3种药食同源植物总黄酮提取条件及其抑制禾谷镰刀菌的作用
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摘要
采用超声波破碎法提取3种药食同源植物(马齿苋、长裂苦苣菜、蒲公英)中的总黄酮,考察乙醇浓度、料液比(mg/m L)、p H、超声时间、超声功率、超声温度对3种植物总黄酮提取量的影响,确定3种植物总黄酮适宜提取条件。实验结果表明,在3种植物总黄酮适宜提取条件下,马齿苋、长裂苦苣菜、蒲公英总黄酮提取量分别达到3.82、28.57、9.17 mg/g。另外,分析3种植物总黄酮提取物对禾谷镰刀菌的抑制效果。结果显示,当3种植物总黄酮提取物质量浓度为50 mg/m L和100 mg/m L时,提取物对禾谷镰刀菌的抑制作用为:马齿苋提取物>蒲公英提取物>长裂苦苣菜提取物;当低于50 mg/m L时,3种提取物对禾谷镰刀菌的抑制作用为:蒲公英提取物>马齿苋提取物>长裂苦苣菜提取物。
This experiment used the method of ultrasonic disintegration to extract the total flavonoids from three kinds of medicinal and edible plant(Portulaca oleracea L., Sonchus brachyotus DC., Taraxacum mongolicum Hand. Mazz.). Six conditions including ethanol proportion, solid-liquid ratio, p H, ultrasonic time, ultrasonic power, ultrasonic temperature were investigated to determine the optimum conditions for extraction of total flavonoids from three plants. The results showed that: under the appropriate extraction conditions, the yield of total flavonoids in Portulaca oleracea L., Sonchus brachyotus DC. and Taraxacum mongolicum Hand. Mazz. was 3.82 mg/g, 28.57 mg/g and 9.17 mg/g respectively. Moreover, the inhibition effects of three kinds of plant extracts on Fusarium graminearum were conducted. The results were as follows: when the concentration of the three plant extracts was 50 mg/m L and 100 mg/m L, the inhibitory effect of the extract on Fusarium graminearum was: Portulaca oleracea L.>Taraxacum mongolicum Hand.>Mazz. Sonchus brachyotus DC., however, when the concentration was below 50 mg/m L, the inhibitory effect of the extract on Fusarium graminearum was: Taraxacum mongolicum Hand.>Portulaca oleracea L.>Mazz. Sonchus brachyotus DC.
This experiment used the method of ultrasonic disintegration to extract the total flavonoids from three kinds of medicinal and edible plant(Portulaca oleracea L., Sonchus brachyotus DC., Taraxacum mongolicum Hand. Mazz.). Six conditions including ethanol proportion, solid-liquid ratio, p H, ultrasonic time, ultrasonic power, ultrasonic temperature were investigated to determine the optimum conditions for extraction of total flavonoids from three plants. The results showed that: under the appropriate extraction conditions, the yield of total flavonoids in Portulaca oleracea L., Sonchus brachyotus DC. and Taraxacum mongolicum Hand. Mazz. was 3.82 mg/g, 28.57 mg/g and 9.17 mg/g respectively. Moreover, the inhibition effects of three kinds of plant extracts on Fusarium graminearum were conducted. The results were as follows: when the concentration of the three plant extracts was 50 mg/m L and 100 mg/m L, the inhibitory effect of the extract on Fusarium graminearum was: Portulaca oleracea L.>Taraxacum mongolicum Hand.>Mazz. Sonchus brachyotus DC., however, when the concentration was below 50 mg/m L, the inhibitory effect of the extract on Fusarium graminearum was: Taraxacum mongolicum Hand.>Portulaca oleracea L.>Mazz. Sonchus brachyotus DC.
引文
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[13]王秋红,刘畅,方波.花生壳黄酮微波碱法提取工艺研究[J].食品科技,2010,35(01):207-210
[14]周邦靖.常用中药的抗菌作用及其测定方法[M].重庆:科学技术出版社重庆分社,1987:5-38
[15]李菊花,赵孟辉,陈波.不同乙醇浓度提取对总黄酮测定的影响[J].浙江预防医学,1999,(10):63-64
[16]曹雁平,刘佐才.植物成分超声浸取研究现状[J].化工进展,2005,(11):58-61
[17]PERLA V,HOLM DG,JAYANTY S S.Effects of cooking methods on Polyphenols,pigments and antioxidant activity in potato tubers[J].LWT-Food Science and technology,2012,45(2):161-171
[2]房祥军,邹晓庭.饲料霉菌毒素污染的危害及其防治[J].中国饲料,2006,(11):34-37
[3]杨信,范小平,杨绮纹,等.自然污染谷物中镰刀菌毒素对家禽的危害研究进展[J].饲料研究,2014,(05):56-59
[4]GIRISH C K,SMITH T K,BOERMANS H J,et al.Effects of feeding blends of grains naturally contaminated with Fusarium mycotoxins on performance,hematology,metabolism,and immunocompetence of turkeys[J].Poultry Science,2008,87(3):421-432
[5]彭杰,吴晓鹏,黄惠琴,等.镰刀菌毒素研究进展[J].中国农学通报,2009,25(02):25-27
[6]刘瑞丽,郑云峰.饲用防霉剂的研究进展[J].中国饲料添加剂,2012,(5):11-12
[7]春桃,曾一民,刘慎.植物源天然防霉剂的研究与应用现状[J].湖南饲料,2006,(03):34-35
[8]王海威,应光耀,杨志欣,等.基于中草药的天然防霉剂的研究进展与剂型开发[J].中国中药杂志,2017,42(07):1251-1257
[9]刘一杰,薛永常.植物黄酮类化合物的研究进展[J].中国生物工程杂志,2016,36(09):81-86
[10]顾采琴,谢琳琳,张织芬,等.香蕉皮黄酮的分离纯化及抑菌活性研究[J].食品科学,2013,34(16):98-102
[11]陈国妮,孙飞龙,闫亚茹,等.马齿苋黄酮类化合物抑菌机制的研究[J].化学与生物工程,2015,32(10):34-37
[12]周立刚,张颖君,蔡艳,等.黄酮和甾体类化合物的抗真菌活性[J].天然产物研究与开发,1997,(03):24-29
[13]王秋红,刘畅,方波.花生壳黄酮微波碱法提取工艺研究[J].食品科技,2010,35(01):207-210
[14]周邦靖.常用中药的抗菌作用及其测定方法[M].重庆:科学技术出版社重庆分社,1987:5-38
[15]李菊花,赵孟辉,陈波.不同乙醇浓度提取对总黄酮测定的影响[J].浙江预防医学,1999,(10):63-64
[16]曹雁平,刘佐才.植物成分超声浸取研究现状[J].化工进展,2005,(11):58-61
[17]PERLA V,HOLM DG,JAYANTY S S.Effects of cooking methods on Polyphenols,pigments and antioxidant activity in potato tubers[J].LWT-Food Science and technology,2012,45(2):161-171