长裂苦苣菜萃取物对胰岛素抵抗HepG2细胞糖代谢的影响
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摘要
使用高浓度胰岛素来诱导HepG2细胞,使之出现葡萄糖代谢的异常,以此建造胰岛素抵抗HepG2/IR细胞模型,利用葡萄糖测定试剂盒检测长裂苦苣菜石油醚、乙酸乙酯、正丁醇萃取部位组葡萄糖消耗量,研究长裂苦苣菜不同萃取部位对胰岛素抵抗HepG2细胞模型糖代谢的影响.结果显示,10-6mol/L浓度的胰岛素诱导处理HepG2细胞24h是产生胰岛素抵抗的最佳条件;与HepG2/IR模型组相比,各萃取部位均可以改善HepG2/IR细胞的葡萄糖消耗情况,以乙酸乙酯部位效果最佳.结果表明长裂苦苣菜提取物有一定的改善胰岛素抵抗的作用.
The purpose of the present paper is to study the different extraction parts from Sonchus brachyotus D C. on glucose metabolism in insulin resistant Hep G2 cells. The model of insulin resistance in Hep G2 cells induced high concentrations of insulin. Glucose consumption is tested on petroleum ether, ethyl acetate and n-butanol extraction. Concentration of 10-6 mol/L insulin is found to be the best condition for Hep G2 cells 24 h to generate insulin resistance. Compared with model group Hepg2/IR, the Sonchus brachyotus D C.'s extraction parts can reduce the Hep G2 / IR glucose consumption. It works especially well on the ethyl acetate extract. The results show that Sonchus brachyotus D C.'s extraction parts can improve the effect of insulin resistance.
The purpose of the present paper is to study the different extraction parts from Sonchus brachyotus D C. on glucose metabolism in insulin resistant Hep G2 cells. The model of insulin resistance in Hep G2 cells induced high concentrations of insulin. Glucose consumption is tested on petroleum ether, ethyl acetate and n-butanol extraction. Concentration of 10-6 mol/L insulin is found to be the best condition for Hep G2 cells 24 h to generate insulin resistance. Compared with model group Hepg2/IR, the Sonchus brachyotus D C.'s extraction parts can reduce the Hep G2 / IR glucose consumption. It works especially well on the ethyl acetate extract. The results show that Sonchus brachyotus D C.'s extraction parts can improve the effect of insulin resistance.
引文
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[2]Ma J Z,Yang L X,Shen X L,et al.Effects of Traditional Chinese Medicinal Plants on Anti-insulin Resistance Bioactivity of DXMS-Induced Insulin Resistant Hep G2Cells[J].Nat Prod Bioprospect,2014,4(4):197-206.
[3]俞发荣,连秀珍,谢明仁,等.胰岛素抵抗细胞与大鼠模型的建立及其应用[J].中国实验动物学报,2013(06):74-78.
[4]李英姬.苣荬菜的化学成分与临床应用[J].中国实用医药,2009,4(14):155-156.
[5]刘磊,李静,陈燕萍.苣荬菜、苦荬菜、苣荬菜茎叶中脂肪酸含量分析[J].吉林大学学报(医学版),2002,28(6):606-607.
[6]徐朋,竺锡武,陈集双.长裂苦苣菜挥发油成分的GC/MS分析[J].科技通报,2010,26(3):374-379.
[7]李记争,杨光,马琳,等.苦苣菜水提物对实验性糖尿病小鼠降血糖作用的研究[J].时珍国医国药,2011,22(2):419-421.
[8]XIA D Z,YU X F,ZHU Z Y,et al.Antioxidant and antibacterial activity of six edible wild plants(Sonchus spp.)in China[J].Nat Prod Res,2011,25(20):1893-1901.
[9]Samuel V T,Shulman G I.Mechanisms for insulin resistance:common threads and missing link[J].Cell,2012,148(5):852-871.