湿地植物再力花对铬胁迫的生理生化响应
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摘要
在小型模拟人工湿地实验系统中,用不同浓度梯度(0、5、10、20、40、60 mg/L)的含铬废水对再力花进行污染胁迫,研究铬胁迫下湿地植物再力花的生理生化指标[如丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化物酶(POD)和根系活力(TTC)等]响应,为其在污染环境中作为水体环境修复植物的可行性提供科学依据。结果表明,随着铬胁迫浓度的增加,沙基质及其再力花体内的铬含量都随着胁迫浓度的增加而增加;叶片中叶绿素a、b及其总量均呈现先增后减,然后再增加的趋势;脯氨酸(Pro)表现为先降低后升高;谷胱甘肽(GSH)、MDA、超氧阴离子自由基、苯丙氨酸解氨酶(PAL)均总体呈现增加趋势;在铬浓度10 mg/L后,SOD和POD含量增加,而TTC和过氧化氢酶(CAT)随铬浓度的增加而降低。综上,再力花能够在铬胁迫下对其生理生化特性作出相应调节,具有较强的适应力和耐污力,可作为人工湿地处理含铬废水的湿地植物之一。
In a small simulated constructed wetland experimental system,the pollution Cr stress was applied to the Thalia dealbata with the chromium-containing wastewater with different concentration gradients,i.e.0,5,10,20,40,60 mg/L.The contents of malt aldehyde(MDA),superoxide dismutase(SOD),peroxidase(POD),root activity(TTC) and other physiological and biochemical characteristics of wetland plants under Cr stress were determined.The impact of the characteristics provides a scientific basis for its feasibility of repairing plants as a water environment in a polluted environment.The results showed that with the increase of Cr stress concentration,the Cr content in the sand matrix and its Thalia dealbata increased.The chl a,b and total amount in the leaves increased first and then decreased,then the trend of increasing.The proline(Pro) appears to decrease first and then increase,and Glutathione(GSH),MDA,superoxide anion free radicals,and phenylalanine ammonia lyase(PAL) all showed an increasing trend.After Cr concentration of 10 mg/L,SOD and POD increased,while TTC and Hydrogenase(CAT) decreases as the concentration of Cr increases.In summary,Thalia dealbata can adjust its physiological and biochemical characteristics under Cr stress,and has strong adaptability and stain resistance.It can be used as one of the wetland plants for treating Cr-containing wastewater in constructed wetlands.
In a small simulated constructed wetland experimental system,the pollution Cr stress was applied to the Thalia dealbata with the chromium-containing wastewater with different concentration gradients,i.e.0,5,10,20,40,60 mg/L.The contents of malt aldehyde(MDA),superoxide dismutase(SOD),peroxidase(POD),root activity(TTC) and other physiological and biochemical characteristics of wetland plants under Cr stress were determined.The impact of the characteristics provides a scientific basis for its feasibility of repairing plants as a water environment in a polluted environment.The results showed that with the increase of Cr stress concentration,the Cr content in the sand matrix and its Thalia dealbata increased.The chl a,b and total amount in the leaves increased first and then decreased,then the trend of increasing.The proline(Pro) appears to decrease first and then increase,and Glutathione(GSH),MDA,superoxide anion free radicals,and phenylalanine ammonia lyase(PAL) all showed an increasing trend.After Cr concentration of 10 mg/L,SOD and POD increased,while TTC and Hydrogenase(CAT) decreases as the concentration of Cr increases.In summary,Thalia dealbata can adjust its physiological and biochemical characteristics under Cr stress,and has strong adaptability and stain resistance.It can be used as one of the wetland plants for treating Cr-containing wastewater in constructed wetlands.
引文
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7付佳佳,吴亮.再力花对铜污染环境修复潜力及生态治理研究进展.广东蚕业,2017;51(1):42-43Fu Jiajia,Wu Liang.Advances in research on remediation potential and ecological management of copper pollution.Guangdong Sericulture,2017;51(1):42-43
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