两种植物根际促生菌对博落回抗干旱及富集铀性能的增强作用研究
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摘要
采用土壤盆栽试验,通过在博落回(Macleaya cordata)根际单独接种荧光假单胞菌(Pseudomonas fluorescens)、单独接种绿针假单胞菌橙色亚种(Pseudomonas chlororaphis subsp. aurantiaca)及同时接种这两种菌,研究了这两种植物根际促生菌对博落回抗干旱及富集铀性能的增强作用.结果表明:在干旱胁迫下,单独接种荧光假单胞菌、单独接种绿针假单胞菌橙色亚种及同时接种这两种菌后,博落回的生物量分别增加了35.03%、21.01%和17.33%,相对含水量分别增加了26.90%、19.31%和29.48%,叶绿素总量分别增加了71.11%、48.47%、12.85%,脯氨酸含量分别降低了71.70%、64.91%和61.35%.在干旱和铀同时胁迫下,单独接种荧光假单胞菌、单独接种绿针假单胞菌橙色亚种及同时接种这两种菌后,博落回对铀的总富集量分别提高了109.52%、66.67%和42.86%.可见,两种植物根际促生菌对博落回抗干旱和富集铀性能的增强作用表现为:单独接种荧光假单胞菌>单独接种绿针假单胞菌橙色亚种>同时接种这两种菌.实验结果可为干旱地区铀污染土壤的植物修复提供参考.
Pot experiments were conducted to investigate the enhancement on drought resistance and uranium bioaccumulation abilities of Macleaya cordata inoculated with Pseudomonas fluorescens,Pseudomonas chlororaphis subsp. aurantiaca and both of them,respectively. It was found that,under drought stress,the biomasses of Macleaya cordata inoculated with Pseudomonas fluorescens,Pseudomonas chlororaphis subsp. aurantiaca and both of them,respectively increased by 35.03%,21.01% and 17.33%,respectively; the relative water contents increased by 26.90%,19.31% and 29.48%,respectively; the total chlorophyll contents increased by 71. 11%,48. 47% and 12. 85%,respectively; but the proline contents reduced by 71. 70%,64.91% and 61.35%,respectively. Under both uranium and drought stresses,the total bioaccumulation amounts of uranium in Macleaya cordata increased by 109.52%,66.67% and 42.86%,respectively. The results show that the drought resistance and uranium bioaccumulation abilities of Macleaya cordata inoculated with Pseudomonas fluorescens,Pseudomonas chlororaphis subsp. aurantiaca and both of them,respectively were in the order of Pseudomonas fluorescens > Pseudomonas chlororaphis subsp. aurantiaca > both strains. The results can provide reference for phytoremediation of uranium contaminated soil in arid regions.
Pot experiments were conducted to investigate the enhancement on drought resistance and uranium bioaccumulation abilities of Macleaya cordata inoculated with Pseudomonas fluorescens,Pseudomonas chlororaphis subsp. aurantiaca and both of them,respectively. It was found that,under drought stress,the biomasses of Macleaya cordata inoculated with Pseudomonas fluorescens,Pseudomonas chlororaphis subsp. aurantiaca and both of them,respectively increased by 35.03%,21.01% and 17.33%,respectively; the relative water contents increased by 26.90%,19.31% and 29.48%,respectively; the total chlorophyll contents increased by 71. 11%,48. 47% and 12. 85%,respectively; but the proline contents reduced by 71. 70%,64.91% and 61.35%,respectively. Under both uranium and drought stresses,the total bioaccumulation amounts of uranium in Macleaya cordata increased by 109.52%,66.67% and 42.86%,respectively. The results show that the drought resistance and uranium bioaccumulation abilities of Macleaya cordata inoculated with Pseudomonas fluorescens,Pseudomonas chlororaphis subsp. aurantiaca and both of them,respectively were in the order of Pseudomonas fluorescens > Pseudomonas chlororaphis subsp. aurantiaca > both strains. The results can provide reference for phytoremediation of uranium contaminated soil in arid regions.
