脂联素水平及位点的基因多态性与广东地区2型糖尿病的相关性
|
摘要
目的分析脂联素水平变化及脂联素rs1063539和rs3821799基因多态性与广东地区2型糖尿病的相关性。方法收集广医二院新发单纯2型糖尿病(T2DM)患者180例为病例组,健康人群192例为对照组,比较两组血清脂联素水平及rs1063539位点(C/G)、rs3821799位点(C/T)基因型分布频率,作Logistic回归分析。结果 T2DM组血清脂联素水平明显低于对照组,差异有统计学意义(P=0.001),T2DM组的血清甘油三酯明显高于对照组,高密度脂蛋白明显低于对照组,差异皆有统计学意义(P=0.01,P=0.03)。rs1063539基因型的分布频率差异具有统计学意义(P<0.05),rs3821799基因型的分布频率差异无统计学意义(P>0.05)。T2DM组rs1063539各基因型对应的TG差异皆有统计学意义(P=0.04,P=0.01),T2DM组rs3821799各基因型对应的生化指标差异无统计学意义(P>0.05)。结论脂联素可能与T2DM的发生发展密切相关;可作为评价T2DM的一种新指标。rs1063539病例组与对照组的基因型差异有统计学意义,提示rs1063539多态性可能与T2DM相关,GC基因型者具有较高的T2DM易感性。脂联素rs3821799基因型可能与广东地区T2DM的发生不相关。
Objective To explore the relationship among the changes of adiponectin levels,adiponectin SNPs rs1063539,rs3821799 and type2 diabetes mellitus(T2 DM)in Guangdong. Methods Totally 180 cases of newly diagnosed T2 DM patients were enrolled as T2 DM group and 192 healthy subjects as control group. The distribution frequency of adiponectin rs1063539 locus A/T and rs3821799 locus A/G between the two groups was compared,and then Logistic regression analysis was conducted. Results The adiponectin level of T2 DM group was significantly higher than that of control group(P = 0.001). Serum TG was significantly higher in the T2 DM group than that in the control group and HDL-C were significantly lower than that in the control group(P = 0.01 and0.03,respectively)and the differences were statistically significant. The difference of the frequency distribution of rs10635391 genotype between the two groups had statistical significance(P < 0.05)but the difference of the frequency distribution of rs3821799 genotype had no statistical significance(P > 0.05). The rs1063539 genotype of T2 DM group corresponding biochemical indicators indexed triglycerides(TG)had statistical difference(P = 0.04,0.01,respectively). The rs3821799 genotype of T2 DM group corresponding biochemical indicators had no statistical difference(P > 0.05). Conclusions Adiponectin could be used as a new index to evaluate insulin resistance and type 2 diabetes. rs1063539 genotype has correlation with T2 DM in Guangdong and GC may have a higher susceptibility to type 2 diabetes. Adiponectin rs3821799 genotype has no correlation with T2 DM in Guangdong.
Objective To explore the relationship among the changes of adiponectin levels,adiponectin SNPs rs1063539,rs3821799 and type2 diabetes mellitus(T2 DM)in Guangdong. Methods Totally 180 cases of newly diagnosed T2 DM patients were enrolled as T2 DM group and 192 healthy subjects as control group. The distribution frequency of adiponectin rs1063539 locus A/T and rs3821799 locus A/G between the two groups was compared,and then Logistic regression analysis was conducted. Results The adiponectin level of T2 DM group was significantly higher than that of control group(P = 0.001). Serum TG was significantly higher in the T2 DM group than that in the control group and HDL-C were significantly lower than that in the control group(P = 0.01 and0.03,respectively)and the differences were statistically significant. The difference of the frequency distribution of rs10635391 genotype between the two groups had statistical significance(P < 0.05)but the difference of the frequency distribution of rs3821799 genotype had no statistical significance(P > 0.05). The rs1063539 genotype of T2 DM group corresponding biochemical indicators indexed triglycerides(TG)had statistical difference(P = 0.04,0.01,respectively). The rs3821799 genotype of T2 DM group corresponding biochemical indicators had no statistical difference(P > 0.05). Conclusions Adiponectin could be used as a new index to evaluate insulin resistance and type 2 diabetes. rs1063539 genotype has correlation with T2 DM in Guangdong and GC may have a higher susceptibility to type 2 diabetes. Adiponectin rs3821799 genotype has no correlation with T2 DM in Guangdong.
引文
[1] DEABREU V,CJM M,PAC D,et al. High-molecular weight adiponectin/HOMA-IR ratio as a biomarker of metabolic syndrome in urban multiethnic Brazilian subjects[J]. PLOS ONE,2017:12(7):e0180947.
[2] KARINA G,HUAIEN Z,ANOUAR H,et al. Decreased adiponectin-mediated signaling through the adipoR2 pathway is associated with carotid plaque instability[J]. STROKE,2017:1-22.
[3] LIU Y,SWEENEY G. Adiponectin action in skeletal muscle[J].Best Pract Res Clin Endocrinol Matal,2014,28(1):33-41.
