具有铁氧化功能的硝基苯降解菌的筛选及特性
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摘要
采用硝基苯(NB)模拟废水对生物海绵铁体系进行驯化,筛选出一株具有铁氧化功能的高效硝基苯降解菌Y-9.经鉴定,菌株Y-9为节杆菌属(Arthrobacter sp.).通过平行对比实验,考察了菌株Y-9适宜的生长条件,研究了菌株Y-9介入下的海绵铁体系对NB的强化降解效果及降解机理.结果表明,菌株Y-9在硝基苯初始含量为200mg/L的条件下生长良好,且适宜的温度为25~40℃,适宜的p H值为6~8.此外,菌株Y-9作用下的海绵铁体系较单纯海绵铁体系及介入普通活性污泥的海绵铁体系对硝基苯的降解率分别提高88.6%和32.2%.生物海绵铁体系中Fe~(2+)、H_2O_2、?OH含量明显高于海绵铁体系,尤其是铁细菌Y-9作用下的生物海绵铁体系,Fe~(2+)、H_2O_2、?OH含量最高,为体系发生较强类Fenton效应提供了条件.通过对降解产物的定量分析发现,菌株Y-9降解硝基苯的途径遵循部分还原途径.本研究初步揭示了生物海绵铁体系类Fenton效应机理,为经济有效地处理硝基苯废水提供了新思路.
An efficient iron bacterium used to degrade nitrobenzene(NB)was isolated from the biological sponge iron system domesticated through NB simulated wastewater.The strain was identified as Arthrobacte sp.The suitable growing conditions,as well as the degradation effect and mechanism in sponge iron system with strain Y-9,were carried out by the contrast experiments.Results indicated that strain Y-9 grew well when the initial concentration of NB was 200mg/L,and the optimal conditions for the biodegradation of NB by strain Y-9 were at 25~40℃and p H 6~8.Besides,in contrast with the normal sponge irons system and the sponge irons system involved in ordinary activated sludge,the degradation rate of NB in biological sponge irons system at the present of strain Y-9 increased by 88.6%and 32.2%,respectively.The concentration of Fe~(2+),H_2O_2,and·OH in the biological sponge iron system,especially the system inoculated with strain Y-9,were significantly higher than that of normal sponge iron system,thus,it could promote Fenton-like effect.Quantitative analysis of the degradation products revealed that strain Y-9 followed the partial reductive degradation pathway of NB.The research revealed the mechanism of Fenton-like effect in a biological sponge iron system,and it also provided a new way to treat NB wastewater effectively.
An efficient iron bacterium used to degrade nitrobenzene(NB)was isolated from the biological sponge iron system domesticated through NB simulated wastewater.The strain was identified as Arthrobacte sp.The suitable growing conditions,as well as the degradation effect and mechanism in sponge iron system with strain Y-9,were carried out by the contrast experiments.Results indicated that strain Y-9 grew well when the initial concentration of NB was 200mg/L,and the optimal conditions for the biodegradation of NB by strain Y-9 were at 25~40℃and p H 6~8.Besides,in contrast with the normal sponge irons system and the sponge irons system involved in ordinary activated sludge,the degradation rate of NB in biological sponge irons system at the present of strain Y-9 increased by 88.6%and 32.2%,respectively.The concentration of Fe~(2+),H_2O_2,and·OH in the biological sponge iron system,especially the system inoculated with strain Y-9,were significantly higher than that of normal sponge iron system,thus,it could promote Fenton-like effect.Quantitative analysis of the degradation products revealed that strain Y-9 followed the partial reductive degradation pathway of NB.The research revealed the mechanism of Fenton-like effect in a biological sponge iron system,and it also provided a new way to treat NB wastewater effectively.
引文
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[21]林跃鑫,佘晨兴,许旭萍,等.氧化铁鞘细菌铁氧化酶最适产酶条件及其酶学特性的研究[J].水生生物学报,2004,28(4):385-390.
[22]姜成春,庞素艳,马军,等.钛盐光度法测定Fenton氧化中的过氧化氢[J].中国给水排水,2006,22(4):88-91.
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[26]Nishino S F,Spain J C.Degradation of Nitrobenzene by a Pseudomonas pseudoalcaligenes[J].Applied and Environmental Microbiology,1993,59(8):2520-2525.
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[28]Hofstetter T B,Spain J C,Nishino S F,et al.Identifying competing aerobic nitrobenzene biodegradation pathways by compound-specific isotope analysis[J].Environ.Sci.Technol.,2008,42(13):4764-4770.
[2]Rodriguez M,Timokhin V,Michl F,et al.The influence of different irradiation sources on the treatment of nitrobenzene[J].Catalysis Today,2002,76(2):291-300.
[3]Li Z L,Yang M,Li D,et al.Nitrobenzene biodegradation ability of microbial communities in water and sediments along the Songhua River after a nitrobenzene pollution event[J].Journal of Environmental Sciences,2008,20(7):778-786.