引文
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Garbisu C,Alkorta I.2001.Phytoextraction:a cost-effective plant-based technology for the removal of metals from the environment[J].Bioresource Technology,77(3):229-236
Glick B R,Penrose D M,Li J.1998.A model for the lowering of plant ethylene concentrations by plant growth-promoting bacteria[J].Journal of Theoretical Biology,190(1):63-68
Hayat S,Hayat Q,Alyemeni M N,et al.2012.Role of proline under changing environments:a review[J].Plant Signaling&Behavior,7(11):1456-1466
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Jian N,Liu Y,Zeng G,et al.,2016.Cadmium accumulation and tolerance of Macleaya cordata:a newly potential plant for sustainable phytoremediation in Cd-contaminated soil[J].Environmental Science and Pollution Research International,23(10):10189-10199
Jin R,Shi H,Han C,et al.2015.Physiological changes of purslane(Portulaca oleracea L.)after progressive drought stress and rehydration[J].Scientia Horticulturae,194:215-221
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Kuppusamy S,Palanisami T,Megharaj M,et al.2016.In-situ remediation approaches for the management of contaminated sites:a comprehensive overview[J].Rev Environ Contam Toxicol,236(18):1-115.
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李合生.2006.植物生理生化实验原理和技术[M].北京:高等教育出版社.192-194
Ma Y,Rajkumar M,Freitas H.2009.Isolation and characterization of Ni mobilizing PGPB from serpentine soils and their potential in promoting plant growth and Ni accumulation by Brassica spp[J].Chemosphere,75(6):719-725
Ma Y,Prasad M N,RajkumarM,et al.2011.Plant growth promoting rhizobacteria and endophytes accelerate phytoremediation of metalliferous soils[J].Biotechnology Advances,29(2):248-258
Ma Y,Rajkumar M,Chang Z,et al.2016.Inoculation of Brassica oxyrrhina,with plant growth promoting bacteria for the improvement of heavy metal phytoremediation under drought conditions[J].Journal of Hazardous Materials,320:36-44
Ortiz N,Armada E,Duque E,et al.2015.Contribution of arbuscular mycorrhizal fungi and/or bacteria to enhancing plant drought tolerance under natural soil conditions:Effectiveness of autochthonous or allochthonous strains[J].Journal of Plant Physiology,174:87-96
Pane C,Zaccardelli M.2015.Evaluation of Bacillus strains isolated from solanaceous phylloplane for biocontrol of Alternaria early blight of tomato[J].Biological Control,84:11-18
Patten C L,Glick B R.2002.Role of Pseudomonas putida indoleacetic acid in development of the host plant root system[J].Applied and Environmental Microbiology,68(8):3795-3801
Rajkumar M,Ae N,Prasad M N V,et al.2010.Potential of siderophoreproducing bacteria for improving heavy metal phytoextraction[J].Trends in Biotechnology,28(3):142-149
Sheng X F,Xia J J.2006.Improvement of rape(Brassica napus)plant growth and cadmium uptake by cadmium-resistant bacteria[J].Chemosphere,64(6):1036-1042
Siddikee M A,Glick B R,Chauhan P S,et al.2011.Enhancement of growth and salt tolerance of red pepper seedlings(Capsicum annuum L.)by regulating stress ethylene synthesis with halotolerantbacteriacontaining1-aminocyclopropane-1-carboxylicaciddeaminase activity[J].Plant Physiology&Biochemistry,49(4):427-434
Soudek P,Petrova S,Buzek M,et al.2014.Uranium uptake in Nicotiana sp under hydroponic conditions[J].Journal of Geochem Explor,142(4):130-137
谭煜健,胡南,张辉,等.2017.满江红对不同形态铀的吸附行为[J].环境科学学报,37(10):3713-3719
Venkatesh N M,Vedaraman N.2012.Remediation of soil contaminated with copper using Rhamnolipids produced from Pseudomonas aeruginosa,MTCC 2297 using waste frying rice bran oil[J].Annals of Microbiology,62(1):85-91
Vurukonda S S,Vardharajula S,Shrivastava M,et al.2016.Enhancement of drought stress tolerance in crops by plant growth promoting rhizobacteria[J].Microbiological Research,184:13-24
谢文华,陈琴,白璐.2017.干旱胁迫下3种驱蚊植物营养生长期叶绿素含量变化[J].中国测试,35(6I):185-193
余林岚,程辟,唐其,等.2017.不同胁迫博落回根中生物碱含量变化及相关基因表达量分析[J].分子植物育种,15(4):1-7
张甘霖,龚子同.2012.土壤调查实验室分析方法[M].北京:科学出版社