[4] LINDBERG S,JENSEN J S,BJERRE M,et al. Adiponectin,type 2 diabetes and cardiovascular risk[J]. Eur J Prev Cardiol,2015:22(3):276-83.
[5] MENZAGHI C,TRISCHITTA V. The adiponectin paradox for allcause and cardiovascular mortality[J]. Diabetes,2018:67(1):12-22.
[6] HIYOSHI T,FUJIWARA M,YAO Z. Postprandial hyperglycemia and postprandial hypertriglyceridemia in type 2 diabetes[J]. J Biomed Mater Res,2019,1:1-16.
[7]中华医学会糖尿病学分会(CDS)关于代谢综合征的建议[J].中华糖尿病杂志,2004,12:156-161.
[8] NIINA S,LEENA P,JAANA L,et al. Association of ADIPOQ gene variants with body weight,type 2 diabetes and serum adiponectin concentrations:The Finnish Diabetes Prevention Study[J]. BMC MED GENET,2011,12(5):13.
[9] WANG WL,ZHU H,XIE Y,et al. Relation between ADIPOQ gene polymorphisms and type 2 diabetes in a Chinese population[J]. Int J Clin Exp Med,2015,8(4):6124-6128.
[10]贾敏,高玉敏.人群脂联素基因多态性对2型糖尿病影响的研究进展[J].世界最新医学信息文摘,2018,8(1):86-88.
[11]邓露露,欧子豪,彭永正.米色脂肪细胞的诱导因子——抗肥胖的新策略[J].实用医学杂志,2017,33(19):3162-3165.
[12]王楚瑗,孔令芳,侯永生,等.脂联素与2型糖尿病进程的相关性[J].中国医科大学学报,2014,43(5):429-436.
[13] HOTTA K,FUNAHASHI T,A RITA Y,et al. Plasma concentrationof a novel,adipose-specific protein,adiponectin,in type2diabetic patients[J]. Arterioscler Thromb Vasc Biol,2000,20(6):1595-1599.
[14] DALAMAGA M,KARMANIOLAS K,CHAMBERLAND J,et al.Higher fetuin-A,lower adiponectin and free leptin levels mediate effects ofexcess body weight on insulin resistance and risk form yelodys plastic syndrome[J]. Metabolism,2013,62(12):1830-1839.
[15]王光亚,王霖霞,赵乃蕊,等. 2型糖尿病患者脂联素水平的变化及意义[J].河北医科大学学报,2015,36(9):1055-1058.
[16] ZHA D,WU X,GAO P. Adiponectin and its receptors in diabetic kidney disease:Molecular mechanisms and clinical potential[J]. Endocrinology,2017,158(7):2022-2034.
[17] CHEN L,LI L,CHEN J,et al. Oleoylethanolamide,anendogenous PPAR-Lig and,attenuates liver fibrosis targeting hepatic stellate cell[J]. Oncotarget,2015,6:42530-42540.
[18] YAMAUCHI T,IWABU M,OKADA M,et al. Adiponectin receptors:a review of their structure,function and how they work[J]. Best Pract Res Clin Endocrinol Metab,2014,28(1):15-23.
[19] SMEKAL A,VACLAVIK J. Adipokines and cardiovascular disease:A comprehensive review[J]. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub,2017,161(1):31-40.
[20] DREW B G,RYE K A,DUFFY S J,et al. The emerging role of HDL inglucose metabolism[J]. Nat Rev Endocrinol,2012,8(4):237-245.
[21] ECKARDSTEIN A,SIBLER RA. Possiblecontributions of lipoproteins and cholesterol to the pathogenesis of diabetes mellitus type 2[J]. Curr Opin Lipidol,2011,22(1):26-32.
[22] ABBASIA,CORPELEIJN E,GANSEVOORT R T,et al. Role of HDL choles-lerol and estimates of HDL particle compositionin future development of type2 diabetes in thegeneral population:The prevend Study[J]. J Clin Endocrinol Metab,2013,98(8):1352-1359.
[23]康庄,赵惠珠,苏恒,等.大理白族2型糖尿病与脂联素基因启动子SNPs的相关性[J].实用医学杂志,2013,29(15):2559-2561.
[24] JANICE S K,SOREN G,CLAUDIA H T,et al. Association of the PPARG Pro12Ala polymorphism with type 2 diabetes and incident coronary heart disease in a Hong Kong Chinese population[J]. Diabetes Res Clin PR,2012,97(3):483-491.
[25] WANG X,ZHANG S,CHEN Y,et al. APM1 gene variants-11377C/G and 4545G/C are associated respectively with obesity and with non-obesity in Chinese type 2 diabetes[J]. Diabetes Res Clin PR,2009,84(3):205-210.
[26]杜文聪,陆莹,叶新华,等.应用BP人工神经网络探讨脂联素基因多态性位点间交互作用与汉族人群2型糖尿病遗传易感性的关系[J].中国糖尿病杂志,2012,20(1):20-23.
[2] KARINA G,HUAIEN Z,ANOUAR H,et al. Decreased adiponectin-mediated signaling through the adipoR2 pathway is associated with carotid plaque instability[J]. STROKE,2017:1-22.
