响应面法优化长裂苦苣菜总生物碱提取工艺
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摘要
为了优化长裂苦苣菜中总生物碱的提取工艺。采用单因素实验分析料液比、超声时间、超声温度、超声功率、pH、乙醇浓度对总生物碱提取率的影响。选取4因素3水平,采用响应面法优化总生物碱提取工艺,并分析各个因素的显著性和交互作用。结果表明:确定最佳提取条件为:料液比1∶30 g/m L,超声时间30 min,超声温度55℃,超声功率700 W,pH为5,乙醇浓度75%,总生物碱提取率为20.30%。结论:实验结果表明二次多项式建立的数学模型具有良好的预测性,响应面法筛选得到的总生物碱提取工艺可行,稳定,并且重现性好。
To optimize the best technique for extracting the total alkaloids from Sonchus brachyotus DC.. The single factor experiment was utilized to analyze the impact of the liquid-solid ratio,ultrasonic time,ultrasonic temperature,ultrasonic power,pH,ethanol concentration on the yield of total alkaloids. The method of response surface analysis with four factors and three levels was adopted to optimize the extraction process of total alkaloids,and the importance and the interactions of disparate factors were analyzed. The optimum technological circumstances were resolved as follows: solid-liquid ratio 1 ∶ 30 g/m L,ultrasonic time 30 min,ultrasonic temperature 55 ℃,ultrasonic power 700 W,pH 5,ethanol concentration 75%,and the total alkaloids yield was 20.30%.Conclusion: the experimental consequences demonstrated that the mathematical model of quadratic polynomial had favorable predictability,and the total alkaloids extraction process was steady,feasible and repeatable.
To optimize the best technique for extracting the total alkaloids from Sonchus brachyotus DC.. The single factor experiment was utilized to analyze the impact of the liquid-solid ratio,ultrasonic time,ultrasonic temperature,ultrasonic power,pH,ethanol concentration on the yield of total alkaloids. The method of response surface analysis with four factors and three levels was adopted to optimize the extraction process of total alkaloids,and the importance and the interactions of disparate factors were analyzed. The optimum technological circumstances were resolved as follows: solid-liquid ratio 1 ∶ 30 g/m L,ultrasonic time 30 min,ultrasonic temperature 55 ℃,ultrasonic power 700 W,pH 5,ethanol concentration 75%,and the total alkaloids yield was 20.30%.Conclusion: the experimental consequences demonstrated that the mathematical model of quadratic polynomial had favorable predictability,and the total alkaloids extraction process was steady,feasible and repeatable.
引文
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[2]龙凤来,陈美红.苦苣菜属植物的药学概况[J].产业与科技论坛,2017,16(8):80-82.
[3]李英姬.苣荬菜的化学成分与临床应用[J].中国实用医药,2009,4(14):155-156.
[4]刘磊,李静,陈燕萍.苣荬菜、苦荬菜、苣荬菜茎叶中脂肪酸含量分析[J].吉林大学学报:医学版,2002,28(6):606-607.
[5]徐朋,竺锡武,陈集双.长裂苦苣菜挥发油成分的GC/MS分析[J].科技通报,2010,26(3):374-379.
[6]李记争,杨光,马琳,等.苦苣菜水提物对实验性糖尿病小鼠降血糖作用的研究[J].时珍国医国药,2011,22(2):419-421.
[7]Xia D Z,Yu X F,Zhu Z Y,et al.Antioxidant and antibacterial activity of six edible wild plants(Sonchus spp.)in China[J].Natural Product Research,2011,25(20):1893-1901.
[8]李杨,左国营.生物碱类化合物抗菌活性研究进展[J].中草药,2010,41(6):1006-1014.
[9]柏云娇,于淼,赵思伟,等.生物碱的药理作用及机制研究[J].哈尔滨商业大学学报:自然科学版,2013,29(1):8-11.
[10]赵娜,高峰,刘彬,等.苄基四氢异喹啉类生物碱的药理作用及其研究进展[J].药学实践杂志,2015,33(4):313-315.
[11]于蕾,张连芳,东方,等.苦豆子中主要生物碱药理作用的研究进展[J].北京联合大学学报,2015,29(2):61-66.
[12]高齐,段欢,赵盼登,等.生物碱提取方法的研究进展[J].杭州化工,2014,(2):12-14.
[13]何军,祝林,奉建芳.附子总生物碱含量测定方法比较[J].现代中药研究与实践,2003,17(6):20-22.
[14]Hu J,Zhang L,Min Y J,et al. Study of the extracting technology of alkaloid from Lycoris radiate[J].Forest By-Product Speciality in China,2007,89(4):8-12.
[15]Song Z Q,Ao M H.Research progress of alkaloids in Uncaria[J].Medicinal Plant,2014,5(5):56-58.
[16]马涛,张秋龙,丁晨曦,等.响应面法优化假北紫堇总生物碱提取工艺[J].中药材,2015,38(8):1736-1740.
[17]吴三桥,梁引库.巨大型蒲公英生物碱的超声提取工艺[J].贵州农业科学,2013,41(5):130-132.