纳米膜修饰长周期光纤光栅生物传感特性研究
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摘要
针对长周期光纤光栅用作折射率传感器时对低浓度溶液不敏感的问题,提出了一种纳米膜修饰的光栅生物传感器,实现了抗原生物分子的低浓度测量。实验结果表明,经薄膜修饰后的光栅传感器,对低浓度的抗原溶液探测的灵敏度有了显著提高。当羊抗兔IgG浓度(质量浓度)为固定值0.01mg·mL~(-1)时,随着兔IgG浓度的增加,覆膜长周期光纤光栅峰值损耗响应明显。实验测得周期为445,460,500μm的生物传感器对应的浓度灵敏度分别为2101.5,1306.5,575.9dB·mg~(-1)·mL。待测抗原浓度仅与光栅峰值损耗有关,实验测得兔IgG最小浓度为0.0003125mg·mL~(-1)。该传感器灵敏度高、无需标记、稳定性强,且结构简单不易受电磁干扰,在生物传感领域拥有广阔的应用前景。
We propose a nanofilm-modified grating biosensor that is able to detect low-concentration antigen biomolecules in order to find a solution for the insensitivity problem of the long-period fiber gratings(LPFGs)when used as refractive index sensors to detect low-concentration solutions.The experimental results show that the sensitivity of this biosensor is significantly improved.When the concentration of goat anti-rabbit IgG is fixed to0.01 mg·mL-1,the peak loss of the LPFG of the film indicates a significant response to the increase in rabbit IgG concentrations.Herein,the concentration is defined as a mass concentration.The biosensors successfully achieve concentration sensitivities of 2101.5,1306.5,and 575.9 dB·mg-1·mL measured at 445,460,and 500μm,respectively.Furthermore,the concentration of the antigen is related to the peak loss of the grating.The lowest detectable concentration of the rabbit IgG is 0.0003125 mg·mL-1.Given the high sensitivity,free labeling,high stability,simple structure,and immunity from the electromagnetic interference,the sensor promises an excellent application prospect in the biosensing field.
We propose a nanofilm-modified grating biosensor that is able to detect low-concentration antigen biomolecules in order to find a solution for the insensitivity problem of the long-period fiber gratings(LPFGs)when used as refractive index sensors to detect low-concentration solutions.The experimental results show that the sensitivity of this biosensor is significantly improved.When the concentration of goat anti-rabbit IgG is fixed to0.01 mg·mL-1,the peak loss of the LPFG of the film indicates a significant response to the increase in rabbit IgG concentrations.Herein,the concentration is defined as a mass concentration.The biosensors successfully achieve concentration sensitivities of 2101.5,1306.5,and 575.9 dB·mg-1·mL measured at 445,460,and 500μm,respectively.Furthermore,the concentration of the antigen is related to the peak loss of the grating.The lowest detectable concentration of the rabbit IgG is 0.0003125 mg·mL-1.Given the high sensitivity,free labeling,high stability,simple structure,and immunity from the electromagnetic interference,the sensor promises an excellent application prospect in the biosensing field.
引文
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[16]Liu J M.Based on the research of optical fiber biosensor[J].Wireless Internet Technology,2015(5):118-120.刘江明.基于光纤维生物传感器的研究[J].无线互联科技,2015(5):118-120.
[17]Wang Y M,Zhang Y Y,Pang X F,et al.Study on microscopic composition of fiber-optic after silanization by scanning electron microscopy and energy dispersive spectrometry[J].Journal of Instrumental Analysis,2008,27(11):1244-1247.王以明,张玉玉,庞小峰,等.硅烷化光纤表面微结构成分的扫描电镜及能谱仪联用表征[J].分析测试学报,2008,27(11):1244-1247.
[18]Jiang R,Shan L,Wang X G.Study on the immobilization technology of bromelin with attapulgite modified by 3-aminopropyltriethoxysilane[J].Journal of Anhui Agricultural Sciences,2008,36(25):10739-10741.蒋荣,单良,王兴国.3-氨基丙基三乙氧基硅烷改性凹凸棒土固定化菠萝蛋白酶工艺研究[J].安徽农业科学,2008,36(25):10739-10741.
[2]Guo T.Review on plasmonic optical fiber grating biosensors[J].Acta Optica Sinica,2018,38(3):0328006.郭团.等离子体共振光纤光栅生物传感器综述[J].光学学报,2018,38(3):0328006.
[3]Li Q S,Shi J G,Wang S J,et al.Research advance of the application of long-period fiber grating in biological and chemical sensors[J].Shandong Science,2010,23(4):36-42,50.李秋顺,史建国,王少杰,等.长周期光纤光栅在生物和化学传感器领域研究进展[J].山东科学,2010,23(4):36-42,50.
[4]Wang H,Zhou W C,Li K W,et al.Label-free biosensing characteristics of micro/nano-fiber coupler[J].Acta Optica Sinica,2017,37(3):0306005.汪海,周文超,李凯伟,等.微纳光纤耦合器无标生物传感特性[J].光学学报,2017,37(3):0306005.