[4]Duan H T,Liu Y,Yin X H,et al.Degradation of nitrobenzene by Fenton-like reaction in a H2O2/schwertmannite system[J].Chemical Engineering Journal,2016,283:873-879.
[5]Carlos L,Nichela D,Triszcz J M,et al.Nitration of nitrobenzene in Fenton's processes[J].Chemosphere,2010,80(3):340-345.
[6]孙茜茜,李睿华,胡俊松,等.臭氧曝气沸石生物滤池处理硝基苯废水[J].环境工程学报,2016,10(6):3012-3016.
[7]余丽胜,焦纬洲,刘有智,等.超声强化铁碳微电解-Fenton法降解硝基苯废水[J].化工学报,2017,68(1):297-304.
[8]Jiao W Z,Feng Z R,Liu Y Z,et al.Degradation of nitrobenzenecontaining wastewater by carbon nanotubes immobilized nanoscale zerovalent iron[J].Journal of Nanoparticle Research,2016,18(7):1-9.
[9]Hu S,Wu Y,Yao H,et al.Enhanced Fenton-like removal of nitrobenzene via internal microelectrolysis in nano zerovalent iron/activated carbon composite[J].Water Science&Technology,2016,73(1):153-160.
[10]Ghiorse W C.Biology of iron and manganese depositing bacteria[J].Annual Review of Microbiology,1984,38(1):515-50.
[11]张艳,王宏武,马鲁铭.铁细菌在水处理方面的应用及影响其生长的因素[J].四川环境,2006,25(5):61-64.
[12]Voegelin A,Hug S J.Catalyzed oxidation of arsenic(III)by hydrogen peroxide on the surface of ferrihydrite:an in situ ATR-FTIR study[J].Environmental Science&Technology,2003,37(5):972-978.
[13]Wang X M,Waite T D.Iron speciation and iron species transformation in activated sludge membrane bioreactors[J].Water Research,2010,44(11):3511-3521.
[14]冯娟娟,王亚娥,李杰,等.新型富铁多孔复合填料强化生化处理效能研究[J].中国给水排水,2013,29(3):24-28.
[15]张迎迎,迟向群,王道胜,等.类产碱假单胞菌JS45的硝基苯降解动力学.环境工程学报[J].2017,11(2):1257-1261.
[16]Wang D,Zheng G,Zhou L.Isolation and characterization of a nitrobenzene-degrading bacterium Klebsiella ornithinolytica NB1from aerobic granular sludge[J].Bioresource Technology,2012,110(4):91-96.
[17]Tian L,Deng X,Wang J,et al.Biodegradation of nitrobenzene in a lysogeny broth medium by a novel halophilic bacterium Bacillus licheniformis[J].Marine Pollution Bulletin,2014,89(1/2):384-389.
[18]Zheng C L,Zhou J T,Wang J,et al.Isolation and characterization of a nitrobenzene degrading yeast strain from activated sludge[J].Journal of Hazardous Materials,2008,160(1):194-199.
[19]Liu N,Li H J,Ding F,et al.Analysis of biodegradation byproducts of nitrobenzene and aniline mixture by a cold-tolerant microbial consortium[J].Journal of Hazardous Materials,2013,260(18):323-329.
[20]马溪平,李鲜珠,李万龙,等.低温高盐条件下活性污泥降解水中硝基苯的研究[J].辽宁大学学报(自然科学版),2016,43(2):183-188.
[21]林跃鑫,佘晨兴,许旭萍,等.氧化铁鞘细菌铁氧化酶最适产酶条件及其酶学特性的研究[J].水生生物学报,2004,28(4):385-390.
[22]姜成春,庞素艳,马军,等.钛盐光度法测定Fenton氧化中的过氧化氢[J].中国给水排水,2006,22(4):88-91.
[23]Franzmann P D,Haddad C M,Hawkes R B,et al.Effects of temperature on the rates of iron and sulfur oxidation by selected bioleaching Bacteria and Archaea:Application of the Ratkowsky equation[J].Minerals Engineering,2005,18(13):1304-1314.
[24]Keenan C R,Sedlak D L.Factors affecting the yield of oxidants from the reaction of nanoparticulate zero-valent iron and oxygen[J].Environ.Sci.Technol.,2008,42(4):1262-1267.
[25]Joo S H,Feitz A J,Waite T D.Oxidative degradation of the carbothioate herbicide,molinate,using nanoscale zero-valent iron[J].Environ.Sci.Technol.,2004,38(7):2242-2247.
[26]Nishino S F,Spain J C.Degradation of Nitrobenzene by a Pseudomonas pseudoalcaligenes[J].Applied and Environmental Microbiology,1993,59(8):2520-2525.
[27]Nishino S F,Spain J C.Oxidative Pathway for the Biodegradation of Nitrobenzene by Comamonas SP.Strain JS765[J].Applied and Environmental Microbiology,1995,61(6):2308-2313.
[28]Hofstetter T B,Spain J C,Nishino S F,et al.Identifying competing aerobic nitrobenzene biodegradation pathways by compound-specific isotope analysis[J].Environ.Sci.Technol.,2008,42(13):4764-4770.