金银花与山银花绿原酸提取动力学比较研究
|
摘要
目的利用已建立的中药材成分封闭体系提取动力学模型,探讨金银花与山银花绿原酸的提取动力学差异。方法采用HPLC测定金银花与山银花绿原酸有效含量W0及分配系数ρ2,采用吸水法测定工艺参数V1、V2再计算V0,利用SPSS19.0软件非线性拟合得到参数M、N、L、α、β、π,用Excel2016按公式计算k、k1′、k2′、ρ1、t_(max)、c_(max)、AUC、P%、D%。运用总量统计矩相似度法计算两者的相似度。结果金银花的V0、V1、V2分别为18.69、9.50、30.20 m L,金银花绿原酸W0为3.75%、ρ2为0.884;山银花的V0、V1、V2分别为12.79、7.80、37.00 m L,山银花绿原酸W0为5.67%、ρ2为1.020。金银花与山银花绿原酸药时曲线呈三室模型,其主要提取动力学参数k分别为0.110 1、0.3755 h-1,k1′分别为3.632、3.288 h-1,k2′分别为53.12、55.28 h-1,ρ1分别为2.731、2.751,tmax分别为0.299 5、0.216 3 h,cmax分别为0.134 0、0.252 7 mg/m L,AUC分别为3.405、1.560 h,P%分别为35.73%、44.57%,D%分别为0.916 2%、2.680 7%。总量统计矩相似度为0.963 8,表明金银花与山银花绿原酸提取动力学行为基本相似。结论金银花与山银花绿原酸的溶出行为及其差异可以用中药提取动力学数学模型客观且有效地表达,本研究可以为进一步开展金银花与山银花提取工艺的优化及其制剂研究提供一定参考。
Objective To explore the extraction kinetic deviation of the chlorogenic acid(Ch A) in Lonicerae Japonicae Flos and Lonicerae Flos through established extract kinetic model of closed system of Chinese materia medica. Methods The content of Ch A(W0) and ρ2 in Lonicerae Japonicae Flos and Lonicerae Flos were determined by HPLC; The V1 and V2 were measured by water absorbing further to calculate V0; The value of M, N, L, α, β, and πwere estimated by curve fitting using SPSS19.0 edition; The extraction kinetic parameters k, k1′, k2′, ρ1, tmax, cmax, AUC, P%, and D% were calculated by Excel; their similarity was calculate by the total quantum statistical moment similarity(TQSMS). Results The V0, V1, and V2 for Lonicerae Japonicae Flos were 18.69, 9.50, and 30.20 m L, respectively. The W0 for the Ch A in Lonicerae Japonicae Flos were 3.75%, and ρ2 was 0.884; The V0, V1, and V2 for Lonicerae Flos were 12.79, 7.80, and 37.00 m L, respectively. The W0 for the Ch A in Lonicerae Flos were 5.67%, and ρ2 was 1.020; The extraction kinetic profiles for Ch A in Lonicerae Japonicae Flos and Lonicerae Flos were fitted three compartment model. The main kinetic parameters as k were 0.1101, 0.3755 h-1; k1′ were 3.632, 3.288 h-1; k2′ were 53.12, 55.28 h-1; ρ1 were 2.731, 2.751; tmax were 0.299 5, 0.216 3 h; cmax were 0.134 0, 0.252 7 mg/m L; AUC were 3.405, 1.560 h; P% were 35.73% and 44.57%; D% were 0.916 2% and 2.680 7%, respectively. Their TQSMS was 0.963 8, which indicated that the extraction kinetics of Ch A in Lonicerae Japonicae Flos and Lonicerae Flos had good similarity. Conclusion The extraction kinetic model described the dissolution behavior and deviation of extraction kinetic profiles for Ch A in Lonicerae Japonicae Flos and Lonicerae Flos objectively and effectively. This research can provide some references for further study on extraction processes and preparation of Lonicerae Japonicae Flos and Lonicerae Flos.
Objective To explore the extraction kinetic deviation of the chlorogenic acid(Ch A) in Lonicerae Japonicae Flos and Lonicerae Flos through established extract kinetic model of closed system of Chinese materia medica. Methods The content of Ch A(W0) and ρ2 in Lonicerae Japonicae Flos and Lonicerae Flos were determined by HPLC; The V1 and V2 were measured by water absorbing further to calculate V0; The value of M, N, L, α, β, and πwere estimated by curve fitting using SPSS19.0 edition; The extraction kinetic parameters k, k1′, k2′, ρ1, tmax, cmax, AUC, P%, and D% were calculated by Excel; their similarity was calculate by the total quantum statistical moment similarity(TQSMS). Results The V0, V1, and V2 for Lonicerae Japonicae Flos were 18.69, 9.50, and 30.20 m L, respectively. The W0 for the Ch A in Lonicerae Japonicae Flos were 3.75%, and ρ2 was 0.884; The V0, V1, and V2 for Lonicerae Flos were 12.79, 7.80, and 37.00 m L, respectively. The W0 for the Ch A in Lonicerae Flos were 5.67%, and ρ2 was 1.020; The extraction kinetic profiles for Ch A in Lonicerae Japonicae Flos and Lonicerae Flos were fitted three compartment model. The main kinetic parameters as k were 0.1101, 0.3755 h-1; k1′ were 3.632, 3.288 h-1; k2′ were 53.12, 55.28 h-1; ρ1 were 2.731, 2.751; tmax were 0.299 5, 0.216 3 h; cmax were 0.134 0, 0.252 7 mg/m L; AUC were 3.405, 1.560 h; P% were 35.73% and 44.57%; D% were 0.916 2% and 2.680 7%, respectively. Their TQSMS was 0.963 8, which indicated that the extraction kinetics of Ch A in Lonicerae Japonicae Flos and Lonicerae Flos had good similarity. Conclusion The extraction kinetic model described the dissolution behavior and deviation of extraction kinetic profiles for Ch A in Lonicerae Japonicae Flos and Lonicerae Flos objectively and effectively. This research can provide some references for further study on extraction processes and preparation of Lonicerae Japonicae Flos and Lonicerae Flos.
