消银颗粒和开玄补肾方对咪喹莫特诱导的银屑病样小鼠模型的影响
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摘要
目的观察比较消银颗粒和开玄补肾方对银屑病小鼠模型的干预作用。方法将30只BALB/c雄性小鼠随机分为空白组、模型组、消银颗粒组、开玄补肾方组和甲氨蝶呤组。实验期间每天观察银屑病样小鼠皮损变化,对小鼠背部皮肤进行拍照,并进行银屑病皮损面积和严重程度指数(PASI)评分;造模后第7 d取小鼠背部皮肤组织,HE染色观察皮肤组织病理学改变并检测表皮厚度;免疫组化法检测皮损表皮中增殖细胞核抗原(PCNA)的表达。结果根据模型组小鼠红斑、鳞屑、浸润评分及其肉眼观察,模型组小鼠皮损与银屑病样皮损类似,造模成功;在2～7 d时,开玄补肾组、消银颗粒组、甲氨蝶呤组各项PASI评分均低于模型组小鼠,在4～6 d时,PASI评分显著降低,差异有统计学意义(P <0. 05),其中消银颗粒组小鼠鳞屑评分与开玄补肾方组相比下降更明显(P <0. 05或P <0. 01),而开玄补肾方组小鼠皮损红斑评分与消银颗粒组小鼠相比下降更显著,差异有统计学意义(P <0. 05); HE和PCNA染色显示,消银颗粒组、开玄补肾方组和甲氨蝶呤组小鼠表皮厚度显著减少,炎症细胞浸润得到抑制,表皮着色细胞层数减少(P <0. 01),其中开玄补肾方组小鼠表皮厚度、表皮着色细胞层数与消银颗粒组相比减少趋势更加明显。结论消银颗粒和开玄补肾方可以减少小鼠银屑病样皮损、降低表皮厚度并抑制表皮细胞过度增殖,但开玄补肾方对于皮损浸润肥厚的血瘀型斑块状银屑病具有较好的改善作用,而消银颗粒对于鳞屑较多的风燥型银屑病具有较好的改善作用。
Objective To observe and compare the intervention effect of Xiaoyin granule and Kaixuan Bushen recipe on psoriasis mice model. Method 30 BALB/c male mouse are randomly divided into blank group,model group,Xiaoyin granules group,Kaixuan Bushen recipe group and methotrexate group. During the experiment,the skin lesions of psoriasis-like mice were observed daily,and the skin of the back of the mice was photographed,and the psoriasis lesion area and severity index( PASI) scores were scored. The skin tissues of the back of the mice were taken on the 7th day after modeling,and the histopathological changes of the skin were observed and the thickness of the epidermis was detected by HE staining. The expression of proliferating cell nuclear antigen( PCNA) in epidermis by immunohistochemical method was used. According to the scores of erythema,scales,infiltration and naked eye observation,the skin lesions of model mice were similar to those of psoriasis-like lesions. Result At 2 ～ 7 days,the PASI scores of Kaixuan Bushen group,Xiaoyin granule group and methotrexate group were lower than those of model group. At 4 ～ 6 days,the PASI scores of Xiaoyin granule group were significantly lower than those of Kaixuan Bushen recipe group( P < 0. 05),and the scale scores of mice in Kaixuan Bushen recipe group were significantly lower than those in Kaixuan Bushen recipe group( P < 0. 05 or 0. 01). Compared with mice in Xiaoyin granule group,the erythema score of skin lesion decreased more significantly( P < 0. 05). HE and PCNA staining showed that the epidermal thickness of mice in Xiaoyin granule group,Kaixuan Bushen recipe group and methotrexate group decreased significantly,the infiltration of inflammatory cells was inhibited,and the number of epidermal staining cell layers decreased( P < 0. 01). The epidermal thickness and the number of epidermal staining cell layers in Kaixuan Bushen recipe group decreased obviously compared with Xiaoyin granule group. Conclusion Xiaoyin granule and Kaixuan Bushen recipe can reduce psoriasis-like skin lesions,reduce epidermal thickness and inhibit excessive proliferation of epidermal cells in mice,but Kaixuan Bushen recipe has a good improvement effect on plaque psoriasis of blood stasis type with hypertrophic skin lesions,and Xiaoyin granule has a good improvement on wind-drying psoriasis with more scales.
