山银花提取物定性定量方法考察
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摘要
目的:对山银花提取物进行定性定量研究,为其质量控制提供依据。方法:采用Acclaim 120 C18色谱柱(250 mm×4.6 mm,5μm),乙腈-0.4%磷酸溶液为流动相进行梯度洗脱,流速为1.0 ml·min-1,检测波长327 nm,柱温为25℃,测定绿原酸的含量;并以绿原酸为参照物,通过特征图谱鉴别6种有机酸。同时采用HPLC法对灰毡毛忍冬皂苷乙进行定性鉴别。结果:绿原酸在23.61~141.65μg·ml-1范围内线性关系良好(r=0.999 8),回收率为98.8%,RSD 1.4%(n=6)。灰毡毛忍冬皂苷乙为山银花提取物中的特征性成分,专属性强。结论:本法简便、可靠、准确,可用于山银花提取物的质量控制。
Objective: To establish qualitative and quantitative methods for Lonicerae Flos extract to provide basis for the quality control. Methods: An Acclaim 120 C18 column( 250 mm × 4. 6 mm,5 μm) was used at the temperature of 25℃. The mobile phase consisted of acetonitril and 0. 4% phosphoric acid solution with gradient elution. The flow rate was 1. 0 ml·min-1,the detection wavelength was 327 nm and the column temperature was 25℃. Chlorogenic acid was determined quantitatively and 6 organic acids were detected qualitatively. Dipsacoside B was identified by HPLC as well. Results: Chlorogenic acid had a good linear correlation within the range of 23. 61-141. 65 μg·ml-1( r = 0. 999 8). The average recovery was 98. 8%,and the RSD was 1. 4%( n = 6). Dipsacoside B was the exclusive content of Lonicerae Flos extract. Conclusion: The method is simple,reliable and accurate,and can be used for the quality control for Lonicerae Flos extract.
Objective: To establish qualitative and quantitative methods for Lonicerae Flos extract to provide basis for the quality control. Methods: An Acclaim 120 C18 column( 250 mm × 4. 6 mm,5 μm) was used at the temperature of 25℃. The mobile phase consisted of acetonitril and 0. 4% phosphoric acid solution with gradient elution. The flow rate was 1. 0 ml·min-1,the detection wavelength was 327 nm and the column temperature was 25℃. Chlorogenic acid was determined quantitatively and 6 organic acids were detected qualitatively. Dipsacoside B was identified by HPLC as well. Results: Chlorogenic acid had a good linear correlation within the range of 23. 61-141. 65 μg·ml-1( r = 0. 999 8). The average recovery was 98. 8%,and the RSD was 1. 4%( n = 6). Dipsacoside B was the exclusive content of Lonicerae Flos extract. Conclusion: The method is simple,reliable and accurate,and can be used for the quality control for Lonicerae Flos extract.
引文
1中国药典[S].一部.2015年版.30-31,1496-1497
2 李丹,李会军,高雯,等.一测多评法测定山银花提取物中6种绿原酸类成分[J].中国中药杂志,2014,39(6):2305-2310
3 郑荣,郏征伟,王柯,等.金银花提取物中6种有机酸类成分的测定[J].中成药,2013,35(3):560-564
4 曹冬红,张贺文,杨科,等.高效液相色谱法测定山银花中绿原酸的含量[J].中南药学,2007,5(1):36-38
5 姚正颖,张卫明,李春霞,等.不同干燥方法对灰毡毛忍冬花蕾中活性成分含量的影响[J].中国野生植物资源,2013,32(2):8-11
6 任琰,钟根秀,于婧怡.出口植物提取物质量安全现状及监管对策[J].浙江农业科学,2016,57(12):1956-1959,1967
2 李丹,李会军,高雯,等.一测多评法测定山银花提取物中6种绿原酸类成分[J].中国中药杂志,2014,39(6):2305-2310
3 郑荣,郏征伟,王柯,等.金银花提取物中6种有机酸类成分的测定[J].中成药,2013,35(3):560-564
4 曹冬红,张贺文,杨科,等.高效液相色谱法测定山银花中绿原酸的含量[J].中南药学,2007,5(1):36-38
5 姚正颖,张卫明,李春霞,等.不同干燥方法对灰毡毛忍冬花蕾中活性成分含量的影响[J].中国野生植物资源,2013,32(2):8-11
6 任琰,钟根秀,于婧怡.出口植物提取物质量安全现状及监管对策[J].浙江农业科学,2016,57(12):1956-1959,1967