长裂苦苣菜甲醇提取物各极性成分的抗氧化活性研究
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摘要
目的:为探明长裂苦苣菜不同溶剂提取物活性功能成分及体外抗氧化性。方法:利用石油醚、乙酸乙酯、正丁醇、水为溶剂依次萃取长裂苦苣菜全草甲醇提取物,测定不同溶剂萃取物的总酚、总黄酮及单体酚含量,采用DPPH法、ABTS法及总还原力法评价其抗氧化活性,分析其酚类物质含量与抗氧化活性间的相关性。结果:HPLC分析检测到长裂苦苣菜中至少含有9种单体酚成分,其含量在不同溶剂萃取物中存在显著差异。乙酸乙酯萃取物的总酚、总黄酮含量最高,分别达到170.74 mg GA Eq./g和6.01 mg RU Eq./g,且其抗氧化活性最强。不同溶剂萃取物的抗氧化能力与萃取物的黄酮含量之间极显著相关(p<0.01)。结论:长裂苦苣菜中含丰富的酚类、黄酮类抗氧化活性成分,乙酸乙酯可作为长裂苦苣菜抗氧化活性物质富集溶剂,富集长裂苦苣菜中的咖啡酸、芦丁、荭草素、木犀草素等抗氧化活性功能成分。
Objective: In order to research the active components and antioxidant activities of different solvent extracts of Sonchus brachyotus. Result: different solvents such as petroleum ethyl,ethyl acetate,n- butanol and distilled water were used to extract S.brachyotus extracts obtained by methanol.The contents of total phenolics and flavonoids in different solvent extracts were analyzed and nine kinds of phenolics were detected by HPLC.Meanwhile,DPPH·,ABTS+· scavenging capacity and total reducing power were determined to compare antioxidant activity of different solvent extracts. The correlation between phenolics content of different solvent extracts and its antioxidant activities was analyzed.Result: There were nine kinds of phenolic components at least in Sonchus brachyotus detected by HPLC and the contents of them were different in different solvent extracts. Ethyl acetate extracts had the highest content of total phenolics( 170.74 mg GA Eq. / g) and flavonoids( 6.01 mg RU Eq. / g) and the strongest antioxidant activity. There was significant correlation between antioxidant activities of different solvent extracts and flavonoids content. Conculsion: The datum suggested that Sonchus brachyotus was rich in phenolics and flavonoids which had potent antioxidant activity. Ethyl acetate could be used as solvent-extraction to obtain the antioxidant in Sonchus brachyotus such as caffeic acid,rutin,orientin,luteolin et al.
Objective: In order to research the active components and antioxidant activities of different solvent extracts of Sonchus brachyotus. Result: different solvents such as petroleum ethyl,ethyl acetate,n- butanol and distilled water were used to extract S.brachyotus extracts obtained by methanol.The contents of total phenolics and flavonoids in different solvent extracts were analyzed and nine kinds of phenolics were detected by HPLC.Meanwhile,DPPH·,ABTS+· scavenging capacity and total reducing power were determined to compare antioxidant activity of different solvent extracts. The correlation between phenolics content of different solvent extracts and its antioxidant activities was analyzed.Result: There were nine kinds of phenolic components at least in Sonchus brachyotus detected by HPLC and the contents of them were different in different solvent extracts. Ethyl acetate extracts had the highest content of total phenolics( 170.74 mg GA Eq. / g) and flavonoids( 6.01 mg RU Eq. / g) and the strongest antioxidant activity. There was significant correlation between antioxidant activities of different solvent extracts and flavonoids content. Conculsion: The datum suggested that Sonchus brachyotus was rich in phenolics and flavonoids which had potent antioxidant activity. Ethyl acetate could be used as solvent-extraction to obtain the antioxidant in Sonchus brachyotus such as caffeic acid,rutin,orientin,luteolin et al.
引文
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[4]Khan R A.Evaluation of flavonoids and diverse antioxidant activities of Sonchus arvensis.[J].Chemistry Central Journal,2012,6(28):126-133.
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[6]Yin J,Heo S I,Jung M J,et al.Antioxidant activity of fractions from 70%methanolic extract of Sonchus oleraceus L.[J].Food Science&Biotechnology,2008,17(6):1299-1304.
[7]霍碧姗,秦民坚.苦苣菜属5种植物的黄酮类成分含量分析[J].植物资源与环境学报,2008,17(2):77-78.
[8]国旭丹,陕方,高锦明,等.苦荞麸乙醇粗提物各极性成分的抗氧化活性研究[J].中国食品学报,2011,11(6):20-26.
[9]Adom K K,Sorrells M E,Liu R H.Phytochemicals and antioxidant activity of milled fractions of different wheat varieties[J].Journal of Agricultural and Food Chemistry,2005,53(6):2297-2306.
[10]Inglett G E,Rose D J,Chen D,et al.Phenolic content and antioxidant activity of extracts from whole buckwheat(Fagopyrum esculentum Mench)with or without microwave irradiation[J].Food Chemistry,2010,119(3):1216-1219.
[11]Gao Q H,Wu C S,Yu J G.Textural characteristic,antioxidant activity,sugar,organic acid,and phenolic profiles of10 promising jujube(Ziziphus jujuba Mill.)selections[J].Journal of Food Science,2012,77(11):C1218-C1225.
[12]阎林茂,郭宇龙,张延龙,等.百合花瓣酚类物质及其抗氧化活性的分析[J].食品科学,2013,34(7):51-55.
[2]中药研究所.全国中草药名鉴[M].北京:人民卫生出版社,1996:797.
[3]夏正祥,唐中艳,梁敬钰,等.苦苣菜属植物化学成分与药理作用研究进展[J].中国实验方剂学杂志,2012,18(14):300-306.
[4]Khan R A.Evaluation of flavonoids and diverse antioxidant activities of Sonchus arvensis.[J].Chemistry Central Journal,2012,6(28):126-133.
[5]Khan R A,Khan M R,Sahreen S,et al.Evaluation of phenolic contents and antioxidant activity of various solvent extracts of Sonchus asper(L.)Hill.[J].Chemistry Central Journal,2012,6(1):48-55.
[6]Yin J,Heo S I,Jung M J,et al.Antioxidant activity of fractions from 70%methanolic extract of Sonchus oleraceus L.[J].Food Science&Biotechnology,2008,17(6):1299-1304.
[7]霍碧姗,秦民坚.苦苣菜属5种植物的黄酮类成分含量分析[J].植物资源与环境学报,2008,17(2):77-78.
[8]国旭丹,陕方,高锦明,等.苦荞麸乙醇粗提物各极性成分的抗氧化活性研究[J].中国食品学报,2011,11(6):20-26.
[9]Adom K K,Sorrells M E,Liu R H.Phytochemicals and antioxidant activity of milled fractions of different wheat varieties[J].Journal of Agricultural and Food Chemistry,2005,53(6):2297-2306.
[10]Inglett G E,Rose D J,Chen D,et al.Phenolic content and antioxidant activity of extracts from whole buckwheat(Fagopyrum esculentum Mench)with or without microwave irradiation[J].Food Chemistry,2010,119(3):1216-1219.
[11]Gao Q H,Wu C S,Yu J G.Textural characteristic,antioxidant activity,sugar,organic acid,and phenolic profiles of10 promising jujube(Ziziphus jujuba Mill.)selections[J].Journal of Food Science,2012,77(11):C1218-C1225.
[12]阎林茂,郭宇龙,张延龙,等.百合花瓣酚类物质及其抗氧化活性的分析[J].食品科学,2013,34(7):51-55.