明日叶不同极性溶剂萃取物的活性研究
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摘要
为比较明日叶不同极性部位的活性,将明日叶粗提取物过HPD-600大孔树脂,收集50%乙醇洗脱物。采用不同极性有机溶剂对50%洗脱物进行液—液萃取,将50%洗脱物分为乙酸乙酯相、正丁醇相、水相三个不同极性溶剂萃取物,测定不同极性溶剂萃取物的总黄酮和总多酚含量,研究各萃取物清除DPPH自由基、ABTS自由基能力和总还原能力以及对α-葡萄糖苷酶的抑制活性。结果表明:乙酸乙酯相的总黄酮含量以及总多酚含量高于其他溶剂相,且体外抗氧化能力和对α-葡萄糖苷酶的抑制活性最强。明日叶不同极性溶剂萃取物的抗氧化活性和对α-葡萄糖苷酶的抑制率随着浓度的增加而增加。
In order to compare the activities of different extracts using several polar fractions of extracts from AngelicaKeiskei,the crude extracts were adsorbed by HPD-600 macroporous resin,and 50%ethanol eluents were collected as products.The products were separated into three different polar solvents for extracting,i.e.,ethyl acetate fraction,n-butanol fraction and aqueous fraction,and extracted by organic solvents of different polarities based on liquids-liquids extracting method.Moreover,the contents of total phenolic and flavonoid in different extracts were determined,and then the activities on scavenging DPPH,ABTS radical,ferric-reducing power and inhibiting toα-glucosidase were also studied.The results showed that the ethyl acetate-soluble fraction had high contents of total phenolic and flavonoid with the strongest antioxidant andα-glucosidase inhibitory activities.The antioxidant activity and inhibitory rate ofα-glucosidase of the extracts from A.Keiskei increased according with the their concentration increasing.
In order to compare the activities of different extracts using several polar fractions of extracts from AngelicaKeiskei,the crude extracts were adsorbed by HPD-600 macroporous resin,and 50%ethanol eluents were collected as products.The products were separated into three different polar solvents for extracting,i.e.,ethyl acetate fraction,n-butanol fraction and aqueous fraction,and extracted by organic solvents of different polarities based on liquids-liquids extracting method.Moreover,the contents of total phenolic and flavonoid in different extracts were determined,and then the activities on scavenging DPPH,ABTS radical,ferric-reducing power and inhibiting toα-glucosidase were also studied.The results showed that the ethyl acetate-soluble fraction had high contents of total phenolic and flavonoid with the strongest antioxidant andα-glucosidase inhibitory activities.The antioxidant activity and inhibitory rate ofα-glucosidase of the extracts from A.Keiskei increased according with the their concentration increasing.
引文
[1]吴敬章,王磊,张菊平.明日叶的价值及其开发利用[J].中国野生植物资源,2015,34(5):60-61.
[2]YANG Meng,JING Yi-zhong,HE Sun.Effect of Angelica keiskei chalcone on the expression of apoptosis-regulating proteins of mice hepatocarcinoma cells[J].Chinese-German Journal of Clinical Oncology,2011,10(6):325-327.
[3]ENOKI T,OHNOGI H,NAGAMINE K,et al.Antidiabetic activities of chalcones isolated from a Japanese Herb,Angelica keiskei[J].Journal of agricultural and food chemistry,2007,55(15):6 013-6 017
[4]刘畅,王正武,吴金鸿.药食兼用植物明日叶的研究进展及应用[J].食品与药品,2015,15(3):205-209.
[5]HU Yao-ming,CHU Dong-hai.Study on Ultrasonic extraction of total flavonoids from Angelica keiskei Koidzumi[J].Agricultural Biotechnology,2016,5(3):6-8.
[6]郭晓青,吴金鸿,周焱富,等.明日叶叶水溶性总黄酮提纯工艺[J].食品科学,2012,33(22):27-32.
[7]刘程程,韦万丽,廖莉玲.6种黔产清热类中草药主要抗氧化成分的含量[J].贵州农业科学,2012,40(2):41-43
[8]崔紫姣,张彩云,刘彬彬,等.Folin-Ciocalteu比色法测定甜茶总多酚含量[J].贵州农业科学,2014,42(3):158-160.
[9]郄佩娟,段旭昌,王敏,等.长裂苦苣菜甲醇提取物各极性成分的抗氧化活性研究[J].食品工业科技,2016,37(16):146-150.
[10]曹清明,邬靖宇,钟海雁,等.油茶叶中黄酮的超声辅助提取及其抗氧化活性研究[J].食品与机械,2015,31(3):162-166.
[11]欧阳凯,何先元,陈飞,等.四齿四棱草总皂苷提取工艺优化及抗氧化性研究[J].食品与机械,2016,32(7):141-145.
[12]李晨悦,李冬利,孙玉坤,等.簕菜提取物及其化学成分对α-葡萄糖苷酶的抑制作用[J].食品工业,2016,37(4):164-166.
[13]李培.山药多糖对α-葡萄糖苷酶的抑制作用动力学研究[J].食品与机械,2016,32(7):14-17.
[14]尹红力,赵鑫,佟丽丽,等.黑木耳多糖体外和体内降血糖功能[J].食品科学,2015,36(21):221-226.
[2]YANG Meng,JING Yi-zhong,HE Sun.Effect of Angelica keiskei chalcone on the expression of apoptosis-regulating proteins of mice hepatocarcinoma cells[J].Chinese-German Journal of Clinical Oncology,2011,10(6):325-327.
[3]ENOKI T,OHNOGI H,NAGAMINE K,et al.Antidiabetic activities of chalcones isolated from a Japanese Herb,Angelica keiskei[J].Journal of agricultural and food chemistry,2007,55(15):6 013-6 017
[4]刘畅,王正武,吴金鸿.药食兼用植物明日叶的研究进展及应用[J].食品与药品,2015,15(3):205-209.
[5]HU Yao-ming,CHU Dong-hai.Study on Ultrasonic extraction of total flavonoids from Angelica keiskei Koidzumi[J].Agricultural Biotechnology,2016,5(3):6-8.
[6]郭晓青,吴金鸿,周焱富,等.明日叶叶水溶性总黄酮提纯工艺[J].食品科学,2012,33(22):27-32.
[7]刘程程,韦万丽,廖莉玲.6种黔产清热类中草药主要抗氧化成分的含量[J].贵州农业科学,2012,40(2):41-43
[8]崔紫姣,张彩云,刘彬彬,等.Folin-Ciocalteu比色法测定甜茶总多酚含量[J].贵州农业科学,2014,42(3):158-160.
[9]郄佩娟,段旭昌,王敏,等.长裂苦苣菜甲醇提取物各极性成分的抗氧化活性研究[J].食品工业科技,2016,37(16):146-150.
[10]曹清明,邬靖宇,钟海雁,等.油茶叶中黄酮的超声辅助提取及其抗氧化活性研究[J].食品与机械,2015,31(3):162-166.
[11]欧阳凯,何先元,陈飞,等.四齿四棱草总皂苷提取工艺优化及抗氧化性研究[J].食品与机械,2016,32(7):141-145.
[12]李晨悦,李冬利,孙玉坤,等.簕菜提取物及其化学成分对α-葡萄糖苷酶的抑制作用[J].食品工业,2016,37(4):164-166.
[13]李培.山药多糖对α-葡萄糖苷酶的抑制作用动力学研究[J].食品与机械,2016,32(7):14-17.
[14]尹红力,赵鑫,佟丽丽,等.黑木耳多糖体外和体内降血糖功能[J].食品科学,2015,36(21):221-226.