12种氯苯类化合物纯度定值色谱方法研究
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摘要
对用于溶液标准物质制备的12种氯苯类纯品原料进行了高效液相色谱法(HPLC-DAD)和气相色谱法(GC-FID)两种纯度定值方法的研究。在优化的色谱条件下,甲醇基体混合溶液中的12个目标物组分包含一氯代苯、1,2-二氯苯、1,4-二氯苯、1,3-二氯苯、1,2,3-三氯苯、1,2,4-三氯苯、1,3,5-三氯苯、1,2,3,4-四氯苯、1,2,4,5-四氯苯、1,2,3,5-四氯苯、五氯苯、六氯苯均能良好分离。避免了氯苯类原料中存在的同分异构体及同系物杂质与主体成分没有良好分离而导致的对纯度定值结果的影响。
The research of purity chromatography measurement method( HPLC-DAD and GC-FID) for 12 kinds of chlorobenzenes raw materials which were used to prepare chlorobenzenes solution reference material was introduced. It contains monochlorobenzene,1,2-dichlorobenzene,1,4-dichlorobenzene,1,3-dichlorobenzene 1,2,3-trichlorobenzene,1,2,4-trichlorobenzene,1,3,5-trichlorobenzene,1,2,3,4-tetrachlorobenzene,1,2,4,5-tetrachlorobenzene,1,2,3,5-tetrachlorobenzene,pentachlorobenzene and hexachlorobenzene. Under optimized chromatographic conditions,good chromatographic separation result was gotten for 12 chlorobenzenes target compounds which were in methanol matrix mixed solution.It avoided the influence of the impurities of isomers and homologues in the raw materials on the results of purity determination due to the poor separation between the impurities and the target compounds.
The research of purity chromatography measurement method( HPLC-DAD and GC-FID) for 12 kinds of chlorobenzenes raw materials which were used to prepare chlorobenzenes solution reference material was introduced. It contains monochlorobenzene,1,2-dichlorobenzene,1,4-dichlorobenzene,1,3-dichlorobenzene 1,2,3-trichlorobenzene,1,2,4-trichlorobenzene,1,3,5-trichlorobenzene,1,2,3,4-tetrachlorobenzene,1,2,4,5-tetrachlorobenzene,1,2,3,5-tetrachlorobenzene,pentachlorobenzene and hexachlorobenzene. Under optimized chromatographic conditions,good chromatographic separation result was gotten for 12 chlorobenzenes target compounds which were in methanol matrix mixed solution.It avoided the influence of the impurities of isomers and homologues in the raw materials on the results of purity determination due to the poor separation between the impurities and the target compounds.
引文
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[5]辛梅华,李明春,徐金瑞,等.工业废水中氯苯等6种有机污染物的RP-HPLC分离检测[J].环境工程,2003,21(4):53-54.
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[7]温左钧,赖永忠.顶空固相微萃取联合GC-MS检测水中10种氯苯系物[J].广州化工,2011,39(10):112-115.
[8]奚晔,郑嵘,詹铭.顶空气相色谱法测定水中11种氯苯类化合物及六氯丁二烯[J].上海预防医学,2017,29(1):44-53.
[9]刘静,曾兴宇,烟卫.毛细管气相色谱法测定水中氯苯类化合物和有机氯农药[J].化学试剂,2011,33(4):334-336.
[10]田艳,黄宁,梁柳玲,等.吹扫捕集气相色谱-质谱法测定地表水中12种氯苯类有机物[J].中国环境监测,2014,30(3):111-114.
[2]中国国家标准化管理委员会.生活饮用水卫生标准:GB 5749—2006[S].北京:中国标准出版社,2007.
[3]王硕,戴炳业,张岩.氯苯类化合物测定方法的研究现状[J].天津科技大学学报,2008,23(1):83-86.
[4]乐小亮,何娟,肖娇.固相萃取高效液相色谱法测定废水中4种氯苯类有机污染物[J].安徽农业科学,2013,41(13):5 894-5 896.
[5]辛梅华,李明春,徐金瑞,等.工业废水中氯苯等6种有机污染物的RP-HPLC分离检测[J].环境工程,2003,21(4):53-54.
[6]杨丽莉,母应锋,姚诚,等.毛细管气相色谱法测定水中氯苯类化合物[J].环境监测管理与技术,2007,19(2):34-38.
[7]温左钧,赖永忠.顶空固相微萃取联合GC-MS检测水中10种氯苯系物[J].广州化工,2011,39(10):112-115.
[8]奚晔,郑嵘,詹铭.顶空气相色谱法测定水中11种氯苯类化合物及六氯丁二烯[J].上海预防医学,2017,29(1):44-53.
[9]刘静,曾兴宇,烟卫.毛细管气相色谱法测定水中氯苯类化合物和有机氯农药[J].化学试剂,2011,33(4):334-336.
[10]田艳,黄宁,梁柳玲,等.吹扫捕集气相色谱-质谱法测定地表水中12种氯苯类有机物[J].中国环境监测,2014,30(3):111-114.