摘要
[目的]确定铁皮石斛花中总黄酮超声辅助提取最佳工艺条件,并对其抗氧化活性进行评价。[方法]采用正交试验方法,以总黄酮提取率为考察指标,对铁皮石斛花总黄酮提取方法的影响因素进行探讨,并对铁皮石斛花总黄酮提取工艺进行优化。以羟自由基清除率、DPPH和ABTS自由基清除率为指标,对铁皮石斛花总黄酮的体外抗氧化活性进行评价。[结果]铁皮石斛花总黄酮的最佳提取条件:乙醇体积分数80%,料液比1:50,提取温度55℃,提取次数2次,在以上条件下,总黄酮提取率达1.99%。铁皮石斛花总黄酮对羟自由基、DPPH和ABTS自由基有较强的清除作用,且羟自由基抗氧化活性与总黄酮含量呈正相关。[结论]利用正交法分析结果可靠,得到了铁皮石斛花总黄酮超声提取的最佳工艺条件,且实验结果还表明铁皮石斛花具有较强的抗氧化活性。
[Objective] To explore the optimal ultrasonic-assisted extraction conditions of total flavonoids from D-endrobium officinale flower and evaluate the antioxidant activity. [Methods] Orthogonal experiment was conducted to study the influence factor of extracting total flavones from Dendrobium officinale flower,the yield of total flavones as inspection index,and then optimized the extraction technology. The antioxidant effects of the total flavones were determined by hydroxyl, DPPH and ABTS radical scavenging capacities. [Results] The optimal extraction conditions of total flavonoids were ethanol concentration of 80%,material-liquid ratio of 1:50,extraction temperature of 55℃ and 2 extraction times. Under the optimal conditions,the maximum extraction rate of total flavonoids was 1.99%. Extracted total flavonoids had strong scavenging capacity on hydroxyl free radicals, DPPH and ABTS free radicals. [Conclusion] Orthogonal experiment is applicable for the optimization of ultrasonic extraction of total flavonoids from Dendrobium officinale flower.The total flavonoids from Dendrobium officinale flower have strong antioxidant capacity.
引文
[1]吕圭源,颜美秋,陈素红.铁皮石斛功效相关药理作用研究进展[J].中国中药杂志,2013,38(4):489-493.LV Guiyuan,YAN Meiqiu,CHEN Suhong.Review of pharmacological activities of Dendrobium officinale based on traditional functions[J].Chinese Journal of traditional Chinese Medicine,2013,38(4):489-493.
[2]霍昕,周建华,杨迺嘉,等.铁皮石斛花挥发性成分研究[J].中华中医药杂志,2008,23(8):735-737.HUO Xin,ZHOU Jianhua,YANG Naijia,et al.Determination of chemical constituents of essential oil fromflower of Dendrobium candidum Wall.ex Lind1[J].Chinese Journal of traditional Chinese Medicine,2008,23(8):735-737.
[3]何晓艳,吴人照,杨兵勋,等.铁皮石斛花对自发性高血压大鼠降压作用研究[J].浙江中医杂志,2016,51(2):152-154.HE Xiaoyan,WU Renzhao,YANG Bingxun,et al.Study on the antihypertensive effect of Dendrobium officinale flowers in spontaneously hypertensive rats[J].Zhejiang Journal of Traditional Chinese Medicine,2016,51(2):152-154.
[4]陈晓梅,王春兰,杨峻山,等.铁皮石斛化学成分及其分析的研究进展[J].中国药学杂志,2013,48(19):1634-1640.CHEN Xiaomei,WANG Chunlan,YANG Junshan,et al.Research Progress on Chemical Composition and Chemical Analysis of Dendrobium officinale[J].Chinese Journalof Pharmaceutical Sciences,2013,48(19):1634-1640.
[5]吕素华,徐萌,张新凤,等.11个铁皮石斛杂交家系鲜花的挥发性成分分析[J].中国实验方剂学杂志,2016,22(6):52-57.LV Suhua,XU Meng,ZHANG Xinfeng,et al.Studies on Volatile Constituents of 11 Families of Dendrobium officinale Flowers[J].Chinese Journal of Experimental Medicine,2016,22(6):52-57.
