微小小单胞菌在慢性根尖周炎感染根管中的定植研究
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摘要
目的研究微小小单胞菌在慢性根尖周炎患者感染根管中的定植情况。方法选取医院自2015年6月-2017年5月收治的160例慢性根尖周炎患者,根据是否首次感染分为首次感染组与再次感染组,采用聚合酶链式反应(PCR)进行微小小单胞菌细菌鉴定,对比两组微小小单胞菌在感染根管中的定植情况,并对比两组微小小单胞菌DNA表达量。结果 200颗患牙中共检出微小小单胞菌阳性57颗,检出率为28.5%,其中首次感染组检出率为46.0%,高于再次感染组的11.0%(P<0.001);首次感染组微小小单胞菌DNA表达量为(0.033±0.021),高于再次感染组DNA表达量(0.021±0.019)(P<0.001)。结论微小小单胞菌在慢性根尖周炎患者感染根管中的定植情况较为普遍,且在首次感染患者中的定植率及DNA表达量均明显高于再次感染患者。
OBJECTIVE To study the colonization of P.micrain infected root canals in patients with chronic apical periodontitis.METHODS A total of 160 patients with chronic apical periodontitis admitted to the hospital from Jun.2015 to May 2017 were selected and divided into the primary infection group and the re-infection group according to whether they were infected for the first time.Polymerase chain reaction(PCR)was used for identification of P.micra.The colonization of P.micrain the infected root canal was compared between the two groups,and the DNA expression levels of P.micra in the two groups was also compared.RESULTS A total of 57 teeth were P.micra positive among the 200 infected teeth,and the detection rate was 28.5%.The detection rate of the primary infection group was 46.0%,which was significantly higher than that of the re-infection group(11.0%,P<0.001).The DNA expression level of P.micrain the primary infection group was(0.033±0.021),which was significantly higher than that in the re-infection group(0.021±0.019)(P<0.001).CONCLUSION The colonization of P.micrain infected root canals is common in patients with chronic apical periodontitis,and the colonization rate and DNA expression level in patients with primary infection are significantly higher than those in re-infected patients.
OBJECTIVE To study the colonization of P.micrain infected root canals in patients with chronic apical periodontitis.METHODS A total of 160 patients with chronic apical periodontitis admitted to the hospital from Jun.2015 to May 2017 were selected and divided into the primary infection group and the re-infection group according to whether they were infected for the first time.Polymerase chain reaction(PCR)was used for identification of P.micra.The colonization of P.micrain the infected root canal was compared between the two groups,and the DNA expression levels of P.micra in the two groups was also compared.RESULTS A total of 57 teeth were P.micra positive among the 200 infected teeth,and the detection rate was 28.5%.The detection rate of the primary infection group was 46.0%,which was significantly higher than that of the re-infection group(11.0%,P<0.001).The DNA expression level of P.micrain the primary infection group was(0.033±0.021),which was significantly higher than that in the re-infection group(0.021±0.019)(P<0.001).CONCLUSION The colonization of P.micrain infected root canals is common in patients with chronic apical periodontitis,and the colonization rate and DNA expression level in patients with primary infection are significantly higher than those in re-infected patients.
引文
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[15]彭源,孙琦.儿童乳牙根尖周炎感染的根管菌丛分析的实验研究[J].实验与检验医学,2018,36(2):174-177.
[16]孙玉亮,曹宏飞,赵今,等.根管治疗一次法和两次法清除感染根管内细菌能力的比较研究[J].口腔医学,2018,38(2):143-145,153.
[17]姚雪芹,侯本祥,张琛.持续性根尖周炎中牙龈卟啉单胞菌的分离与鉴定[J].中华老年口腔医学杂志,2016,14(2):77-82.
[18]孙秀玲,符之熙,陈海贝,等.根管治疗根尖周炎感染患者的效果分析[J].中华医院感染学杂志,2016,26(3):654-655,673.
[19]翟晓存,丁秀琴,邹宏梅,等.一次法与多次法根管治疗慢性根尖周炎的疗效比较[J].中国基层医药,2014,21(2):283-284.
