硒对铜胁迫酿酒葡萄幼苗生理特性的影响
|
摘要
【目的】探讨亚硒酸钠(Na_2SeO_3)对铜胁迫酿酒葡萄幼苗生理特性的影响,为揭示硒元素缓解酿酒葡萄幼苗铜胁迫作用提供参考依据。【方法】以宁夏贺兰山东麓酿酒葡萄产区淡灰钙土在温室中盆栽酿酒葡萄赤霞珠,分析添加外源硒(1.0 mg/kg)对铜胁迫(300.0 mg/kg)酿酒葡萄幼苗叶片光合色素含量、抗氧化酶[过氧化物酶(POD)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)]活性、丙二醛(MDA)和可溶性蛋白含量的影响。【结果】与对照(CK)相比,1.0 mg/kg硒处理能提高酿酒葡萄幼苗叶片的叶绿素a(Chl a)、叶绿素b(Chl b)和总叶绿素(Chl a+b)含量及POD和CAT活性,降低MDA含量,但对SOD活性和可溶性蛋白含量无显著影响(P>0.05);300.0 mg/kg铜胁迫可显著降低葡萄幼苗叶片的叶绿素(Chl)和可溶性蛋白含量(P<0.05,下同),提高Chl a/b、抗氧化酶活性及MDA含量;1.0 mg/kg硒+300.0 mg/kg铜处理的葡萄幼苗叶片Chl和可溶性蛋白含量与CK相当,但均高于300.0 mg/kg铜胁迫处理,SOD、POD和CAT活性比300.0 mg/kg铜胁迫处理略有降低,MAD含量显著低于300.0 mg/kg铜胁迫处理。【结论】铜胁迫条件下,低浓度(1.0 mg/kg)硒元素能提高酿酒葡萄幼苗对逆境的耐受力,提高叶片抗氧化酶活性,降低叶片细胞膜质过氧化程度,进而缓解铜对葡萄幼苗的毒害作用。
【Objective】Influences of sodium selenite(Na_2SeO_3)on physiological characteristics of wine grape seedlings under copper stress were studied to reveal that selenium(Se)element could alleviate the copper stress effects on wine grape seedling.【Method】A greenhouse pot experiment was conducted to explore the influences of exogenous Se(1.0 mg/kg)on leaf chlorophyll pigment content,antioxidant enzyme[peroxidase(POD),catalase(CAT) and superoxide dismutase(SOD)]activities,malondialdehyde(MDA)content and soluble protein content of wine grape seedling exposed to 300.0 mg/kg copper in the soil. The experimental variety of wine grape was Cabernet Sauvignon and the soil was light sierozem from east foothill of Helan Mountain,Ningxia.【Result】Compared with control(CK)treatment,1.0 mg/kg Se treatment could improve leaf chlorophyll a(Chl a)content,chlorophyll b(Chl b)content,total chlorophyll(Chl a+b)content,POD activity and CAT activity,decreased MAD content,but had insignificant difference(P>0.05). 300.0 mg/kg copper stress treatment decreased significantly(P<0.05,the same below)leaf chlorophyll content and soluble protein content of wine grape leaves,but increased Chl a/b content and antioxidant enzyme activities. In 1.0 mg/kg Se+300.0 mg/kg copper treatment,Chl content and soluble protein content in leaves were close to those in CK,but higher than those in 300.0 mg/kg copper stress treatment. In addition,compared with 300.0 mg/kg copper stress treatment,activities of SOD,POD and CAT decreased slightly,and MDA content was significantly lower.【Conclusion】Under copper stress,low concentration of Se(1.0 mg/kg)can improve the tolerance of wine grape seedlings to stress,improve the activity of leaf antioxidant enzymes and decrease the degree of cellulose peroxidation level,therefore it can alleviate the damage to grape seedlings by copper.