[6]李文静,李进进,李桂锋,等.GC-MS分析4种石斛花挥发性成分[J].中药材,2015,38(4):777-780.LI Wenjing,LI Jinjin,LI Guifeng,et al.Analysis of 4species of Dendrobium flower volatile components by GC-MS[J].Journal of Chinese Medicinal Material s,2015,38(4):777-780.
[7]周桂芬,吕圭源.铁皮石斛不同部位黄酮碳苷类成分及清除DPPH自由基能力比较研究[J].中国中药杂志,2012,37(11):1536-1540.ZHOU Guifen,LV Guiyuan.Comparative studies on scavenging DPPH free radicals activity of flavone C-glycosides from different parts of Dendrobium officinale[J].Chinese Journal of Traditional Chinese Medicine,2012,37(11):1536-1540.
[8]龚庆芳,何金祥,黄宁珍,等.铁皮石斛花化学成分及抗氧化活性研究[J].食品科技,2014,39(12):106-110.GONG Qingfang,HE Jinxiang,HUANG Ningzhen,et al.Chemical composition and antioxidant activities effec ts of Dendrobium offi cinale flower[J].Food Scienc e and Technology,2014,39(12):106-110.
[9]雷珊珊,吕圭源,金泽武,等.铁皮石斛花提取物对甲亢型阴虚小鼠的影响[J].中国中药杂志,2015,40(9):1793-1797.LEI Shanshan,LV Guiyuan,JIN Zewu,et al.Effect of extracts from Dendrobii ifficinalis flos on hyperthyroidism Yin deficiency mice[J].Chinese Journal of Traditional Chinese Medicine,2015,40(9):1793-1797.
[10]林为艺,颜美秋,吕圭源,等.铁皮石斛茎非多糖与粗多糖体内外抗氧化活性的比较[J].中药药理与临床,2016,32(2):138-141.LIN Weiyi,YAN Meiqiu,LV Guiyuan,et al.Antioxidant activity comparison between non-polysaccharides and crude polysaccharides from Dendrobium officinale stems in vitro and in vivo[J].Pharmacologyand clinic of Chinese medicine,2016,32(2):138-141.
[11]LUO AX,HE XJ,ZHOU S,et al.Purification,composition analysis and antioxidant activity of the polysaccharides from Dendrobium nobile Lindl[J].Car-bohydrate polymers,2010,79(4):1014-1019.
[12]周桂芬,吕圭源.基于高效液相色谱-二极管阵列光谱检测-电喷雾离子化质谱联用鉴定铁皮石斛叶中8种黄酮碳苷化合物及裂解规律研究[J].中国药学杂志,2012,47(1):13-19.ZHOU Guifen,LV Guiyuan.Study on Eight Flavone C-glycosides in Dendrobium officinale Leaves and their Fragmentation Pattern by HPLC-DAD-ESI-MSn[J].Chinese Journal of Pharmaceutical Sciences,2012,47(1):13-19.
[13]周桂芬,陈素红,吕圭源.浙江省铁皮石斛黄酮类成分高效液相色谱指纹图谱的建立[J].中国药学杂志,2013,48(15):1261-1265.ZHOU Guifen,CHEN Suhong,LV Guiyuan.HPLC Fingerprint of Flavonoids in Dendrobium officinale from Zhejiang Province[J].Chinese Journal of Pharmaceutical Sciences,2013,48(15):1261-1265.
[14]黄琴,沈杨霞,张成静,等.铁皮石斛多酚和黄酮含量及与抗氧化活性的相关性[J].应用与环境生物学报,2014,20(3):438-442.HUANG Qin,SHEN Yangxia,ZHANG Chengjing,et al.Correlation of the antioxidant property with the total phenolic content and total flavonoids of different Dendrobium offi cinale extracts[J].Chinese Journal of Applied Environmental Biology,2014,20(3):438-442.