高效液相色谱法测定脑栓康复浓缩丸三七中人参皂苷R_(g1)的含量

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英文篇名：Determination of Ginsenoside-R_(g1) in Naoshuankangfu concentrated pills by HPLC 作者：刘宏 ; 王兰英 ; 任建国 ; 赵立军 英文作者：Liu Hong;Wang Lanying;Ren Jianguo;Zhao Lijun;Pharmacology School,Inner Mongolian Medical University;Inner Mongolia Otaqi Mongolia Medicine Co.,Ltd.; 关键词：高效液相色谱 ; 人参皂苷Rg1 ; 脑栓康复浓缩丸 ; 含量测定 英文关键词：HPLC;;Ginsenoside-Rg1;;Naoshuankangfu;;Quantitative determination 中文刊名：BFYX 英文刊名：Journal of North Pharmacy 机构：内蒙古医科大学药学院;内蒙古奥特奇蒙药股份有限公司; 出版日期：2014-01-01 出版单位：北方药学 年：2014 期：v.11 语种：中文; 页：BFYX201401004 页数：2 CN：01 ISSN：15-1333/R 分类号：14-15

摘要

目的:建立脑栓康复浓缩丸中人参皂苷R g1的含量测定方法。方法:采用反相高效液相色谱(RP-HPLC)法,色谱柱Diamosil C18柱(4.6mm×250mm,5μm),流动相为乙腈-0.1%磷酸溶液(20∶80);检测波长为203nm,流速为1ml/min,进样量为20μl。结果:人参皂苷Rg1的线性范围1.77~17.70μg(r=0.9998),平均加样回收率(N=6)100.30%,RSD 1.35%。结论:本法准确、简便,重复性好,可作为脑栓康复浓缩丸的质量控制方法。
        Objective:To establish a HPLC method for determining the content of Ginsenoside-R g1 in Naoshuankangfu concentrated pills.Methods:A chromatographic column of Diamosil C 18(4.6mm×250mm,5μm)was used,the mobile phase was acetonitrile-0.1% Phosphoric acid(20∶80).The flow velocity was 1ml/min,detection wave length was 203nm.Results :The linear range of Ginsenoside- Rg1 was 1.77~17.70μg(r=0.9998),average recovery(n=6)100.30%(RSD 1.35%).Conclusion:The method Was accurate and simple with a higher repeatability,which Can be used for the quality control of Naoshuankangfu concentrated pills.

引文

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