【Objective】Influences of sodium selenite(Na_2SeO_3)on physiological characteristics of wine grape seedlings under copper stress were studied to reveal that selenium(Se)element could alleviate the copper stress effects on wine grape seedling.【Method】A greenhouse pot experiment was conducted to explore the influences of exogenous Se(1.0 mg/kg)on leaf chlorophyll pigment content,antioxidant enzyme[peroxidase(POD),catalase(CAT) and superoxide dismutase(SOD)]activities,malondialdehyde(MDA)content and soluble protein content of wine grape seedling exposed to 300.0 mg/kg copper in the soil. The experimental variety of wine grape was Cabernet Sauvignon and the soil was light sierozem from east foothill of Helan Mountain,Ningxia.【Result】Compared with control(CK)treatment,1.0 mg/kg Se treatment could improve leaf chlorophyll a(Chl a)content,chlorophyll b(Chl b)content,total chlorophyll(Chl a+b)content,POD activity and CAT activity,decreased MAD content,but had insignificant difference(P>0.05). 300.0 mg/kg copper stress treatment decreased significantly(P<0.05,the same below)leaf chlorophyll content and soluble protein content of wine grape leaves,but increased Chl a/b content and antioxidant enzyme activities. In 1.0 mg/kg Se+300.0 mg/kg copper treatment,Chl content and soluble protein content in leaves were close to those in CK,but higher than those in 300.0 mg/kg copper stress treatment. In addition,compared with 300.0 mg/kg copper stress treatment,activities of SOD,POD and CAT decreased slightly,and MDA content was significantly lower.【Conclusion】Under copper stress,low concentration of Se(1.0 mg/kg)can improve the tolerance of wine grape seedlings to stress,improve the activity of leaf antioxidant enzymes and decrease the degree of cellulose peroxidation level,therefore it can alleviate the damage to grape seedlings by copper.
引文
卜元卿,石利利,单正军.2013.波尔多液在苹果和土壤中残留动态及环境风险评价[J].农业环境科学学报,32(5):972-978.[Bu Y Q,Shi L L,Shan Z J.2013.Residue dynamic and environmental risk assessment of bordeaux mixture in apple and soil[J].Journal of Agro-Environment Science,32(5):972-978.]
葛淑芳,章艺,梅笑漫,吴玉环,肖有铁,徐根娣,刘鹏.2014.外源水杨酸对铜胁迫下烟草叶片碳代谢的影响[J].水土保持学报,28(4):324-329.[Ge S F,Zhang Y,Mei XM,Wu Y H,Xiao Y T,Xu G D,Liu P.2014.Effect of exogenous salicylic acid on carbon metabolism of tobacco leaves under copper stress[J].Journal of Soil and Water Conservation,28(4):324-329.]
胡斌,王丹,王松山,周盟,梁东丽.2011.铜、硒复合污染对小白菜生长及金属吸收和转运的影响[J].环境科学学报,31(9):2033-2041.[Hu B,Wang D,Wang S S,Zhou M,Liang D L.2011.Uptake and transport of exogenous Cu and Se in Pak choi and their effects on growth[J].Acta Scientiae Circumstantiae,31(9):2033-2041.]
黄丽美,徐宁彤,曲琪环.2017.硒对玉米产量及籽粒营养品质、重金属含量的影响[J].江苏农业科学,45(10):59-61.[Huang L M,Xu N T,Qu Q H.2017.Effects of spraying selenium on yield,grain nutritional quality,and heavy metal contents of maize[J].Jiangsu Agricultural Sciences,45(10):59-61.]
李瑞平,李光德,袁宇飞,侯存东,于冲.2011.硒对汞胁迫小麦幼苗生理特性的影响[J].生态环境学报,20(5):975-979.[Li R P,Li G D,Yuan Y F,Hou C D,Yu C.2011.Effects of Se on some physiological characteristics of wheat seedling under Hg stress[J].Ecology and Environmental Sciences,20(5):975-979.]