引文
[1]储茂泉,刘国杰.中药提取过程的动力学[J].药学学报,2002,37(7):559-562.
[2]贺福元,邓凯文,罗杰英,等.中药复方成分提取动力学数学模型的初步研究[J].中国中药杂志,2007,32(6):490-495.
[3]贺福元,邓凯文,杨大坚,等.中药材成分提取动力学数学模型的建立及参数分析[J].数理医药学杂志,2005,18(6):513-517.
[4]贺福元,邓凯文,吴德智,等.左金方与黄连中小檗碱型生物碱提取动力学差异性的研究[J].中成药,2009,31(9):1354-1359.
[5]国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015:30-31,221.
[6]林学政,柳春燕,陈靠山,等.不同地域牛蒡叶绿原酸的含量比较及其抑菌实验[J].天然产物研究与开发,2004,16(4):328-330.
[7]胡克杰,孙考祥,王璟璐,等.氯原酸体外抗病毒作用研究[J].哈尔滨医科大学学报,2001,35(6):430-432.
[8]张鞍灵,马琼,高锦明,等.绿原酸及其类似物与生物活性[J].中草药,2001,32(2):173-176.
[9]王强,陈东辉,邓文龙.金银花提取物对血脂与血糖的影响[J].中药药理与临床,2007,23(3):40-42.
[10]贺福元,贺红,邓凯文,等.借超分子“印迹模板”的自主作用解开金(山)银花纷争[J].中国中药杂志,2016,41(6):1152-1160.
[11]王震宇,陶洪斌,唐玉林.植物质外体的研究方法[J].植物生理学通讯,1999,35(5):394.
[12]贺福元,罗杰英,邓凯文.中药复方动力学数学模型-总量统计矩法的研究[J].世界科学技术-中医药现代化,2006,8(6):13-18.
[13]黄胜,贺福元,刘文龙,等.总量统计矩相似度法对左金缓释片多成分释放相似度的评价研究[J].中成药,2009,31(1):35-39.
[14]贺福元,邓凯文,黄胜,等.总量统计矩标准相似度数学模型的建立及应用研究[J].药学学报,2013,48(9):1453-1458.
[15]张丹,李章万,姜焱.HPLC测定金银花、菌陈及其10种中成药中绿原酸的含量[J].药物分析杂志,1996,16(2):83-85.
[2]贺福元,邓凯文,罗杰英,等.中药复方成分提取动力学数学模型的初步研究[J].中国中药杂志,2007,32(6):490-495.
[3]贺福元,邓凯文,杨大坚,等.中药材成分提取动力学数学模型的建立及参数分析[J].数理医药学杂志,2005,18(6):513-517.
[4]贺福元,邓凯文,吴德智,等.左金方与黄连中小檗碱型生物碱提取动力学差异性的研究[J].中成药,2009,31(9):1354-1359.
[5]国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015:30-31,221.
[6]林学政,柳春燕,陈靠山,等.不同地域牛蒡叶绿原酸的含量比较及其抑菌实验[J].天然产物研究与开发,2004,16(4):328-330.
[7]胡克杰,孙考祥,王璟璐,等.氯原酸体外抗病毒作用研究[J].哈尔滨医科大学学报,2001,35(6):430-432.
[8]张鞍灵,马琼,高锦明,等.绿原酸及其类似物与生物活性[J].中草药,2001,32(2):173-176.
[9]王强,陈东辉,邓文龙.金银花提取物对血脂与血糖的影响[J].中药药理与临床,2007,23(3):40-42.
[10]贺福元,贺红,邓凯文,等.借超分子“印迹模板”的自主作用解开金(山)银花纷争[J].中国中药杂志,2016,41(6):1152-1160.
[11]王震宇,陶洪斌,唐玉林.植物质外体的研究方法[J].植物生理学通讯,1999,35(5):394.
[12]贺福元,罗杰英,邓凯文.中药复方动力学数学模型-总量统计矩法的研究[J].世界科学技术-中医药现代化,2006,8(6):13-18.
[13]黄胜,贺福元,刘文龙,等.总量统计矩相似度法对左金缓释片多成分释放相似度的评价研究[J].中成药,2009,31(1):35-39.
[14]贺福元,邓凯文,黄胜,等.总量统计矩标准相似度数学模型的建立及应用研究[J].药学学报,2013,48(9):1453-1458.
[15]张丹,李章万,姜焱.HPLC测定金银花、菌陈及其10种中成药中绿原酸的含量[J].药物分析杂志,1996,16(2):83-85.