UPLC法测定灵芝子实体中11种三萜酸含量
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摘要
灵芝三萜酸具有抗肿瘤、免疫调节、解毒、抗炎及保肝等多种药理活性。本研究建立了超高效液相色谱法同时测定灵芝子实体中11种三萜酸的方法。采用ACQUITY UPLC HSS T3(2.1 mm×50 mm,1.7 m)色谱柱,以乙腈0.03%磷酸水溶液为流动相梯度洗脱,流速0.5 mL/min,柱温35℃。赤芝酸C、赤芝酸N、灵芝酸G、灵芝烯酸B、灵芝酸B、脱乙酰灵芝酸F、灵芝酸A、赤芝酸A、灵芝酸D、赤芝酸D、灵芝酸F在0.004～0.06 mg/mL内有良好的线性关系,加样回收率均高于95.0%,RSD均小于5.0%。本方法简便、稳定,可在16 min内完成11种三萜酸的定量分析,为灵芝质量评价提供了参考依据。
Triterpenoid Acids of Ganoderma lucidum have a variety of pharmacological activities such as anti-tumor, immunomodulatory,detoxification, anti-inflammatory and liver preservation. A method for the simultaneous determination of 11 triterpenoid acids in sporophore of G.lucidum is described, in which ultra performance liquid chromatography are used. A acquity uplc HSS T3(2.1 mm×50 mm,1.7 m)column kept at 35 ℃ and eluted with acetonitrile 0.03% phospholic acid solution at a flow rate of 0.5 mL/min was used for the chromatographic separation. The column temperature was 35 ℃. The concentration range of Lucidenic acid C, Lucidenic acid N, Ganoderic acid G, Ganoderenic acid B, Ganoderic acid B, Deacetyl ganoderic acid F, Ganoderic acid A, Lucidenic acid A, Ganoderic acid D, Lucidenic acid D and Ganoderic acid F was in from 0.004 to 0.06 mg/mL and the peak area showed good linearity. Their recoveries were more than 95.0%,with RSD being less than 5.0%. The method is convenient and reliable, it could be used for the quantitative analysis of eleven triterpenoid acids with in 16 min. It provide reference for quality evaluation of G.lucidum.
Triterpenoid Acids of Ganoderma lucidum have a variety of pharmacological activities such as anti-tumor, immunomodulatory,detoxification, anti-inflammatory and liver preservation. A method for the simultaneous determination of 11 triterpenoid acids in sporophore of G.lucidum is described, in which ultra performance liquid chromatography are used. A acquity uplc HSS T3(2.1 mm×50 mm,1.7 m)column kept at 35 ℃ and eluted with acetonitrile 0.03% phospholic acid solution at a flow rate of 0.5 mL/min was used for the chromatographic separation. The column temperature was 35 ℃. The concentration range of Lucidenic acid C, Lucidenic acid N, Ganoderic acid G, Ganoderenic acid B, Ganoderic acid B, Deacetyl ganoderic acid F, Ganoderic acid A, Lucidenic acid A, Ganoderic acid D, Lucidenic acid D and Ganoderic acid F was in from 0.004 to 0.06 mg/mL and the peak area showed good linearity. Their recoveries were more than 95.0%,with RSD being less than 5.0%. The method is convenient and reliable, it could be used for the quantitative analysis of eleven triterpenoid acids with in 16 min. It provide reference for quality evaluation of G.lucidum.
引文
[1]国家药典委员会.中国药典:一部[K].北京:中国医药科技出版社,2015:188.
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[3] MIN B S, GAO J J, Nakamura N, et al.Triterpenes from the spo-res of Ganoderma lucidum and their cytotoxicity against Meth-A and LLC tumor cells[J]. Chem Pham Bull, 2000,48(7):1026-1033.
[4]罗俊,林志彬.灵芝三萜类化合物药理作用研究进展[J].药学学报,2002,37(7):574-578.
[5]董虹玲,夏广萍,赵娜夏.HPLC法测定灵芝子实体和孢子粉中灵芝酸C2、灵芝酸G和灵芝酸A[J].现代药物与临床,2013,28(1):41-43.
[6]赵佳,陈晓辉,毕开顺. HPLC同时测定赤芝中4种三萜酸的含量[J].中国中药杂志,2009, 34(17):2220-2222.
[7]路娟,秦加长,陈萍.六种不同来源灵芝的质量差异性研究[J].中医药信息,2012, 29(2):17-20.
[2]梁英娇,楚桐丽,丁平.赤芝中三萜类成分及其药理作用研究进展[J].中药新药与临床药理, 2007,18(2):168.
[3] MIN B S, GAO J J, Nakamura N, et al.Triterpenes from the spo-res of Ganoderma lucidum and their cytotoxicity against Meth-A and LLC tumor cells[J]. Chem Pham Bull, 2000,48(7):1026-1033.
[4]罗俊,林志彬.灵芝三萜类化合物药理作用研究进展[J].药学学报,2002,37(7):574-578.
[5]董虹玲,夏广萍,赵娜夏.HPLC法测定灵芝子实体和孢子粉中灵芝酸C2、灵芝酸G和灵芝酸A[J].现代药物与临床,2013,28(1):41-43.
[6]赵佳,陈晓辉,毕开顺. HPLC同时测定赤芝中4种三萜酸的含量[J].中国中药杂志,2009, 34(17):2220-2222.
[7]路娟,秦加长,陈萍.六种不同来源灵芝的质量差异性研究[J].中医药信息,2012, 29(2):17-20.