李璇,岳红,王升,黄璐琦,马炯,郭兰萍.2013.影响植物抗氧化酶活性的因素及其研究热点和现状[J].中国中药杂志,38(7):973-978.[Li X,Yue H,Wang S,Huang L Q,Ma J,Guo L P.2013.Research of different effects on activity of plant antioxidant enzymes[J].China Journal of Chinese Material Medical,38(7):973-978.]
李永杰,李吉跃,方晓娟,张宽义.2010.铜胁迫对白蜡幼苗叶绿素含量及光合特性影响[J].东北林业大学学报,38(6):35-37.[Li Y J,Li J Y,Fang X J,Zhang K Y.2010.Effects of copper stress on chlorophyll contents and photosynthetic characteristics of Fraxinus chinensis seedlings[J].Journal of Northeast Forestry University,38(6):35-37.]
林义章,张淑媛,朱海生.2008.铜胁迫对小白菜叶肉细胞超微结构的影响[J].中国生态农业学报,16(4):948-951.[Lin Y Z,Zhang S Y,Zhu H S.2008.Effect of copper stress on ultra-structure of mesophyll cells in Chinese cabbage[J].Chinese Journal of Eco-Agriculture,16(4):948-951.]
刘春梅,罗盛国,刘元英.2015.硒对镉胁迫下寒地水稻镉含量与分配的影响[J].植物营养与肥料学报,21(1):190-199.[Liu C M,Luo S G,Liu Y Y.2015.Effects of Se on Cd content and distribution in rice plant under Cd stress in cold climate[J].Journal of Plant Nutrition and Fertilizer,21(1):190-199.]
刘群龙,王朵,吴国良,郝国伟,郝燕燕,孙胜.2011.硒对酥梨叶片衰老及抗氧化酶系统的影响[J].园艺学报,38(11):2059-2066.[Liu Q L,Wang D,Wu G L,Hao G W,Hao Y Y,Sun S.2011.Effects of selenium on leaf senescence and antioxidase system in pyrus bretschneider‘Dangshan Suli’[J].Acta Horticulturae Sinica,38(11):2059-2066.]
宁婵娟,丁宁,吴国良,冀爱青,刘晓华,杨燕君.2013.喷硒时期与浓度对红富士苹果果实品质及各部位全硒和有机态硒含量的影响[J].植物营养与肥料学报,19(5):1109-1117.[Ning C J,Ding N,Wu G L,Ji A Q,Liu X H,Yang Y J.2013.Effects of different selenium spraying scheme on the fruit quality,totalselenium and organic selenium contents in‘Red Fuji’apple trees[J].Journal of Plant Nutrition and Fertilizer,19(5):1109-1117.]
彭玲,贾芬,田小平,杨晶,屈婵娟,赵小虎.2015.硒对油菜根尖镉胁迫的缓解作用[J].环境科学学报,35(8):2597-2604.[Peng L,Jia F,Tian X P,Yang J,Qu C J,Zhao XH.2015.Alleviation of cadmium stress on root tip of rape seedlings by selenium[J].Acta Scientiae Circumstantiae,35(8):2597-2604.]
谭明明,张新英,付秋实,贺忠群,王怀松.2014.嫁接对铜胁迫下甜瓜幼苗生理特性的影响[J].应用生态学报,25(12):3563-3572.[Tan M M,Zhang X Y,Fu Q S,He ZQ,Wang H S.2014.Effects of grafting on physiological characteristics of melon(Cucumismelo)seedlings under copper stress[J].Chinese Journal of Applied Ecology,25(12):3563-3572.]
王斐,姜淑苓,欧春青,毋永龙,王海波,李连文,马力.2014.施用氨基酸硒肥对梨体内硒含量的影响[J].植物营养与肥料学报,20(6):1577-1582.[Wang F,Jiang S L,Ou C Q,Wu Y L,Wang H B,Li L W,Ma L.2014.Effects of amino acids selenium fertilizer on selenium concentration of pear[J].Journal of Plant Nutrition and Fertilizer,20(6):1577-1582.]
