不同植物来源绿原酸的提取工艺优化
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摘要
为优化绿原酸的提取工艺,试验对比了不同的提取溶剂、溶液浓度、提取温度、提取时间、料液比等因素对金银花、山银花、杜仲叶和烟叶中绿原酸提取率的影响。试验结果表明,最佳工艺优化方案为:金银花、山银花、杜仲叶的绿原酸提取溶剂为60%乙醇,烟叶中绿原酸提取溶剂为蒸馏水;提取温度60℃,料液比1∶16,提取时间60 min。在此条件下,金银花、山银花、杜仲叶、烟叶中的绿原酸提取率最高分别可达6.05%、6.01%、2.07%、2.33%。该提取工艺确保提取物成分与其天然状态相近,有利于进一步的纯化。
To explore the effects of extraction rate of chlorogenic acid in Honeysuckle, Flos Lonicerae, Eucommia ulmoides Oliver leaves and tobacco leaves, in different extraction solvents, solution concentration, extraction temperature, extraction time, solid-liquid ratio and to find the best extraction process. Through experiment and data analysis, the chlorogenic acid extraction conditions of Honeysuckle, Flos Lonicerae and Eucommia ulmoides Oliver leaves as follows: 60% ethanol solvent, extracting temperature 60 ℃, solid-liquid ratio 1:16,extracting time 60 min; and chlorogenic acid in tobacco leaf extract solvent is distilled water. Under these conditions, the extraction rates of chlorogenic acid in Honeysuckle, Flos Lonicerae. Eucommia ulmoides Oliver leaves and tobacco leaves reached the highest: 6.05%, 6.01%,2.07% and 2.33% respectively. The extraction process ensures that the components of the extract are similar to its natural state and lays a foundation for further purification.
To explore the effects of extraction rate of chlorogenic acid in Honeysuckle, Flos Lonicerae, Eucommia ulmoides Oliver leaves and tobacco leaves, in different extraction solvents, solution concentration, extraction temperature, extraction time, solid-liquid ratio and to find the best extraction process. Through experiment and data analysis, the chlorogenic acid extraction conditions of Honeysuckle, Flos Lonicerae and Eucommia ulmoides Oliver leaves as follows: 60% ethanol solvent, extracting temperature 60 ℃, solid-liquid ratio 1:16,extracting time 60 min; and chlorogenic acid in tobacco leaf extract solvent is distilled water. Under these conditions, the extraction rates of chlorogenic acid in Honeysuckle, Flos Lonicerae. Eucommia ulmoides Oliver leaves and tobacco leaves reached the highest: 6.05%, 6.01%,2.07% and 2.33% respectively. The extraction process ensures that the components of the extract are similar to its natural state and lays a foundation for further purification.
引文
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[14]Hu W,Guo T,Jiang W J,et al.Effects of ultrahigh pressure extraction on yield and antioxidant activity of chlorogenic acid and cynaroside extracted from flower buds of Lonicera japonica[J].Chinese Journal of Natural Medicines,2015,13(6):445-453.
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[16]Liu T,Sui X,Li L,et al.Application of ionic liquids based enzymeassisted extraction of chlorogenic acid from Eucommia ulmoides leaves[J].Analytical Chimica Acta,2016(903):91-99.
[17]Wu S,Liu Q A,Wang W,et al.Quality control in liquid-liquid extraction of Reduning injection using near-infrared spectroscopy technology[J].Zhongguo Zhong Yao Za Zhi,2015,40(3):437-442.
[18]Yao X H,Xu J Y,Hao J Y,et al.Microwave assisted extraction for the determination of chlorogenic acid in Flos Lonicerae by direct analysis in real time mass spectrometry(DART-MS)[J].Journal of Chromatography B,2018(1092):82-87.
[19]王晴晴,张盛,李银花,等.HPLC同时测定花生壳中绿原酸3,4-二咖啡酰奎尼酸和木犀草素[J].农产品加工,2011(6):69-70,78.
[20]石嘉怿,张英.高效液相色谱法测定青梅花、枝、叶中绿原酸类化合物[J].食品科学,2011,32(2):191-194.
[21]席利莎,木泰华,孙红男.绿原酸类物质的国内外研究进展[J].核农学报,2014,28(2):292-301.
