基因重组人生长激素复合纳米羟基磷灰石对兔胫骨骨缺损的修复效果
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摘要
目的:观察基因重组人生长激素(rhGH)复合纳米羟基磷灰石(nHA)修复兔胫骨缺损过程中的成骨效果,以探讨两种药物联合使用在骨缺损修复中的作用。方法:新西兰大白兔24只,于双侧胫骨制备骨缺损模型;随机分为4组:空白对照组、rhGH/nHA组、rhGH组、nHA组,并行相应处理。分别于术后第1、2和4周进行骨缺损大体观察、X线影像学、组织学检测以及新生骨占缺损面积百分比的测定。结果:各组都有不同程度的骨组织形成,在术后第1、2和4周,各组之间新生骨组织面积百分比的差异均存在统计学意义(P<0.05)。纵向比较,除空白组外,其他组在第1、2、4周之间新生成骨面积百分比的差异均存在统计学意义(P<0.05)。结论:局部应用基因重组人生长激素复合纳米羟基磷灰石可以促进骨缺损区新生骨组织生成,且效果优于分别单独使用两种材料。
Objective:To evaluate the effect of the topical application of recombinant human growth hormon(GH)and nano-hydroxyapatite on repair of bone defects in the tibia of rabbits model.Methods:The bilateral tibia defects models were established in twenty-four New Zealand rabbits.They were divided into four groups randomly:rhGH/nHA Composite Group,rhGH Group,nHA Group,and control Group.Every group has been treated with corresponding treatment.General observation,X-ray radiograph,histological examination and the osteogenesis effect in the defect region were analyzed after 1,2and 4weeks.Results:In each postoperative time point,each group had varying degree of new bone formation.There were significant differences of the quantity of new bone area among each group in 1,2,and 4weeks after the treatment(P<0.05).Longitudinal comparison showed that there were significant differences of the quantity of new bone area among each group at each time points(P<0.05),except for the control group.Conclusion:Topical application of rhGH and nHA can accerlerate the new bone formation in bone defect area.The osteogenesis effect of the combination use of these two materials is better than using them separately.
Objective:To evaluate the effect of the topical application of recombinant human growth hormon(GH)and nano-hydroxyapatite on repair of bone defects in the tibia of rabbits model.Methods:The bilateral tibia defects models were established in twenty-four New Zealand rabbits.They were divided into four groups randomly:rhGH/nHA Composite Group,rhGH Group,nHA Group,and control Group.Every group has been treated with corresponding treatment.General observation,X-ray radiograph,histological examination and the osteogenesis effect in the defect region were analyzed after 1,2and 4weeks.Results:In each postoperative time point,each group had varying degree of new bone formation.There were significant differences of the quantity of new bone area among each group in 1,2,and 4weeks after the treatment(P<0.05).Longitudinal comparison showed that there were significant differences of the quantity of new bone area among each group at each time points(P<0.05),except for the control group.Conclusion:Topical application of rhGH and nHA can accerlerate the new bone formation in bone defect area.The osteogenesis effect of the combination use of these two materials is better than using them separately.
引文
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[2]Andersson B,Swolin D,Magnusson P,et al.Shortterm changes in bone formation markers following growth h-ormone(GH)treatment in short prepubertal children with a broad range of GH secretion[J].Clin Endocrinol,2015,82(1):91-99.
[3]Thorsted A,Thygesen P,Agerso H,et al.Translational mixed-effects PKPD modelling of recombin-ant human growth hormone-from hypophysectomized rat to patients[J].British Journal of Pharmacology,2016,173(11):1 742-1 755.
[4]Vestergaarda B,Thygesen P,Kreilgaard M,et al.The kidneys play a central role in the clearance of rhGH in rats[J].European Journal of Pharmaceutical Sciences,2016,86(30):29-33.
[5]Krai K,Apichart L,Chamnan R,et al.Influence of the nano hydroxyapatite powder on thermally sprayed HA coatings onto stainless steel[J].Surface&Coatings Technology,2016,306(25):181-186.
[6]Hu YJ,Liu WT,Wang YL,et al.Effects of recombinant human growth hormone on local expression of insulinlike growth factor-Ⅰduring orthodontic tooth movement[J].Chinese journal of stomatology,2016,51(6):374-378.
[7]Hevroy EM,Hunskar C.GH-IGF system regulation of attenuated muscl-e growth and lipolysis in Atlantic salmon reared at elevated sea temperatures[J].Journal of Comparative Physiology B,2013,183(2):243-259.
[8]Miletta MC,Fluck CE,Mullis PE.Targeting GH-1splicing as a novel phar macological strategy for growth hormone cleficiency typeⅡ[J].Biochenmical Pharmacology,2017,124(1):1-9.
[9]Mrak E,Casati L,Pagani F,et al.Ghrelin increases beta-catenin level through protein kinase a activation and regulates OPG expression in rat primary osteoblasts[J].International Journal of Endocrinology.2015,12(21):1-10.
[10]Labarta JI,Barrio E,AudíL,et al.Familial short stature and intrauterine growth retardation associated with a novel mutation in the IGF-Ⅰreceptor gene[J].Clinical Endocrinology,2013,78(2):255-262.
[11]Tian QM,Castaneda LR,Liu H,et al.Optimization of nano-hydroxyapatite/poly(lactic-co-glycolic acid)coatings on magnesium substrates using one-step electrophoretic deposition[J].Materials Letters,2016,186(1):12-16.
[12]Zhao XX,Ng S,Heng BC,et al.Cytotoxicity of hydroxyapatite nanoparticles is shap-e and cell dependent[J].Arch Toxicol,2013,87(5):1 037-1 052.
[13]Saeed H,Nader N.In vitro biocompatibility of chitosan/hyaluronic acid-containing calcium phosphate bone cements[J].Bioprocess and Biosystems Engineering,2014,37(8):1 507-1 516.
[14]Lee JS,Sohn JY,Lim HC,et al.Tresguerres.Local administration of growth hormone enhances periimplant bonereaction in an osteoporotic rabbit model[J].Journal of Biomedical Materials Research,2016,104(6):1 202-1 209.