王江宏,刘艳,桑敏,李爱芬,张成武.2011.硒对紫球藻抗氧化酶活性及丙二醛含量的影响[J].热带海洋学报,30(3):94-98.[Wang J H,Liu Y,Sang M,Li A F,Zhang CW.2011.Effect of selenium on antioxidant enzyme activity and malondialdehyde content of Porphyridium sp.[J].Journal of Tropical Oceanography,30(3):94-98.]
王丽霞.2010.硒元素的植物生理作用及生理机制研究进展[J].安徽农业科学,38(1):31-32.[Wang L X.2010.Research progress on physiological functionand mechanism of selenium for plants[J].Journal of Anhui Agricultural Science,38(1):31-32.]
王巧碧,赵欠,周才琼.2016.重金属铜对酿酒酵母的氧化胁迫及其应用研究进展[J].酿酒科技,(1):98-102.[Wang Q B,Zhao Q,Zhou C Q.2016.Research pro-gress in S.cerevisiae response to Cu-induced oxidative stress and its application[J].Liquor-Making Science&Technology,(1):98-102.]
王娅玲,李维峰,马巾媛,朱春梅.2016.Cu胁迫对大叶种茶树幼苗生理特征的影响[J].云南农业大学学报(自然科学版),30(2):368-371.[Wang Y L,Li W F,Ma J Y,Zhu C M.2016.Effects of Cu on the physiological response of large-leaf tea seedling[J].Journal of Yunnan Agricultural University(Natural Science),30(2):368-371.]
肖志华,张义贤,张喜文,李萍.2012.外源铅、铜胁迫对不同基因型谷子幼苗生理生态特性的影响[J].生态学报,32(3):889-897.[Xiao Z H,Zhang Y X,Zhang X W,Li P.2012.Effects of exogenous Pb and Cu stress on ecophysiological characteristics on foxtail millet seedlings of different genotypes[J].Acta Ecologica Sinica,32(3):889-897.]
徐秋桐,张莉,章明奎.2014.长期喷施波尔多液对葡萄园土壤、树体和径流中铜积累的影响[J].水土保持学报,28(2):195-198.[Xu Q T,Zhang L,Zhang M K.2014.Accumulation of copper in soil,plant tissue,and runoff in vineyard as affected by long-term application of Bordeaux mixture[J].Journal of Soil and Water Conservation,28(2):195-198.]
杨昱,秦樊鑫.2014.铜胁迫对大豆幼苗抗氧化系统的影响[J].作物杂志,(1):81-84.[Yang Y,Qin F X.2014.Effects of copper stress on antioxidant system and growth in soybean seedling[J].Crops,(1):81-84.]
张志良,瞿伟菁,李小芳.2009.植物生理学实验指导[M].第4版.北京:高等教育出版社.[Zhang Z L,Qu W J,Li XF.2009.Plant Physiology Experimental Guidance[M].The 4thEdition.Beijing:Higher Education Press.]
赵秀峰,张强,程滨,霍晓兰.2017.硒对砷胁迫下小白菜生理特性及砷吸收的影响[J].环境科学学报,doi:10.13671/j.hjkxxb.2017.0104.[Zhao X F,Zhang Q,Cheng B,Huo XL.2017.Effects of selenium on the physiological characteristics,uptake of As by pakchoi under As stress[J].Acta Scientiae Circumstantiae,doi:10.13671/j.hjkxxb.2017.0104.]
朱丽琴,魏钦平,许雪峰,韩振海,王小伟,李天忠.2007.葡萄对硒的吸收、分布和积累特性的初步研究[J].园艺学报,34(2):325-328.[Zhu L Q,Wei Q P,Xu X F,Han ZH,Wang X W,Li T Z.2007.Selenium absorption,distribution and accumulation in grapevine[J].Acta Horticulturae Sinica,34(2):325-328.]
G?secka M,Mleczek M,Siwulski M,Niedzielski P.2016.Phenolic composition and antioxidant properties of Pleurotusostreatus and Pleurotuseryngii enriched with selenium and zinc[J].European Food Research and Technology,242(5):723-732.
