacch B and cucurbitacch E after 10 kGy radiation (P < 0. 05). Conclusion:~(60)Co-γ ray at 8 kGy can be used as the dosage for irradiation sterilization of Radix Trichosanthis.
Objective: To study the efficacy of sterilization and explore the effect of ~(60)Co-γ ray irradiation on the effective components of Radix Trichosanthis. Methods: The sliced crude materials of Radix Trichosanthis were irradiated by ~(60)Co-γ ray respectively at 0,5,8 and 10 kGy. The changes in the total numbers of aerobic bacteria,molds and yeast before and after the sterilization were investigated by microbiological limit examination and the content changes of the active ingredients cucurbitacch B and cucurbitacch E were detected by HPLC. Results: After 8 kGy radiation,Radix Trichosanthis could meet the microbial limit requirements of Chinese Pharmacopoeia. There were no significant changes in the contents of cucurbitacch B and cucurbitacch E in Radix Trichosanthis after ≤8kGy irradiation (P > 0. 05),but there were significant changes in the contents of cucurbitacch B and cucurbitacch E after 10 kGy radiation (P < 0. 05). Conclusion:~(60)Co-γ ray at 8 kGy can be used as the dosage for irradiation sterilization of Radix Trichosanthis.
引文
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2黄赵刚,陈泳伍,程钢,等.不同剂量钴-60辐照对制痂酊中儿茶素和表儿茶素含量的影响[J].中国药师,2011,14(10):1463-1464
3王二兵,赵正保,曲婷丽.60Co辐照灭菌对止嗽立效口服液主要成分的影响[J].中成药,2013,35(9):2056-2058
4中国药典[S].2015年版.一部.280-281
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6张雅男,高明洁,祝文博,等.HPLC-UV法同时测定葫芦素片中葫芦素B和葫芦素E的含量[J].化学工程师,2018,32(3):18-20
7谭忠谋.5种葫芦科植物中葫芦素类成分的含量测定及灰色关联度分析[J].中国药师,2017,20(7):1201-1205
8杨异卉,陈卫军,甘春丽,等.戒酒灵口服液中葫芦素B、葫芦素E的TLC鉴别和HPLC含量测定[J].哈尔滨医科大学学报,2015,49(2):95-98+102
9黄庆勇,李煌,许文,等.君药天花粉对复方栝楼桂枝汤中9种成分的影响[J].中国实验方剂学杂志,2014,20(12):57-60
10雷曦,申鸿.60Co-γ射线辐照贮藏中药材对其主要成分的影响[J].中国药房,2002,13(2):119-120
2黄赵刚,陈泳伍,程钢,等.不同剂量钴-60辐照对制痂酊中儿茶素和表儿茶素含量的影响[J].中国药师,2011,14(10):1463-1464
3王二兵,赵正保,曲婷丽.60Co辐照灭菌对止嗽立效口服液主要成分的影响[J].中成药,2013,35(9):2056-2058
4中国药典[S].2015年版.一部.280-281
5李晓峰,刘克非,邓意辉.HPLC法同时测定葫芦素原料中葫芦素B和葫芦素E的含量[J].沈阳药科大学学报,2010,27(2):123-125,130
6张雅男,高明洁,祝文博,等.HPLC-UV法同时测定葫芦素片中葫芦素B和葫芦素E的含量[J].化学工程师,2018,32(3):18-20
7谭忠谋.5种葫芦科植物中葫芦素类成分的含量测定及灰色关联度分析[J].中国药师,2017,20(7):1201-1205
8杨异卉,陈卫军,甘春丽,等.戒酒灵口服液中葫芦素B、葫芦素E的TLC鉴别和HPLC含量测定[J].哈尔滨医科大学学报,2015,49(2):95-98+102
9黄庆勇,李煌,许文,等.君药天花粉对复方栝楼桂枝汤中9种成分的影响[J].中国实验方剂学杂志,2014,20(12):57-60
10雷曦,申鸿.60Co-γ射线辐照贮藏中药材对其主要成分的影响[J].中国药房,2002,13(2):119-120