Objective To observe and compare the intervention effect of Xiaoyin granule and Kaixuan Bushen recipe on psoriasis mice model. Method 30 BALB/c male mouse are randomly divided into blank group,model group,Xiaoyin granules group,Kaixuan Bushen recipe group and methotrexate group. During the experiment,the skin lesions of psoriasis-like mice were observed daily,and the skin of the back of the mice was photographed,and the psoriasis lesion area and severity index( PASI) scores were scored. The skin tissues of the back of the mice were taken on the 7th day after modeling,and the histopathological changes of the skin were observed and the thickness of the epidermis was detected by HE staining. The expression of proliferating cell nuclear antigen( PCNA) in epidermis by immunohistochemical method was used. According to the scores of erythema,scales,infiltration and naked eye observation,the skin lesions of model mice were similar to those of psoriasis-like lesions. Result At 2 ～ 7 days,the PASI scores of Kaixuan Bushen group,Xiaoyin granule group and methotrexate group were lower than those of model group. At 4 ～ 6 days,the PASI scores of Xiaoyin granule group were significantly lower than those of Kaixuan Bushen recipe group( P < 0. 05),and the scale scores of mice in Kaixuan Bushen recipe group were significantly lower than those in Kaixuan Bushen recipe group( P < 0. 05 or 0. 01). Compared with mice in Xiaoyin granule group,the erythema score of skin lesion decreased more significantly( P < 0. 05). HE and PCNA staining showed that the epidermal thickness of mice in Xiaoyin granule group,Kaixuan Bushen recipe group and methotrexate group decreased significantly,the infiltration of inflammatory cells was inhibited,and the number of epidermal staining cell layers decreased( P < 0. 01). The epidermal thickness and the number of epidermal staining cell layers in Kaixuan Bushen recipe group decreased obviously compared with Xiaoyin granule group. Conclusion Xiaoyin granule and Kaixuan Bushen recipe can reduce psoriasis-like skin lesions,reduce epidermal thickness and inhibit excessive proliferation of epidermal cells in mice,but Kaixuan Bushen recipe has a good improvement effect on plaque psoriasis of blood stasis type with hypertrophic skin lesions,and Xiaoyin granule has a good improvement on wind-drying psoriasis with more scales.
引文
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[7]周杰.从肺论治寻常型银屑病血虚风燥证的临床研究[D].长沙:湖南中医药大学,2017.
[8]刘红华,魏国奇,单筠筠.消银颗粒联合西药治疗血热型银屑病60例观察[J].浙江中医杂志,2016,51(12):924.
[9]庄晨,宋坪主任医师运用“开通玄府、补肾培元”法治疗银屑病经验总结及机理初探[D].北京:北京中医药大学,2015.
[10]宋坪,王晓旭,杨茂誉,等.开通玄府、通络解毒法治疗斑块状银屑病120例疗效观察[J].中医杂志,2013,54(17):1476-1479.
[2] VALINS W,AMINI S,BERMAN B. The expression of Toll-like receptors in dermatological diseases and the therapeu-tic effect of current and newer topical Toll-like receptormodulators[J]. J Clin Aesthet Dermatol,2010,3(9):20-29.
[3]底婷婷,赵京霞,王燕,等.凉血活血胶囊对咪喹莫特诱导小鼠银屑病样皮损的干预作用[J].中国病理生理杂志,2012,28(4):718-722.
[4]赵京霞,底婷婷,王燕,等. IL-23/IL-17炎症轴在咪喹莫特诱导的小鼠银屑病样皮肤损害中的作用[J].中国病理生理杂志,2013,29(6):1086-1094.
[5]胡学军,梁鸿健,关日邦.甲氨蝶呤治疗系统性红斑狼疮的研究[J].中国基层医药,2009,16(4):598-590.
[6]刘瓦利,王俊慧,崔炳南,等.寻常型银屑病中医辨证论治规律探讨[J].北京中医药,2014,33(4):269-270.
[7]周杰.从肺论治寻常型银屑病血虚风燥证的临床研究[D].长沙:湖南中医药大学,2017.
[8]刘红华,魏国奇,单筠筠.消银颗粒联合西药治疗血热型银屑病60例观察[J].浙江中医杂志,2016,51(12):924.
[9]庄晨,宋坪主任医师运用“开通玄府、补肾培元”法治疗银屑病经验总结及机理初探[D].北京:北京中医药大学,2015.
[10]宋坪,王晓旭,杨茂誉,等.开通玄府、通络解毒法治疗斑块状银屑病120例疗效观察[J].中医杂志,2013,54(17):1476-1479.