[5]Liu H Y,Liang D K,Zeng J,et al.Long period fiber grating refractive index sensitivity-based reinforcing rebar corrosion sensor in concrete[J].Acta Optica Sinica,2011,31(8):0806009.刘宏月,梁大开,曾捷,等.基于长周期光纤光栅折射率敏感特性的混凝土结构钢筋锈蚀监测[J].光学学报,2011,31(8):0806009.
[6]Wang J Y,Tong Z R,Yang X F,et al.Simultaneous measurement of temperature and refraction index based on multimode interference and long-period fiber grating[J].Chinese Journal of Lasers,2012,39(9):0905003.王洁玉,童峥嵘,杨秀峰,等.基于多模干涉和长周期光纤光栅的温度及折射率同时测量[J].中国激光,2012,39(9):0905003.
[7]Zhang F,Li Q S,Yao W G,et al.Applications and progress of nanofilm-modified long period fiber grating in biological and chemical analysis[J].Chinese Optics,2014,7(1):57-67.张帆,李秋顺,姚卫国,等.覆膜长周期光纤光栅在生化分析中的应用及研究进展[J].中国光学,2014,7(1):57-67.
[8]Zhou W P,Wang S T,Yu Y C,et al.Research progress in fabrication of embedded microball lenses,energy devices and biosensors by femtosecond laser direct writing[J].Chinese Journal of Lasers,2017,44(1):0102002.周伟平,王树同,于泳超,等.飞秒激光直写制备内嵌微透镜、能源器件及生物传感器的研究进展[J].中国激光,2017,44(1):0102002.
[9]Zhang Z R,Zhang G M,Zhang X P.Design and simulation of biosensor based on long period fiber grating[J].Chinese Journal of Sensors and Actuators,2008,21(6):916-919.张志荣,张冠茂,张晓萍.基于长周期光纤光栅的生物传感器设计及仿真[J].传感技术学报,2008,21(6):916-919.
[10]Zhuang Q R,Gong D M,Zhang W B,et al.Long period fiber grating biosensor for detecting bio-layer thickness[J].Acta Laser Biology Sinica,2006,15(1):106-110.庄其仁,龚冬梅,张渭滨,等.长周期光纤光栅生物膜传感器[J].激光生物学报,2006,15(1):106-110.
[11]Fang Z,Ju W,Qin Y N,et al.Preparation and evaluation of FITC labeled Escherichia coli O157:H7polyclonal antibody[J].Journal of Jilin University(Medicine Edition),2013,39(1):165-169.方珍,鞠文,秦亚楠,等.大肠杆菌O157:H7异硫氰酸荧光素标记抗体的制备及评价[J].吉林大学学报(医学版),2013,39(1):165-169.
[12]Shi G M,Yang Y,Wang Y L.Research progress of amperometric immobilized biosensor[J].The Science and Technology of Gelatin,2012,32(2):53-55,59.史光明,杨寅,王昱琳.电流型固定化酶生物传感器的研究进展[J].明胶科学与技术,2012,32(2):53-55,59.
[13]Chao K F,Zhang Y L,Kong X G,et al.A study on fluorescence spectroscopy of immobilizing FITClabeled antibody between fiber optic surface and biomolecule[J].Chinese Journal of Luminescence,2007,28(6):940-944.朝克夫,张友林,孔祥贵,等.光纤与生物分子界面上固定FITC标记抗体的荧光光谱[J].发光学报,2007,28(6):940-944.
[14]Zhang X E.Biological sensor[M].Beijing:Chemical Industry Press,2006:16-30.张先恩.生物传感器[M].北京:化学工业出版社,2006:16-30.
[15]Zhang Y B,Xu C,Yao H,et al.Surface plasmon resonance biosensor and its application[J].Journal of Analytical Science,2012,28(1):126-132.张延彪,徐超,姚辉,等.表面等离子体共振生物传感器及其应用[J].分析科学学报,2012,28(1):126-132.
[16]Liu J M.Based on the research of optical fiber biosensor[J].Wireless Internet Technology,2015(5):118-120.刘江明.基于光纤维生物传感器的研究[J].无线互联科技,2015(5):118-120.
[17]Wang Y M,Zhang Y Y,Pang X F,et al.Study on microscopic composition of fiber-optic after silanization by scanning electron microscopy and energy dispersive spectrometry[J].Journal of Instrumental Analysis,2008,27(11):1244-1247.王以明,张玉玉,庞小峰,等.硅烷化光纤表面微结构成分的扫描电镜及能谱仪联用表征[J].分析测试学报,2008,27(11):1244-1247.
[18]Jiang R,Shan L,Wang X G.Study on the immobilization technology of bromelin with attapulgite modified by 3-aminopropyltriethoxysilane[J].Journal of Anhui Agricultural Sciences,2008,36(25):10739-10741.蒋荣,单良,王兴国.3-氨基丙基三乙氧基硅烷改性凹凸棒土固定化菠萝蛋白酶工艺研究[J].安徽农业科学,2008,36(25):10739-10741.