赵青云,赵秋芳,王辉,等.2015.根际促生菌Bacillus subtilisY-IVI在香草兰上的应用效果研究[J].植物营养与肥料学报,21(2):535-540
朱春云,刘霞.1996.锦鸡儿等旱生树种抗旱生理的研究[J].干旱区研究,(1):59-63
Armada E,Azcon R,Lopez-Castillo O M,et al.2015.Autochthonous arbuscular mycorrhizal fungi and Bacillus thuringiensis from a degraded Mediterranean area can be used to improve physiological traits and performance of a plant of agronomic interest under drought conditions[J].Plant Physiology and Biochemistry,90:64-74
Ashraf M,Harris P J C.2013.Photosynthesis under stressful environments:An overview[J].Photosynthetica,51(2):163-190
Benabdellah K,Abbas Y,Abourouh M,et al.2011.Influence of two bacterial isolates from degraded and non-degraded soils and arbuscular mycorrhizae fungi isolated from semi-arid zone on the growth of Trifolium repens under drought conditions:Mechanisms related tobacterial effectiveness[J].European Journal of Soil Biology,47(5):303-309
Braud A,Jezequel K,Bazot S,et al.2009.Enhanced phytoextraction of an agricultural Cr-and Pb-contaminated soil by bioaugmentation with siderophore-producingbacteria[J].Chemosphere,74(2):280-286
Carlier E,Rovera M,Jaume A R,et al.2008.Improvement of growth,under field conditions,of wheat inoculated with Pseudomonas chlororaphis,subsp.aurantiaca,SR1[J].World Journal of Microbiology&Biotechnology,24(11):2653-2658
Chaney R L,Angle J S,Broadhurst C L,et al.2007.Improved understanding of hyperaccumulation yields commercial phytoextraction and phytomining technologies[J].Journal of Environmental Quality,36(5):1429-1443
Chaves M M,Flexas J,Pinheiro C.2009.Photosynthesis under drought and salt stress:regulation mechanisms from whole plant to cell[J].Annals of Botany,103(4):551-560
Colom M R,Vazzana C.2003.Photosynthesis and PSII functionality of drought-resistant and drought-sensitive weeping lovegrass plants[J].Environmental and Experimental Botany,49(2):135-144
丁德馨,李广悦,胡南,等.2010.一种利用植物修复铀尾矿砂的方法[P].中国专利.CN101642768.2010-02-10
Ding Y J,Zhang J J.2016.Extraction of REPs from leaves reflectance spectrum for estimation of chlorophyll content[J].International Federation of Automatic Control-Papers On Line,49(16):205-208
Dimkpa C O,Merten D,Svato Scaron A,et al.2009.Metal-induced oxidative stress impacting plant growth in contaminated soil is alleviated by microbial siderophores[J].Soil Biology&Biochemistry,41(1):154-162
Garbisu C,Alkorta I.2001.Phytoextraction:a cost-effective plant-based technology for the removal of metals from the environment[J].Bioresource Technology,77(3):229-236
Glick B R,Penrose D M,Li J.1998.A model for the lowering of plant ethylene concentrations by plant growth-promoting bacteria[J].Journal of Theoretical Biology,190(1):63-68
Hayat S,Hayat Q,Alyemeni M N,et al.2012.Role of proline under changing environments:a review[J].Plant Signaling&Behavior,7(11):1456-1466
胡南,丁德馨,李广悦,等.2012.五种水生植物对水中铀的去除作用[J].环境科学学报,33(7):1637-1645
Ings J,Mur L A J,Robson P R H,et al.2013.Physiological and growth responses to water deficit in the bioenergy crop Miscanthus x giganteus[J].Frontiers in Plant Science,4(2):468
Jian N,Liu Y,Zeng G,et al.,2016.Cadmium accumulation and tolerance of Macleaya cordata:a newly potential plant for sustainable phytoremediation in Cd-contaminated soil[J].Environmental Science and Pollution Research International,23(10):10189-10199
Jin R,Shi H,Han C,et al.2015.Physiological changes of purslane(Portulaca oleracea L.)after progressive drought stress and rehydration[J].Scientia Horticulturae,194:215-221
Kamnev A A,Lelie D V D.2000.Chemical and biological parameters as tools to evaluate and improve heavy metal phytoremediation[J].Bioscience Reports,20(4):239-258
Khoyerdi F F,Shamshiri M H,Estaji A.2016.Changes in some physiological and osmotic parameters of several pistachio genotypes under drought stress[J].Scientia Horticulturae,198:44-51
Kuppusamy S,Palanisami T,Megharaj M,et al.2016.In-situ remediation approaches for the management of contaminated sites:a comprehensive overview[J].Rev Environ Contam Toxicol,236(18):1-115.