[22]刘颖,郭明晔,白根本.绿原酸的研究进展[J].中药材,2012,35(7):1180-1185.
[2]李玉山.绿原酸在天然植物中的分布和提取纯化工艺研究进展[J].解放军药学学报,2012,28(4):355-359.
[3]梁萱,李嘉会,梁永锋.桃花中绿原酸抗氧化活性的研究[J].中国现代中药,2018(6):697-701.
[4]Rui L,Xie M,Hu B,et al.Enhanced solubility and antioxidant activity of chlorogenic acid-chitosan conjugates due to the conjugation of chitosan with chlorogenic acid[J].Carbohydr Polym,2017(170):206-216.
[5]胡居吾,韩晓丹,付建平,等.三种绿原酸提取物的抑菌和抗氧化效果比较[J].天然产物研究与开发,2017,29(11):1928-1933.
[6]杨晓丽,张君利,王京峰,等.绿原酸抗肿瘤作用及机制研究进展[J].中国实验方剂学杂志,2018,24(19):229-234.
[7]Yamagata K,Izawa Y,Onodera D,et al.Chlorogenic acid regulates apoptosis and stem cell marker-related gene expression in A549 human lung cancer cells[J].Molecular and Cell Biochemistry,2017,441(1-2):9-19.
[8]Gong X,Luan Q,Zhou X,et al.UHPLC-ESI-MS/MS determination and pharmacokinetics of pinoresinol glucoside and chlorogenic acid in rat plasma after oral administration of Eucommia ulmoides Oliv extract[J].Biomedical Chromatography,2017,31(11),e4008.
[9]Huang Y,Chen H,Zhou X,et al.Inhibition effects of chlorogenic acid on benign prostatic hyperplasia in mice[J].European Journal of Pharmacology,2017(809):191-195.
[10]赵金娟,戴雪梅,曲永胜,等.绿原酸药效学研究进展[J].中国野生植物资源,2013,32(4):1-5.
[11]赵琰玲,尹莲.金银花化学成分与有效成分提取研究进展[J].医药导报,2007,26(5):521-523.
[12]周惠燕,陈珏.金银花提取物中绿原酸含量测定[J].中国中医药信息杂志,2011,18(11):61-62.
[13]刘亚敏,刘玉民,李琼,等.超声波辅助提取山银花绿原酸工艺及其抗氧化性研究[J].食品工业科技,2014,35(1):186-190,195.
[14]Hu W,Guo T,Jiang W J,et al.Effects of ultrahigh pressure extraction on yield and antioxidant activity of chlorogenic acid and cynaroside extracted from flower buds of Lonicera japonica[J].Chinese Journal of Natural Medicines,2015,13(6):445-453.
[15]Wianowska D,Typek R,Dawidowicz A L.Chlorogenic acid stability in pressurized liquid extraction conditions[J].Journal of AOACInternational,2015,98(2):415-421.
[16]Liu T,Sui X,Li L,et al.Application of ionic liquids based enzymeassisted extraction of chlorogenic acid from Eucommia ulmoides leaves[J].Analytical Chimica Acta,2016(903):91-99.
[17]Wu S,Liu Q A,Wang W,et al.Quality control in liquid-liquid extraction of Reduning injection using near-infrared spectroscopy technology[J].Zhongguo Zhong Yao Za Zhi,2015,40(3):437-442.
[18]Yao X H,Xu J Y,Hao J Y,et al.Microwave assisted extraction for the determination of chlorogenic acid in Flos Lonicerae by direct analysis in real time mass spectrometry(DART-MS)[J].Journal of Chromatography B,2018(1092):82-87.
[19]王晴晴,张盛,李银花,等.HPLC同时测定花生壳中绿原酸3,4-二咖啡酰奎尼酸和木犀草素[J].农产品加工,2011(6):69-70,78.
[20]石嘉怿,张英.高效液相色谱法测定青梅花、枝、叶中绿原酸类化合物[J].食品科学,2011,32(2):191-194.
[21]席利莎,木泰华,孙红男.绿原酸类物质的国内外研究进展[J].核农学报,2014,28(2):292-301.
[22]刘颖,郭明晔,白根本.绿原酸的研究进展[J].中药材,2012,35(7):1180-1185.