铝灰渣提取物对大鼠亚急性毒性作用的研究
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摘要
为探索铝灰渣提取物对大鼠的亚急性毒性作用,将40只SPF级Wistar大鼠按性别、体重随机分为4组,分别为大、中、小剂量铝灰渣提取物(AAE)染毒组(依据体重,染毒剂量分别为15.60、7.80、5.20mg/kg)和对照组(蒸馏水),每组10只,连续灌胃染毒28 d。观察染毒前、后大鼠外观体征,取血检测外周血液学指标和血液生化指标,计算心、肝、脾、肺、肾、睾丸及副睾、子宫及卵巢等脏器系数,并对重要脏器进行组织形态学检查。结果,与对照组比较,各组大鼠自主活动明显减少,体重增长明显减慢;血液学指标、血液生化指标,脏器指数均出现明显差异;AAE大、中剂量组大鼠心、肝、脾、肺、肾、胃、结肠等脏器组织形态均有不同程度的病理改变。上述结果表明,铝灰渣提取物对大鼠心、肝、脾、肺、肾、胃、结肠等组织均有不同程度的病理损伤,损伤程度呈剂量依赖性。
To study the subacute toxicity of the aluminum ash extract in rat,forty Wistar rats were randomly divided into four groups according to gender and weight,large-dose,medium-dose and small-dose of aluminum ash extract(AAE) exposured group and control group(n=10).All of the AAE exposured groups were orally administrated with AAE at 15.60,7.80,and 5.20 mg/kg according to body weight,respectively,the control group was orally administrated with distilled water,lasting for 28 days.The general states was observed,the levels of hematology and serum biochemistry were tested,the organ coefficients of heart,liver,spleen,lung, kidney,testis and epididymis, uterus and ovary were calculated,and the important organs of rats was stained with hematoxylin-eosin(HE) staining to observe the changes of peritoneal morphology.In result,compared with the control group,the spontaneous activity in all of the AAE groups were significantly reduced,the weight gain was significantly slowed down,the hematology index and serum biochemistry index and organ index were appeared distinctly abnormal.The tissue morphology of heart,liver,spleen,lung,kidney,stomach and colon in the AAE large-dose or medium-dose group were presented morphologic changes.In conclusion,the AAE has pathological injury to heart,liver,spleen,lung,kidney,stomach and colon of rat,and the degree of damage depends on dose.
To study the subacute toxicity of the aluminum ash extract in rat,forty Wistar rats were randomly divided into four groups according to gender and weight,large-dose,medium-dose and small-dose of aluminum ash extract(AAE) exposured group and control group(n=10).All of the AAE exposured groups were orally administrated with AAE at 15.60,7.80,and 5.20 mg/kg according to body weight,respectively,the control group was orally administrated with distilled water,lasting for 28 days.The general states was observed,the levels of hematology and serum biochemistry were tested,the organ coefficients of heart,liver,spleen,lung, kidney,testis and epididymis, uterus and ovary were calculated,and the important organs of rats was stained with hematoxylin-eosin(HE) staining to observe the changes of peritoneal morphology.In result,compared with the control group,the spontaneous activity in all of the AAE groups were significantly reduced,the weight gain was significantly slowed down,the hematology index and serum biochemistry index and organ index were appeared distinctly abnormal.The tissue morphology of heart,liver,spleen,lung,kidney,stomach and colon in the AAE large-dose or medium-dose group were presented morphologic changes.In conclusion,the AAE has pathological injury to heart,liver,spleen,lung,kidney,stomach and colon of rat,and the degree of damage depends on dose.
引文
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[12]陈洁,赵世文,杨金龙.等.亚慢性铅与福单独或联合暴露致大鼠氧化损伤作用及α-硫辛酸的保护效应[J].中国兽医科学,2018,48(06):790-796.CHEN Jie,ZHAO Shi-wen,YANG Jin-long,et al.Oxidative damage of sub-chronic exposure to lead and/or cadmium in rats and the protection ofα-lipoic acid[J]. Chinese Veterinary Science,2018,48(06):790-796.(in Chinese)
[13]董朕,白玉彬,张继瑜,等.五氯柳胺毒性的研究进展[J].中国兽医科学,2018,48(07):905-909.DONG Zhen,BAI Yu-bin,ZHANG Ji-yu,et al.Progress in toxicity of oxyclozanide[J].Chinese Veterinary Science,2018,48(07):905-909.(in Chinese)
