气质联用测定苦苣菜属三种植物的挥发油成分
|
摘要
目的对苣荬菜、长裂苦苣菜、花叶滇苦菜中的挥发油成分进行分析与比较。方法用水蒸气蒸馏法分别提取苣荬菜、长裂苦苣菜、花叶滇苦菜的挥发油,采用气相色谱-质谱联用法,鉴定其挥发油的化学成分。结果苣荬菜鉴定出35种成分,长裂苦苣菜鉴定出55种成分,花叶滇苦菜鉴定出49种成分。结论苣荬菜的主要成分为6,10,14-三甲基-十五烷-2-酮(26.46%)、植醇(9.67%),长裂苦苣菜的主要成分为正十六烷酸(8.01%)、4-(2,6,6-三甲基-1-环己烯-1-基)-3-丁烯-2-酮(7.09%),花叶滇苦菜的主要成分为正十六烷酸(12.87%)、6,10,14-三甲基-十五烷-2-酮(12%)。三者共同的成分有6,10,14-三甲基-十五烷-2-酮、1,1,5-三甲基-1,2-二氢萘、(E)-1-(2,6,6-三甲基-1,3-环己二烯-1-基)-2-丁烯-1-酮等11种。
Objective: To analyze and compare the volatile oil components in endive,sonchus brachyotus and sonchus asper. Method: volatile oils of endive,sonchus brachyotus and sonchus asper were extracted by steam distillation and their chemical constituents were identified by GC-MS. Result: 35 components were identified from endive,55 components were identified from sonchus brachyotus,49 components were identified from sonchus asper. Conclusion: The main components of endive are 6,10,14-trimethyl-pentadecan-2-ketone(26. 46%) and phytol(9. 67%). The main components of sonchus brachyotus are n-hexadecanoic acid(8. 01%) and 4-(2,6,6-trimethyl-1-cyclohexene-1-yl)-3-butene-2-ketone(7. 09%). The main components of sonchus asper are n-hexadecanoic acid(12. 87%) and 6,10,14-trimethyl-pentadecan-2-ketone(12%). The common components of the three are 6,10,14-trimethyl-pentadecan-2-ketone,1,1,5-trimethyl-1,2-dihydronaphtha,(E)-1-(2,6,6-trimethyl-1-cyclohexene-1-yl)-2-butene-1-ketone and other 8 species.
Objective: To analyze and compare the volatile oil components in endive,sonchus brachyotus and sonchus asper. Method: volatile oils of endive,sonchus brachyotus and sonchus asper were extracted by steam distillation and their chemical constituents were identified by GC-MS. Result: 35 components were identified from endive,55 components were identified from sonchus brachyotus,49 components were identified from sonchus asper. Conclusion: The main components of endive are 6,10,14-trimethyl-pentadecan-2-ketone(26. 46%) and phytol(9. 67%). The main components of sonchus brachyotus are n-hexadecanoic acid(8. 01%) and 4-(2,6,6-trimethyl-1-cyclohexene-1-yl)-3-butene-2-ketone(7. 09%). The main components of sonchus asper are n-hexadecanoic acid(12. 87%) and 6,10,14-trimethyl-pentadecan-2-ketone(12%). The common components of the three are 6,10,14-trimethyl-pentadecan-2-ketone,1,1,5-trimethyl-1,2-dihydronaphtha,(E)-1-(2,6,6-trimethyl-1-cyclohexene-1-yl)-2-butene-1-ketone and other 8 species.
引文
[1]姚玉霞.苦苣菜、小根蒜营养成分分析[J].营养学报,2003,25(2):173-174.
[2]中国中医研究院中药研究所.全国中草药名鉴[M].北京:人民卫生出版社,1996:797.
[3]刘磊,李静,陈燕平.苦苣菜和苣荬菜茎叶中脂肪酸含量分析[J].吉林大学学报(医学版),2002,28(6):606-607.
[4]霍建中.微波消解电感耦合等离子体原子发射光谱法测定苦苣菜根、茎、叶中常量及微量元素[J].分析化学,2003,31(5):640-624.
