全清液发酵营养组学的精准控制
|
摘要
以黑曲霉Co827菌株为试验菌株,在淀粉全清液培养基的基础上,以多尺度手段对培养基中的氮源、磷源对菌株产酸能力的影响进行研究。研究结果表明:黑曲霉Co827以淀粉糖液为碳源的全清液发酵培养基中,生长期(种子罐培养基)的碳氮比为30、氮磷比为3.2,产酸期(发酵罐培养基)的碳氮比为70,氮磷比为3.5。
Aspergillus Niger Co827 was used as the experimental strain. On the basis of starch clear medium, the effect of nitrogen and phosphorus sources on the acidogenicity of this strain was studied by multi-scale method. The results showed that Aspergillus Niger Co827 was fermented in pure fermentation medium with starch sugar solution as carbon source. During the growth period(seed tank medium), the ratio of carbon to nitrogen was 30, the ratio of nitrogen to phosphorus was 3.2; the ratio of carbon to nitrogen in the acid-producing period(fermentation tank medium) was 70,and the ratio of nitrogen to phosphorus was 3.5.
Aspergillus Niger Co827 was used as the experimental strain. On the basis of starch clear medium, the effect of nitrogen and phosphorus sources on the acidogenicity of this strain was studied by multi-scale method. The results showed that Aspergillus Niger Co827 was fermented in pure fermentation medium with starch sugar solution as carbon source. During the growth period(seed tank medium), the ratio of carbon to nitrogen was 30, the ratio of nitrogen to phosphorus was 3.2; the ratio of carbon to nitrogen in the acid-producing period(fermentation tank medium) was 70,and the ratio of nitrogen to phosphorus was 3.5.
引文
[1]王博彦,金其荣.发酵有机酸生产与应用手册.北京:中国轻工业出版社,2000-01:124-149.
[2]夏胜洁.不同氮源对柠檬酸发酵的影响[R].石河子长运生化有限责任公司832000.
[3]孙维国.部分有机氮源对柠檬酸发酵的影响[R].宁夏宁馨儿生物工程股份有限公司.
[4]张伟心,王建平,等.有机氮源对柠檬酸发酵的影响[J].河北大学学报:自然科学版,2000(2).
[5]代真真,周勇,刘玉伟,满云,张继学,王永红,等,柠檬酸发酵过程中液化条件的影响[D].华东理工大学生物工程学院,上海.
[6]张嗣良,储炬.多尺度微生物过程优化[M].北京:化学工业出版社,2003:230-289
[2]夏胜洁.不同氮源对柠檬酸发酵的影响[R].石河子长运生化有限责任公司832000.
[3]孙维国.部分有机氮源对柠檬酸发酵的影响[R].宁夏宁馨儿生物工程股份有限公司.
[4]张伟心,王建平,等.有机氮源对柠檬酸发酵的影响[J].河北大学学报:自然科学版,2000(2).
[5]代真真,周勇,刘玉伟,满云,张继学,王永红,等,柠檬酸发酵过程中液化条件的影响[D].华东理工大学生物工程学院,上海.
[6]张嗣良,储炬.多尺度微生物过程优化[M].北京:化学工业出版社,2003:230-289