Li C W,Hu N,Ding D X,et al.2015.Phytoextraction of uranium from contaminated soil by Macleaya cordata before and after application of EDDS and CA[J].Environmental Science&Pollution Research,22(8):6155-6163
李旭,韦靖,欧杨丽桃,等.2015.不同有色金属矿区的三种植物中五种重金属元素含量的测定[J].福建分析测试,24(5):18-22
李合生.2006.植物生理生化实验原理和技术[M].北京:高等教育出版社.192-194
Ma Y,Rajkumar M,Freitas H.2009.Isolation and characterization of Ni mobilizing PGPB from serpentine soils and their potential in promoting plant growth and Ni accumulation by Brassica spp[J].Chemosphere,75(6):719-725
Ma Y,Prasad M N,RajkumarM,et al.2011.Plant growth promoting rhizobacteria and endophytes accelerate phytoremediation of metalliferous soils[J].Biotechnology Advances,29(2):248-258
Ma Y,Rajkumar M,Chang Z,et al.2016.Inoculation of Brassica oxyrrhina,with plant growth promoting bacteria for the improvement of heavy metal phytoremediation under drought conditions[J].Journal of Hazardous Materials,320:36-44
Ortiz N,Armada E,Duque E,et al.2015.Contribution of arbuscular mycorrhizal fungi and/or bacteria to enhancing plant drought tolerance under natural soil conditions:Effectiveness of autochthonous or allochthonous strains[J].Journal of Plant Physiology,174:87-96
Pane C,Zaccardelli M.2015.Evaluation of Bacillus strains isolated from solanaceous phylloplane for biocontrol of Alternaria early blight of tomato[J].Biological Control,84:11-18
Patten C L,Glick B R.2002.Role of Pseudomonas putida indoleacetic acid in development of the host plant root system[J].Applied and Environmental Microbiology,68(8):3795-3801
Rajkumar M,Ae N,Prasad M N V,et al.2010.Potential of siderophoreproducing bacteria for improving heavy metal phytoextraction[J].Trends in Biotechnology,28(3):142-149
Sheng X F,Xia J J.2006.Improvement of rape(Brassica napus)plant growth and cadmium uptake by cadmium-resistant bacteria[J].Chemosphere,64(6):1036-1042
Siddikee M A,Glick B R,Chauhan P S,et al.2011.Enhancement of growth and salt tolerance of red pepper seedlings(Capsicum annuum L.)by regulating stress ethylene synthesis with halotolerantbacteriacontaining1-aminocyclopropane-1-carboxylicaciddeaminase activity[J].Plant Physiology&Biochemistry,49(4):427-434
Soudek P,Petrova S,Buzek M,et al.2014.Uranium uptake in Nicotiana sp under hydroponic conditions[J].Journal of Geochem Explor,142(4):130-137
谭煜健,胡南,张辉,等.2017.满江红对不同形态铀的吸附行为[J].环境科学学报,37(10):3713-3719
Venkatesh N M,Vedaraman N.2012.Remediation of soil contaminated with copper using Rhamnolipids produced from Pseudomonas aeruginosa,MTCC 2297 using waste frying rice bran oil[J].Annals of Microbiology,62(1):85-91
Vurukonda S S,Vardharajula S,Shrivastava M,et al.2016.Enhancement of drought stress tolerance in crops by plant growth promoting rhizobacteria[J].Microbiological Research,184:13-24
谢文华,陈琴,白璐.2017.干旱胁迫下3种驱蚊植物营养生长期叶绿素含量变化[J].中国测试,35(6I):185-193
余林岚,程辟,唐其,等.2017.不同胁迫博落回根中生物碱含量变化及相关基因表达量分析[J].分子植物育种,15(4):1-7
张甘霖,龚子同.2012.土壤调查实验室分析方法[M].北京:科学出版社
赵青云,赵秋芳,王辉,等.2015.根际促生菌Bacillus subtilisY-IVI在香草兰上的应用效果研究[J].植物营养与肥料学报,21(2):535-540
朱春云,刘霞.1996.锦鸡儿等旱生树种抗旱生理的研究[J].干旱区研究,(1):59-63