女萎化学成分研究
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摘要
目的对毛茛科铁线莲属植物女萎Clematis apiifolia的化学成分进行研究。方法通过硅胶、ODS、凝胶Sephadex LH-20、HW-40C等多种色谱分离技术分离和纯化,利用核磁共振(NMR)及质谱等现代波谱学技术鉴定化合物结构。结果从女萎乙醇提取物中分离得到20个化合物,分别鉴定为常春藤皂苷元(1)、α-hederin(2)、sapindosideB(3)、豆甾-22-烯-3β,6β,9β-三醇(4)、pleuchiol(5)、豆甾醇-3-O-β-D-葡萄糖苷(6)、豆甾醇(7)、毒马草素(8)、莽草酸(9)、乙基-α-D-葡萄糖苷(10)、乙基-β-D-葡萄糖苷(11)、D-葡萄糖醛酸(12)、3,5-二羟基-4-戊内酯(13)、2-醛基-5-羟甲基呋喃(14)、尿嘧啶(15)、尿嘧啶核苷(16)、正二十三烷醇(17)、正三十烷醇(18)、正二十六烷酸(19)、棕榈酸-1-甘油酯(20)。结论所有化合物均为首次从女萎中分离鉴定,其中化合物3为首次从铁线莲属植物中分离得到,化合物4、5、9～12为首次从毛茛科植物中分离得到。
Objective To investigate the chemical constituents from the stem of the Clematis apiifolia. Methods A variety of chromatographic methods were applied in isolation and purification including silica gel, ODS, Sephadex LH-20 gel, and HW-40 C and so on. Their structures were identified by the nuclear magnetic resonance(NMR), mass spectrometry and so on. Results These compounds were isolated and determined as hederagenin(1), α-hederin(2), sapindoside B(3), stigmast-22-en-3β, 6β, 9β-triol(4), pleuchiol(5), stigmasterol-3-O-β-D-glucopyranoside(6), stimasterol(7), siderin(8), shikimic acid(9), ethyl-α-D-glucopyranoside(10), ethyl-β-D-glucopyranoside(11), D-gulonic acid(12), 3,5-dihydroxyl-4-valerolactone(13), 2-formyl-5-hydroxymethylfuran(14), uracil(15), uridine(16), tricosanol(17), triacontanol(18), hexacosanoic acid(19), and monostearin(20). Conclusion All compounds are obtained from C. apiifolia for the first time including firstly reported compound 3 from Clematis plants and compounds 4, 5, 9—12 from Ranunculaceae family.
Objective To investigate the chemical constituents from the stem of the Clematis apiifolia. Methods A variety of chromatographic methods were applied in isolation and purification including silica gel, ODS, Sephadex LH-20 gel, and HW-40 C and so on. Their structures were identified by the nuclear magnetic resonance(NMR), mass spectrometry and so on. Results These compounds were isolated and determined as hederagenin(1), α-hederin(2), sapindoside B(3), stigmast-22-en-3β, 6β, 9β-triol(4), pleuchiol(5), stigmasterol-3-O-β-D-glucopyranoside(6), stimasterol(7), siderin(8), shikimic acid(9), ethyl-α-D-glucopyranoside(10), ethyl-β-D-glucopyranoside(11), D-gulonic acid(12), 3,5-dihydroxyl-4-valerolactone(13), 2-formyl-5-hydroxymethylfuran(14), uracil(15), uridine(16), tricosanol(17), triacontanol(18), hexacosanoic acid(19), and monostearin(20). Conclusion All compounds are obtained from C. apiifolia for the first time including firstly reported compound 3 from Clematis plants and compounds 4, 5, 9—12 from Ranunculaceae family.
引文
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[25]张艳军,聂辉,周德雄,等.桂野桐化学成分的研究[J].中草药,2017,48(11):2172-2176.
[2]朱琳,周谧,楚楚.女萎质量标准研究[J].中国现代中药,2018,20(1):46-48.
[3]Hao D C,Gu X J,Xiao P G,et al.Chemical and biological research of Clematis medicinal resources[J].Chin Sci Bull,2013,58(10):1120-1129.
[4]Pan L L,Wang X L,Zhang Q Y,et al.Boehmenan,a lignan from the Chinese medicinal plant Clematis armandii,induces apoptosis in lung cancer cells through modulation of EGF-dependent pathways[J].Phytomedicine,2016,23(5):468-476.
[5]Rakesh C,Suresh K,Anupam S.The genus Clematis(Raunculaceae):Chemical and pharmacological perspectives[J].J Ethnopharmacol,2012,143(1):116-150.
[6]欧莹宝,魏明杰,李贺然,等.黄背栎化学成分的研究[J].中草药,2013,44(14):1872-1876.
[7]Ding Q,Yang L X,Yang H W,et al.Cytotoxic and antibacterial triterpenoids derivatives from Clematis ganpiniana[J].J Ethnopharmacol,2009,126(3):382-385.
[8]倪君君,吴兆华,高慧媛,等.瘤果黑种草子的化学成分[J].沈阳药科大学学报,2007,24(4):215-219.
[9]Amaral M D C,Faria A D,Magalhaes A F,et al.Steroids and triterpenes from Eleocharis acutangula and E.sellowiana(Cyperaceae)[J].Phytochem Anal,2004,15(2):125-129.
[10]陈江弢,杨崇仁.绿粉藁本的化学成分研究[J].中药材,2006,29(9):918-919.
[11]黄艳萍,宋家玲,吴继平,等.凉粉草化学成分分离鉴定[J].中国实验方剂学杂志,2018,24(6):77-81.
[12]倪付勇,温建辉,李明,等.金银花化学成分研究[J].中草药,2017,48(18):3689-3692.
[13]Liu Y B,Cheng X R,Qin J J,et al.Chemical constituents of Toona ciliata var.pubescens[J].Chin J Nat Med,2011,9(2):115-119.
[14]袁经权,周小雷,王硕,等.八角茴香化学成分的研究[J].中成药,2010,32(12):2123-2126.
[15]李云秋,王宾,杨斌,等.南海海绵Haliclona sp.的化学成分研究[J].热带海洋学报,2011,30(1):124-126.
[16]Wang X N,Du J C,Tan R X,et al.Chemical constituents of basidiomycete Hydnum repandum[J].中草药,2005,36(8):1126-1130.
[17]于泉林,高文远,张彦文,等.积雪草化学成分研究[J].中国中药杂志,2007,32(12):1182-1184.
[18]刘建宇,周楠,宫益霞,等.东北铁线莲化学成分的分离与鉴定[J].中国药物化学杂志,2016,26(1):56-60.
[19]牛雪梅,黎胜红,纳智,等.疏花毛萼香茶菜的化学成分研究(英文)[J].中草药,2003,34(4):300-303.
[20]张鹏耀,舒任庚,严喜鸾,等.山腊梅化学成分研究[J].中国药学杂志,2017,52(18):1594-1596.
[21]Peng X,Tan L,Yao B,et al.Studies on the constituents from the rhizoma of Alisma orientalis[J].J Chin Pharm Sci,1999,8(3):173-174.
[22]石磊岭,郭宝林,李广志,等.天山假狼毒地上部分化学成分研究[J].天然产物研究与开发,2017,29(1):74-78.
[23]罗超,陈重,张文芳,等.蛇足石杉化学成分研究[J].中草药,2011,42(12):2407-2409.
[24]翟城,乐世俊,林航,等.红花化学成分研究[J].中草药,2015,46(13):1872-1877.
[25]张艳军,聂辉,周德雄,等.桂野桐化学成分的研究[J].中草药,2017,48(11):2172-2176.