摘要
目的:建立脑栓康复浓缩丸中人参皂苷R g1的含量测定方法。方法:采用反相高效液相色谱(RP-HPLC)法,色谱柱Diamosil C18柱(4.6mm×250mm,5μm),流动相为乙腈-0.1%磷酸溶液(20∶80);检测波长为203nm,流速为1ml/min,进样量为20μl。结果:人参皂苷Rg1的线性范围1.77~17.70μg(r=0.9998),平均加样回收率(N=6)100.30%,RSD 1.35%。结论:本法准确、简便,重复性好,可作为脑栓康复浓缩丸的质量控制方法。
Objective:To establish a HPLC method for determining the content of Ginsenoside-R g1 in Naoshuankangfu concentrated pills.Methods:A chromatographic column of Diamosil C 18(4.6mm×250mm,5μm)was used,the mobile phase was acetonitrile-0.1% Phosphoric acid(20∶80).The flow velocity was 1ml/min,detection wave length was 203nm.Results :The linear range of Ginsenoside- Rg1 was 1.77~17.70μg(r=0.9998),average recovery(n=6)100.30%(RSD 1.35%).Conclusion:The method Was accurate and simple with a higher repeatability,which Can be used for the quality control of Naoshuankangfu concentrated pills.
引文
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