[5]姜祎,徐虹,秦天福,等.GC-MS分析流苏虾脊兰叶挥发性化学成分[J].现代中医药,2015,35(4):56-58.
[6]从浦珠.质谱学在天然有机化学中的应用[M].北京:科学出版社,1987.
[7]Hellor SR.EPA/NIH Mass spectral Data Base[M].Washington US:Government Printing Office,1980:1915
[8]MasadaY.AnalysisofEssentialOilby)GasChromatogroghyandMassSpectrometry[M].New York:Wiley Inc,1993:125-133.
[9]李长恭,渠桂荣,牛红英,等.苣荬菜花的挥发油成分分析[J].河南师范大学学报(自然科学版),2005,33(2):128-132.
[10]许欢,王菲,张丽,等.高效液相色谱法测定二色补血草中杨梅苷、圣草酚、木犀草素、斛皮素的含量[J].陕西中医药大学学报,2017,40(3):60-68.
[11]徐朋,竺锡武,陈集双,等.长裂苦苣菜挥发油成分的GC/MS分析[J].科技通报,2010,26(3):374-379.
[12]SCHAFFER S,SCHMITT-SCHILLIG S,MüLLER W E,et al.Antioxidant Properties Of Mediterranean Food Plant Extracts:Geographical differences[J].Journal Of Physiology And Pharmacology,2005,56(Suppl 1):115-124.
[13]王慎敏.日用化学品[M].北京:化学工业出版社,2005:524.
[14]朱荫,杨停,施江,等.西湖龙井茶香气成分的全二维气相色谱-飞行时间质谱分析[J].中国农业科学,2015,48(20):4120-4146.
[15]高向阳,张娜,史可夫.不同生长期续断菊中氨基酸柱前衍生RP-HPLC同时测定及营养特性比较[J].广东农业科学,2013,12(3):111-114.
[2]中国中医研究院中药研究所.全国中草药名鉴[M].北京:人民卫生出版社,1996:797.
[3]刘磊,李静,陈燕平.苦苣菜和苣荬菜茎叶中脂肪酸含量分析[J].吉林大学学报(医学版),2002,28(6):606-607.
[4]霍建中.微波消解电感耦合等离子体原子发射光谱法测定苦苣菜根、茎、叶中常量及微量元素[J].分析化学,2003,31(5):640-624.
[5]姜祎,徐虹,秦天福,等.GC-MS分析流苏虾脊兰叶挥发性化学成分[J].现代中医药,2015,35(4):56-58.
[6]从浦珠.质谱学在天然有机化学中的应用[M].北京:科学出版社,1987.
[7]Hellor SR.EPA/NIH Mass spectral Data Base[M].Washington US:Government Printing Office,1980:1915
[8]MasadaY.AnalysisofEssentialOilby)GasChromatogroghyandMassSpectrometry[M].New York:Wiley Inc,1993:125-133.
[9]李长恭,渠桂荣,牛红英,等.苣荬菜花的挥发油成分分析[J].河南师范大学学报(自然科学版),2005,33(2):128-132.
[10]许欢,王菲,张丽,等.高效液相色谱法测定二色补血草中杨梅苷、圣草酚、木犀草素、斛皮素的含量[J].陕西中医药大学学报,2017,40(3):60-68.
[11]徐朋,竺锡武,陈集双,等.长裂苦苣菜挥发油成分的GC/MS分析[J].科技通报,2010,26(3):374-379.
[12]SCHAFFER S,SCHMITT-SCHILLIG S,MüLLER W E,et al.Antioxidant Properties Of Mediterranean Food Plant Extracts:Geographical differences[J].Journal Of Physiology And Pharmacology,2005,56(Suppl 1):115-124.
[13]王慎敏.日用化学品[M].北京:化学工业出版社,2005:524.
[14]朱荫,杨停,施江,等.西湖龙井茶香气成分的全二维气相色谱-飞行时间质谱分析[J].中国农业科学,2015,48(20):4120-4146.
[15]高向阳,张娜,史可夫.不同生长期续断菊中氨基酸柱前衍生RP-HPLC同时测定及营养特性比较[J].广东农业科学,2013,12(3):111-114.