Li L,Zhou W H,Dai H X,Cao F B,Zhang G P,Wu F B.2012.Selenium reduces cadmium uptake and mitigates cadmium toxicity in rice[J].Journal of Hazardous Materials,(235-236):343-351.
Lombardi L,Sebastiani L.2005.Copper toxicity in prunuscerasifera:Growth and antioxidant enzymes responses of in vitro grown plants[J].Plant Science,168(1):797-802.
Proiettia P,Nasinia L,Buonoa D D,D’amato R,Tedeschini E,Businelli D.2013.Selenium protects olive(Olea europaea L.)from drought stress[J].Scientia Horticulturae,164:165-171.
Sun X Y,Liu L L,Zhao Y,Ma T T,Zhao F,Huang W D,Zhan J C.2016.Effect of copper stress on growth characteristics and fermentation properties of Saccharomyces cerevisiae and the pathway of copper adsorption during wine fermentation[J].Food Chemistry,192:43-52.
葛淑芳,章艺,梅笑漫,吴玉环,肖有铁,徐根娣,刘鹏.2014.外源水杨酸对铜胁迫下烟草叶片碳代谢的影响[J].水土保持学报,28(4):324-329.[Ge S F,Zhang Y,Mei XM,Wu Y H,Xiao Y T,Xu G D,Liu P.2014.Effect of exogenous salicylic acid on carbon metabolism of tobacco leaves under copper stress[J].Journal of Soil and Water Conservation,28(4):324-329.]
胡斌,王丹,王松山,周盟,梁东丽.2011.铜、硒复合污染对小白菜生长及金属吸收和转运的影响[J].环境科学学报,31(9):2033-2041.[Hu B,Wang D,Wang S S,Zhou M,Liang D L.2011.Uptake and transport of exogenous Cu and Se in Pak choi and their effects on growth[J].Acta Scientiae Circumstantiae,31(9):2033-2041.]
黄丽美,徐宁彤,曲琪环.2017.硒对玉米产量及籽粒营养品质、重金属含量的影响[J].江苏农业科学,45(10):59-61.[Huang L M,Xu N T,Qu Q H.2017.Effects of spraying selenium on yield,grain nutritional quality,and heavy metal contents of maize[J].Jiangsu Agricultural Sciences,45(10):59-61.]
李瑞平,李光德,袁宇飞,侯存东,于冲.2011.硒对汞胁迫小麦幼苗生理特性的影响[J].生态环境学报,20(5):975-979.[Li R P,Li G D,Yuan Y F,Hou C D,Yu C.2011.Effects of Se on some physiological characteristics of wheat seedling under Hg stress[J].Ecology and Environmental Sciences,20(5):975-979.]
李璇,岳红,王升,黄璐琦,马炯,郭兰萍.2013.影响植物抗氧化酶活性的因素及其研究热点和现状[J].中国中药杂志,38(7):973-978.[Li X,Yue H,Wang S,Huang L Q,Ma J,Guo L P.2013.Research of different effects on activity of plant antioxidant enzymes[J].China Journal of Chinese Material Medical,38(7):973-978.]
李永杰,李吉跃,方晓娟,张宽义.2010.铜胁迫对白蜡幼苗叶绿素含量及光合特性影响[J].东北林业大学学报,38(6):35-37.[Li Y J,Li J Y,Fang X J,Zhang K Y.2010.Effects of copper stress on chlorophyll contents and photosynthetic characteristics of Fraxinus chinensis seedlings[J].Journal of Northeast Forestry University,38(6):35-37.]
林义章,张淑媛,朱海生.2008.铜胁迫对小白菜叶肉细胞超微结构的影响[J].中国生态农业学报,16(4):948-951.[Lin Y Z,Zhang S Y,Zhu H S.2008.Effect of copper stress on ultra-structure of mesophyll cells in Chinese cabbage[J].Chinese Journal of Eco-Agriculture,16(4):948-951.]