[20]Nibali L,Tomlins P,Akcal A.Radiographic morphology of intrabony defects in the first molars of patients with localized aggressive periodontitis:comparison with health and chronic periodontitis[J].J Periodontal Res,2018,53(4):582-588.
[2]黄希,葛自立,刘健金,等.慢性根尖周炎感染根管的病原学分析及影响因素研究[J].中华医院感染学杂志,2018,28(1):114-117.
[3]孙文娟,黄南楠,杨雨虹,等.截根术治疗下颌磨牙病变疗效观察[J].中华实用诊断与治疗杂志,2015,29(12):1182-1183,1187.
[4]张文,陈玉荣,何谨,等.微小小单胞菌与牙髓卟啉单胞菌在慢性根尖周炎感染根管中定植的关系研究[J].中华医院感染学杂志,2016,26(1):147-149.
[5]樊明文.牙体牙髓病学[M].4版.北京:人民卫生出版社,2012:227-231.
[6]Cogulu D,Uzel A,Oncag O,et al.PCR-based identification of selected pathogens associated with endodontic infections in deciduous and permanent teeth[J].Oral Surg Oral Med Oral Pathol Oral Radiol Endod,2008,106(3):443-449.
[7]AlShwaimi E,Berggreen E,Furusho H,et al.IL-17receptor A signaling is protective in infection-stimulated periapical bone destruction[J].J Immunol,2013,191(4):1785-1791.
[8]赵西珍.微小小单胞菌在慢性根尖周炎感染根管中的定植研究[J].中国实验诊断学,2017,21(11):1920-1922.
[9]纪海,李红,侯本祥.微小小单胞菌与慢性根尖周炎临床症状的相关性研究[J].北京口腔医学,2016,24(1):17-20.
[10]Thawai C,Kittiwongwattana C,Thanaboripat D,et al.Micromonospora soli sp.nov.,isolated from rice rhizosphere soil[J].Antonie Van Leeuwenhoek,2016,109(3):449-456.
[11]Carro L,Riesco R,Sproer C,et al.Micromonospora luteifusca sp.nov.isolated from cultivated Pisum sativum[J].Syst Appl Microbiol,2016,39(4):237-242.
[12]Phongsopitanun W,Kudo T,Ohkuma M,et al.Micromonospora sediminis sp.nov.isolated from mangrove sediment[J].Int J Syst Evol Microbiol,2016,66(8):3235-3240.
[13]Trabelsi I,Oves D,Manteca A,et al.Antimicrobial activities of some actinomycetes isolated from different rhizospheric soils in Tunisia[J].Curr Microbiol,2016,73(2):220-227.
[14]Take A,Nakashima T,Inahashi Y,et al.Analyses of the cell-wall peptidoglycan structures in three genera Micromonospora,Catenuloplanes,and Couchioplanes belonging to the family Micromonosporaceae by derivatization with FDLA and PMP using LC/MS[J].J Gen Appl Microbiol,2016,62(4):199-205.
[15]彭源,孙琦.儿童乳牙根尖周炎感染的根管菌丛分析的实验研究[J].实验与检验医学,2018,36(2):174-177.
[16]孙玉亮,曹宏飞,赵今,等.根管治疗一次法和两次法清除感染根管内细菌能力的比较研究[J].口腔医学,2018,38(2):143-145,153.
[17]姚雪芹,侯本祥,张琛.持续性根尖周炎中牙龈卟啉单胞菌的分离与鉴定[J].中华老年口腔医学杂志,2016,14(2):77-82.
[18]孙秀玲,符之熙,陈海贝,等.根管治疗根尖周炎感染患者的效果分析[J].中华医院感染学杂志,2016,26(3):654-655,673.
[19]翟晓存,丁秀琴,邹宏梅,等.一次法与多次法根管治疗慢性根尖周炎的疗效比较[J].中国基层医药,2014,21(2):283-284.
[20]Nibali L,Tomlins P,Akcal A.Radiographic morphology of intrabony defects in the first molars of patients with localized aggressive periodontitis:comparison with health and chronic periodontitis[J].J Periodontal Res,2018,53(4):582-588.