基因重组人生长激素对兔胫骨骨缺损修复效果的影响
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摘要
目的:观察兔胫骨骨缺损修复过程中应用基因重组人生长激素的促成骨效果,探讨其在骨缺损愈合中的作用。方法:新西兰大白兔15只(4~6月大,2.5~3.5 kg,雌雄不限),于双侧胫骨制备直径3 mm,深6 mm的骨缺损模型;右侧为实验组,在胫骨缺损区注入包含1 IU基因重组人生长激素自体血凝块0.1 m L;左侧为对照组,在胫骨缺损区注入等量自体血凝块。分别于术后2、4和8周进行骨缺损大体观察、X线影像学、组织学检测以及新生骨占缺损面积百分比的测定。结果:术后各时间点的大体观察、X线影像学、组织学切片观察显示,实验组和对照组均有不同程度的新骨形成;在术后2周和4周各组新生骨密度低于正常骨组织,实验组新生骨面积百分比均大于对照组(P<0.05)。术后8周时,实验组缺损表面可见连续的皮质骨形成,植骨区新骨形成并趋于成熟,对照组修复效果次之;两组新生骨面积百分比无显著性差异(P>0.05)。结论:在兔胫骨缺损修复中,基因重组人生长激素能够加速骨缺损区骨小梁的形成和成熟,并对新骨形成有一定促进作用。
Objective:The purpose of the study was to observe the effect of topical use of recombinant human growth hormone(rh GH) in the repair process of tibial bone defects in rabbits and explore the role of rh GH in bone healing.Method:The bilateral tibial defects models(3 mm in diameter,and l 0 mm in depth)were established in 15 New Zealand rabbits(4~6 months old,2.5~3.5 kg,no gender limitations). The right side which treated with1 IU rh GH involved in 0.1 m L autologus blood clot was experimental group,and the left side which treated with 0.1 m L autologus blood clot was control group. In postoperative 2nd,4th,8th week,general observation,X-ray radiograph,histological examination,the osteogenesis effect in the defect region were analyzed. Result:In each postoperative time point,the experimental and control groups showed new bone formation. At postoperative 2nd and 4th week,the densities of the new bones were lower than normal bones,the quantities of new bones were larger in the experimental groups than in the control groups(P <0.05).At postoperative 8th week,continuous cortical bone formations were seen on the surfaces in the experimental group,new bone formed and tended to be mature,but those in the control group were not as good. There was no significant difference between the two groups in term of quantity of new bone area. Conclusion:These findings indicate that the topical use of rh GH could accelerate the formation and maturation of trabecular bone and have some effect on new bone information.
Objective:The purpose of the study was to observe the effect of topical use of recombinant human growth hormone(rh GH) in the repair process of tibial bone defects in rabbits and explore the role of rh GH in bone healing.Method:The bilateral tibial defects models(3 mm in diameter,and l 0 mm in depth)were established in 15 New Zealand rabbits(4~6 months old,2.5~3.5 kg,no gender limitations). The right side which treated with1 IU rh GH involved in 0.1 m L autologus blood clot was experimental group,and the left side which treated with 0.1 m L autologus blood clot was control group. In postoperative 2nd,4th,8th week,general observation,X-ray radiograph,histological examination,the osteogenesis effect in the defect region were analyzed. Result:In each postoperative time point,the experimental and control groups showed new bone formation. At postoperative 2nd and 4th week,the densities of the new bones were lower than normal bones,the quantities of new bones were larger in the experimental groups than in the control groups(P <0.05).At postoperative 8th week,continuous cortical bone formations were seen on the surfaces in the experimental group,new bone formed and tended to be mature,but those in the control group were not as good. There was no significant difference between the two groups in term of quantity of new bone area. Conclusion:These findings indicate that the topical use of rh GH could accelerate the formation and maturation of trabecular bone and have some effect on new bone information.
引文
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[6]Chia DJ.Minireview:Mechanisms of growth hormone-mediated gene regulation[J].Molecular Endorcrinology,2014,28(7):1012-1025.
[7]Calvo-Guirado JL,Mate-Sanchez J.Effects of growth hormone on initial bone formation around dental implants:a dog study[J].Clinical Oral Implants Research,2011,22(6):587-593.
[8]Tresguerres IF,Clemente C.Local administration of growth hormone enhances periimplant bone reaction in an osteoporotic rabbit model[J].Clin Oral Implants Res,2002,13(6):631-636.
[9]Tresguerres IF,Blanco L,Clemente C,et al.Effects of local administration of growth hormone in peri-implant bone:an experimental study with implants in rabbit tibiae[J].Internetional Journal of Oral Maxillofac Implants,2003,18(6):807-811.
[10]Tresguerres IF,Alobera MA,Baca R,et al.Histologic,morphometric,and densitometric study of peri-implant bone in rabbits with local administration of growth hormone[J].Internetional Journal of Oral Maxillofac Implants,2005,20(2):193-202.
[2]Baii H.J,Koibeck S,Krummrey G,et al.Systemic appiication of growth hormone for enhancement of secondary and intramembranous fracture heaiing[J].Horm Res,2002,58(3):39-42.
[3]Barake M,Klibanski A,Tritos NA.Effects of recombinant human growth hormone therapy on bone mineral density in adults with growth hormone deficiency:a meta-analysis[J].The Journal of clinical Endocrinology and Metabolism,2014,99(3):852-860.
[4]朱玉芳,潘革.GH-IGF-1轴与儿童生长发育[J].医学综述,2008,14(3):321-323.
[5]Zhao G,Monier-Faugere MC,et al.Targeted overexpression of insulin-like growth factor I to osteoblasts of transgenic mice:increased trabecular bone volume without increased osteoblast proliferation[J].Endocrinology,2000,141(7):2674-2682.
[6]Chia DJ.Minireview:Mechanisms of growth hormone-mediated gene regulation[J].Molecular Endorcrinology,2014,28(7):1012-1025.
[7]Calvo-Guirado JL,Mate-Sanchez J.Effects of growth hormone on initial bone formation around dental implants:a dog study[J].Clinical Oral Implants Research,2011,22(6):587-593.
[8]Tresguerres IF,Clemente C.Local administration of growth hormone enhances periimplant bone reaction in an osteoporotic rabbit model[J].Clin Oral Implants Res,2002,13(6):631-636.
[9]Tresguerres IF,Blanco L,Clemente C,et al.Effects of local administration of growth hormone in peri-implant bone:an experimental study with implants in rabbit tibiae[J].Internetional Journal of Oral Maxillofac Implants,2003,18(6):807-811.
[10]Tresguerres IF,Alobera MA,Baca R,et al.Histologic,morphometric,and densitometric study of peri-implant bone in rabbits with local administration of growth hormone[J].Internetional Journal of Oral Maxillofac Implants,2005,20(2):193-202.