刘春梅,罗盛国,刘元英.2015.硒对镉胁迫下寒地水稻镉含量与分配的影响[J].植物营养与肥料学报,21(1):190-199.[Liu C M,Luo S G,Liu Y Y.2015.Effects of Se on Cd content and distribution in rice plant under Cd stress in cold climate[J].Journal of Plant Nutrition and Fertilizer,21(1):190-199.]
刘群龙,王朵,吴国良,郝国伟,郝燕燕,孙胜.2011.硒对酥梨叶片衰老及抗氧化酶系统的影响[J].园艺学报,38(11):2059-2066.[Liu Q L,Wang D,Wu G L,Hao G W,Hao Y Y,Sun S.2011.Effects of selenium on leaf senescence and antioxidase system in pyrus bretschneider‘Dangshan Suli’[J].Acta Horticulturae Sinica,38(11):2059-2066.]
宁婵娟,丁宁,吴国良,冀爱青,刘晓华,杨燕君.2013.喷硒时期与浓度对红富士苹果果实品质及各部位全硒和有机态硒含量的影响[J].植物营养与肥料学报,19(5):1109-1117.[Ning C J,Ding N,Wu G L,Ji A Q,Liu X H,Yang Y J.2013.Effects of different selenium spraying scheme on the fruit quality,totalselenium and organic selenium contents in‘Red Fuji’apple trees[J].Journal of Plant Nutrition and Fertilizer,19(5):1109-1117.]
彭玲,贾芬,田小平,杨晶,屈婵娟,赵小虎.2015.硒对油菜根尖镉胁迫的缓解作用[J].环境科学学报,35(8):2597-2604.[Peng L,Jia F,Tian X P,Yang J,Qu C J,Zhao XH.2015.Alleviation of cadmium stress on root tip of rape seedlings by selenium[J].Acta Scientiae Circumstantiae,35(8):2597-2604.]
谭明明,张新英,付秋实,贺忠群,王怀松.2014.嫁接对铜胁迫下甜瓜幼苗生理特性的影响[J].应用生态学报,25(12):3563-3572.[Tan M M,Zhang X Y,Fu Q S,He ZQ,Wang H S.2014.Effects of grafting on physiological characteristics of melon(Cucumismelo)seedlings under copper stress[J].Chinese Journal of Applied Ecology,25(12):3563-3572.]
王斐,姜淑苓,欧春青,毋永龙,王海波,李连文,马力.2014.施用氨基酸硒肥对梨体内硒含量的影响[J].植物营养与肥料学报,20(6):1577-1582.[Wang F,Jiang S L,Ou C Q,Wu Y L,Wang H B,Li L W,Ma L.2014.Effects of amino acids selenium fertilizer on selenium concentration of pear[J].Journal of Plant Nutrition and Fertilizer,20(6):1577-1582.]
王江宏,刘艳,桑敏,李爱芬,张成武.2011.硒对紫球藻抗氧化酶活性及丙二醛含量的影响[J].热带海洋学报,30(3):94-98.[Wang J H,Liu Y,Sang M,Li A F,Zhang CW.2011.Effect of selenium on antioxidant enzyme activity and malondialdehyde content of Porphyridium sp.[J].Journal of Tropical Oceanography,30(3):94-98.]
王丽霞.2010.硒元素的植物生理作用及生理机制研究进展[J].安徽农业科学,38(1):31-32.[Wang L X.2010.Research progress on physiological functionand mechanism of selenium for plants[J].Journal of Anhui Agricultural Science,38(1):31-32.]
王巧碧,赵欠,周才琼.2016.重金属铜对酿酒酵母的氧化胁迫及其应用研究进展[J].酿酒科技,(1):98-102.[Wang Q B,Zhao Q,Zhou C Q.2016.Research pro-gress in S.cerevisiae response to Cu-induced oxidative stress and its application[J].Liquor-Making Science&Technology,(1):98-102.]