[3] LIU Y,SWEENEY G. Adiponectin action in skeletal muscle[J].Best Pract Res Clin Endocrinol Matal,2014,28(1):33-41.
[4] LINDBERG S,JENSEN J S,BJERRE M,et al. Adiponectin,type 2 diabetes and cardiovascular risk[J]. Eur J Prev Cardiol,2015:22(3):276-83.
[5] MENZAGHI C,TRISCHITTA V. The adiponectin paradox for allcause and cardiovascular mortality[J]. Diabetes,2018:67(1):12-22.
[6] HIYOSHI T,FUJIWARA M,YAO Z. Postprandial hyperglycemia and postprandial hypertriglyceridemia in type 2 diabetes[J]. J Biomed Mater Res,2019,1:1-16.
[7]中华医学会糖尿病学分会(CDS)关于代谢综合征的建议[J].中华糖尿病杂志,2004,12:156-161.
[8] NIINA S,LEENA P,JAANA L,et al. Association of ADIPOQ gene variants with body weight,type 2 diabetes and serum adiponectin concentrations:The Finnish Diabetes Prevention Study[J]. BMC MED GENET,2011,12(5):13.
[9] WANG WL,ZHU H,XIE Y,et al. Relation between ADIPOQ gene polymorphisms and type 2 diabetes in a Chinese population[J]. Int J Clin Exp Med,2015,8(4):6124-6128.
[10]贾敏,高玉敏.人群脂联素基因多态性对2型糖尿病影响的研究进展[J].世界最新医学信息文摘,2018,8(1):86-88.
[11]邓露露,欧子豪,彭永正.米色脂肪细胞的诱导因子——抗肥胖的新策略[J].实用医学杂志,2017,33(19):3162-3165.
[12]王楚瑗,孔令芳,侯永生,等.脂联素与2型糖尿病进程的相关性[J].中国医科大学学报,2014,43(5):429-436.
[13] HOTTA K,FUNAHASHI T,A RITA Y,et al. Plasma concentrationof a novel,adipose-specific protein,adiponectin,in type2diabetic patients[J]. Arterioscler Thromb Vasc Biol,2000,20(6):1595-1599.
[14] DALAMAGA M,KARMANIOLAS K,CHAMBERLAND J,et al.Higher fetuin-A,lower adiponectin and free leptin levels mediate effects ofexcess body weight on insulin resistance and risk form yelodys plastic syndrome[J]. Metabolism,2013,62(12):1830-1839.
[15]王光亚,王霖霞,赵乃蕊,等. 2型糖尿病患者脂联素水平的变化及意义[J].河北医科大学学报,2015,36(9):1055-1058.
[16] ZHA D,WU X,GAO P. Adiponectin and its receptors in diabetic kidney disease:Molecular mechanisms and clinical potential[J]. Endocrinology,2017,158(7):2022-2034.
[17] CHEN L,LI L,CHEN J,et al. Oleoylethanolamide,anendogenous PPAR-Lig and,attenuates liver fibrosis targeting hepatic stellate cell[J]. Oncotarget,2015,6:42530-42540.
[18] YAMAUCHI T,IWABU M,OKADA M,et al. Adiponectin receptors:a review of their structure,function and how they work[J]. Best Pract Res Clin Endocrinol Metab,2014,28(1):15-23.
[19] SMEKAL A,VACLAVIK J. Adipokines and cardiovascular disease:A comprehensive review[J]. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub,2017,161(1):31-40.
[20] DREW B G,RYE K A,DUFFY S J,et al. The emerging role of HDL inglucose metabolism[J]. Nat Rev Endocrinol,2012,8(4):237-245.
[21] ECKARDSTEIN A,SIBLER RA. Possiblecontributions of lipoproteins and cholesterol to the pathogenesis of diabetes mellitus type 2[J]. Curr Opin Lipidol,2011,22(1):26-32.
[22] ABBASIA,CORPELEIJN E,GANSEVOORT R T,et al. Role of HDL choles-lerol and estimates of HDL particle compositionin future development of type2 diabetes in thegeneral population:The prevend Study[J]. J Clin Endocrinol Metab,2013,98(8):1352-1359.
[23]康庄,赵惠珠,苏恒,等.大理白族2型糖尿病与脂联素基因启动子SNPs的相关性[J].实用医学杂志,2013,29(15):2559-2561.
[24] JANICE S K,SOREN G,CLAUDIA H T,et al. Association of the PPARG Pro12Ala polymorphism with type 2 diabetes and incident coronary heart disease in a Hong Kong Chinese population[J]. Diabetes Res Clin PR,2012,97(3):483-491.
[25] WANG X,ZHANG S,CHEN Y,et al. APM1 gene variants-11377C/G and 4545G/C are associated respectively with obesity and with non-obesity in Chinese type 2 diabetes[J]. Diabetes Res Clin PR,2009,84(3):205-210.
[26]杜文聪,陆莹,叶新华,等.应用BP人工神经网络探讨脂联素基因多态性位点间交互作用与汉族人群2型糖尿病遗传易感性的关系[J].中国糖尿病杂志,2012,20(1):20-23.