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[15]霍桂桃,吕建军,屈哲,等.毒性病理学评价中动物剖检及大体病理学检查原则[J].药物评价研究,2017,40(10):1365-1371.HUO Gui-tao,LüJian-jun,QU Zhe,et al.Principles of necropsy and gross pathology examination for toxicologic pathology assessment[J].Drug Evaluation Research,2017,40(10):1365-1371.(in Chinese)
[2]马英,杜建伟,郑娟,等.再生铝工业固体废物成分组成与污染特性研究[J].轻金属,2017(10):58-62.MA Ying,DU Jian-wei,ZHEN Juan,et al.Study on composition and pollution characteristics of solid waste in recycled aluminum industry[J].Light Metals,2017,10:58-62.(in Chinese)
[3]姜东生,李梅,崔益斌,等.重金属和氯酚对霍甫水丝蚓的急性毒性及水环境安全评价[J].中国环境科学,2014,34(6):1572-1578.JIANG Dong-sheng,LI Mei,CUI Yi-bin,et al.Acute toxic effects of heavy metals and chlorophenols on Limnodrilus hoffmeisteri and safety assessment of the aquatic environment[J].China Environmental Science,2014,34(6):1572-1578.(in Chinese)
[4]朱爱萍,陈建耀,高磊,等.北江上游水环境重金属污染及生态毒性的时空变化[J].环境科学学报,2015,35(8):2487-2496.ZHU Ai-ping,CHEN Jian-yao,GAO Lei,et al.Spatial and temporal variation of heavy metals and their association with ecotoxicity in the upper reaches of Wengjiang River in Beijiang Basin[J].Acta Scientiae Circumstantiae,2015,35(8):2487-2496.(in Chinese)
[5]崔慧慧,白晓琴,李莉,等.三氯化铝暴露致雄性小鼠生殖细胞的遗传毒性[J].环境与健康杂志,2009,26(1):68-70.CUI Hui-hui,BAI Xiao-qing,LI Li,et al.Genetic toxicity of aluminium trichloride in male mice[J].Journal of Environment and Health,2009,26(1):68-70.(in Chinese)
[6]段华波,黄启飞,王琪,等.危险废物浸出毒性的理论基础研究[J].环境科学研究,2005(S1):27-30.DUAN Hua-bo,HUANG Qi-fei,WANG Qi,et al.Study on theoretical basis of hazardous waste extraction toxicity[J].Research of Environmental Sciences,2005(S1):27-30.(in Chinese)
[7]何绪文,李静,石靖靖,等.镍冶炼废渣毒性浸出试验研究[J].环境工程,2014,32(S1):804-807.HE Xu-wen,LI Jing,SHI Jing-jing,et al.Test study on toxicity leaching of nickel smelting waste slag[J].EnvironmentalEngineering,2014,32(S1):804-807.(in Chinese)
[8]赵国华,罗兴章,陈贵,等.固体废物中重金属浸出毒性评价方法的研究进展[J].环境污染与防治,2013,35(7):80-84.ZHAO Guo-hua,LUO Xing-zhang,CHEN Gui,et al.Progress on the testing method of heavy metals leaching toxicity of solid waste[J].Environmental Pollution and Control,2013,35(7):80-84.(in Chinese)
[9]纪辛,郭鸿.固体废物焚烧灰渣毒性评估及其控制方法研究进展[J].化工科技,2008,16(5):62-67.JI Xing,GUO Hong.Research progress on toxicity assessment and control methods of solid waste incineration ash[J].Science and Technology in Chemical Industry,2008,16(5):62-67.(in Chinese)
[10]吴芳,罗爱平,李楠.含重金属水处理污泥的固化和浸出毒性研究[J].环境污染治理技术与设备,2003,4(12):40-43.WU Fang,LUO Ai-ping,LI Nan.Experimental study on solidification and soaking test of sewage sludge containing heavy metal ion[J].Techniques and Equipment for Environmental Pollution Control,2003,4(12):40-43.(in Chinese)
[11]路浩,张浩,赵宝玉,等.氯化汞对SD大鼠脑组织氧化损伤的影响[J].中国兽医学报,2012,32(1):122-124.LU Hao,ZHANG Hao,ZHAO Bao-yu,et al.Effect of mercuic chloride on oxidative damage of braintissues in SD rats[J].Chinese Journal of Veterinary Science,2012,32(1):122-124.(in Chinese)
[12]陈洁,赵世文,杨金龙.等.亚慢性铅与福单独或联合暴露致大鼠氧化损伤作用及α-硫辛酸的保护效应[J].中国兽医科学,2018,48(06):790-796.CHEN Jie,ZHAO Shi-wen,YANG Jin-long,et al.Oxidative damage of sub-chronic exposure to lead and/or cadmium in rats and the protection ofα-lipoic acid[J]. Chinese Veterinary Science,2018,48(06):790-796.(in Chinese)
[13]董朕,白玉彬,张继瑜,等.五氯柳胺毒性的研究进展[J].中国兽医科学,2018,48(07):905-909.DONG Zhen,BAI Yu-bin,ZHANG Ji-yu,et al.Progress in toxicity of oxyclozanide[J].Chinese Veterinary Science,2018,48(07):905-909.(in Chinese)
[14]牟文波,李慧,安会苹,等.幼年SD大鼠生化指标背景数据的建立和结果分析[J].中国药物警戒,2017,14(11):641-645.MU Wen-bo,LI Hui,AN Hui-ping,et al.Acquis ition and analys is of basic data of juvenile sprague dawley rats[J].Chinese Journal of Pharmacovigilance,2017,14(11):641-645.(in Chinese)
[15]霍桂桃,吕建军,屈哲,等.毒性病理学评价中动物剖检及大体病理学检查原则[J].药物评价研究,2017,40(10):1365-1371.HUO Gui-tao,LüJian-jun,QU Zhe,et al.Principles of necropsy and gross pathology examination for toxicologic pathology assessment[J].Drug Evaluation Research,2017,40(10):1365-1371.(in Chinese)