王娅玲,李维峰,马巾媛,朱春梅.2016.Cu胁迫对大叶种茶树幼苗生理特征的影响[J].云南农业大学学报(自然科学版),30(2):368-371.[Wang Y L,Li W F,Ma J Y,Zhu C M.2016.Effects of Cu on the physiological response of large-leaf tea seedling[J].Journal of Yunnan Agricultural University(Natural Science),30(2):368-371.]
肖志华,张义贤,张喜文,李萍.2012.外源铅、铜胁迫对不同基因型谷子幼苗生理生态特性的影响[J].生态学报,32(3):889-897.[Xiao Z H,Zhang Y X,Zhang X W,Li P.2012.Effects of exogenous Pb and Cu stress on ecophysiological characteristics on foxtail millet seedlings of different genotypes[J].Acta Ecologica Sinica,32(3):889-897.]
徐秋桐,张莉,章明奎.2014.长期喷施波尔多液对葡萄园土壤、树体和径流中铜积累的影响[J].水土保持学报,28(2):195-198.[Xu Q T,Zhang L,Zhang M K.2014.Accumulation of copper in soil,plant tissue,and runoff in vineyard as affected by long-term application of Bordeaux mixture[J].Journal of Soil and Water Conservation,28(2):195-198.]
杨昱,秦樊鑫.2014.铜胁迫对大豆幼苗抗氧化系统的影响[J].作物杂志,(1):81-84.[Yang Y,Qin F X.2014.Effects of copper stress on antioxidant system and growth in soybean seedling[J].Crops,(1):81-84.]
张志良,瞿伟菁,李小芳.2009.植物生理学实验指导[M].第4版.北京:高等教育出版社.[Zhang Z L,Qu W J,Li XF.2009.Plant Physiology Experimental Guidance[M].The 4thEdition.Beijing:Higher Education Press.]
赵秀峰,张强,程滨,霍晓兰.2017.硒对砷胁迫下小白菜生理特性及砷吸收的影响[J].环境科学学报,doi:10.13671/j.hjkxxb.2017.0104.[Zhao X F,Zhang Q,Cheng B,Huo XL.2017.Effects of selenium on the physiological characteristics,uptake of As by pakchoi under As stress[J].Acta Scientiae Circumstantiae,doi:10.13671/j.hjkxxb.2017.0104.]
朱丽琴,魏钦平,许雪峰,韩振海,王小伟,李天忠.2007.葡萄对硒的吸收、分布和积累特性的初步研究[J].园艺学报,34(2):325-328.[Zhu L Q,Wei Q P,Xu X F,Han ZH,Wang X W,Li T Z.2007.Selenium absorption,distribution and accumulation in grapevine[J].Acta Horticulturae Sinica,34(2):325-328.]
G?secka M,Mleczek M,Siwulski M,Niedzielski P.2016.Phenolic composition and antioxidant properties of Pleurotusostreatus and Pleurotuseryngii enriched with selenium and zinc[J].European Food Research and Technology,242(5):723-732.
Li L,Zhou W H,Dai H X,Cao F B,Zhang G P,Wu F B.2012.Selenium reduces cadmium uptake and mitigates cadmium toxicity in rice[J].Journal of Hazardous Materials,(235-236):343-351.
Lombardi L,Sebastiani L.2005.Copper toxicity in prunuscerasifera:Growth and antioxidant enzymes responses of in vitro grown plants[J].Plant Science,168(1):797-802.
Proiettia P,Nasinia L,Buonoa D D,D’amato R,Tedeschini E,Businelli D.2013.Selenium protects olive(Olea europaea L.)from drought stress[J].Scientia Horticulturae,164:165-171.
Sun X Y,Liu L L,Zhao Y,Ma T T,Zhao F,Huang W D,Zhan J C.2016.Effect of copper stress on growth characteristics and fermentation properties of Saccharomyces cerevisiae and the pathway of copper adsorption during wine fermentation[J].Food Chemistry,192:43-52.