高血压大鼠肝脏组织及外周血白细胞端粒长度及其相关性
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摘要
目的探讨高血压大鼠外周血白细胞和肝脏组织端粒相对长度及二者相关性。方法将16只SD雄性大鼠随机分为高血压组和对照组各8只。高血压组采用双侧肾动脉后支结扎以及结扎后喂食8%NaCl饲料的方法构建高血压大鼠模型,对照组用标准饲料进行喂养。饲养4个月后比较两组大鼠肝脏组织及外周血白细胞端粒长度,并分析高血压大鼠外周血白细胞端粒与肝脏组织端粒的关系。结果高血压组大鼠肝脏组织及外周血白细胞端粒长度均短于对照组(均P<0.05),高血压组大鼠外周血白细胞与肝脏组织端粒长度呈正相关(P<0.05)。结论高血压大鼠外周血白细胞及肝脏组织端粒长度明显缩短,两者存在一定相关性。
Objective To investigate the relative telomere lengths of peripheral blood leukocytes and liver tissues in hypertensive rats and their correlation. Methods Sixteen male SD rats were randomly divided into hypertension group and control group,with 8 rats in each group. The hypertension group was given 8% Na Cl diet after bilateral posterior renal artery ligation to develop the hypertensive rat model,and the control group was given the normal diet. After four months of feeding,the telomere lengths of liver tissues and peripheral blood leukocytes of rats were compared between the two groups,and the relationship between peripheral blood leukocyte and liver tissue telomere lengths was analyzed. Results The telomere lengths of liver tissues and peripheral blood leukocytes of the rats in the hypertension group were shorter than those in the control group(all P < 0. 05),and there was a positive correlation between peripheral blood leukocyte and liver tissue telomere lengths of the rats in the hypertension group(P < 0. 05). Conclusion The telomere lengths of peripheral blood leukocytes and liver tissues decrease significantly in the hypertensive rats,and they show a certain correlation.
Objective To investigate the relative telomere lengths of peripheral blood leukocytes and liver tissues in hypertensive rats and their correlation. Methods Sixteen male SD rats were randomly divided into hypertension group and control group,with 8 rats in each group. The hypertension group was given 8% Na Cl diet after bilateral posterior renal artery ligation to develop the hypertensive rat model,and the control group was given the normal diet. After four months of feeding,the telomere lengths of liver tissues and peripheral blood leukocytes of rats were compared between the two groups,and the relationship between peripheral blood leukocyte and liver tissue telomere lengths was analyzed. Results The telomere lengths of liver tissues and peripheral blood leukocytes of the rats in the hypertension group were shorter than those in the control group(all P < 0. 05),and there was a positive correlation between peripheral blood leukocyte and liver tissue telomere lengths of the rats in the hypertension group(P < 0. 05). Conclusion The telomere lengths of peripheral blood leukocytes and liver tissues decrease significantly in the hypertensive rats,and they show a certain correlation.
引文
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[15]Friedrich U,Griese E,Schwab M,et al.Telomere length in different tissues of elderly patients[J].Mech Ageing Dev,2000,119(3):89-99.
[16]陈宇,刘继斌,刘鹏,等.人颈动脉粥样硬化斑块组织及外周血白细胞相对端粒长度的检测及相关性分析[J].中国分子心脏病学杂志,2012,12(1):27-31.
[2]邹傲.非酒精性脂肪性肝病的相关危险因素研究进展[J].胃肠病学和肝病学杂志,2012,21(1):92-94.
[3]陈颖基.肝脏衰老与非酒精性脂肪性肝病患者肝脏炎症,纤维化进展相关性的研究[D].杭州:浙江中医药大学,2016.
[4]Eldawoody H,Shimizu H,Kimura N,et al.Simplified experimental cerebral aneurysm model in rats:comprehensive evaluation of induced aneurysms and arterial changes in the circle of Willis[J].Brain Res,2009,1300:159-168.
[5]O'Callaghan N,Dhillon V,Thomas P,et al.A quantitative real-time PCR method for absolute telomere length[J].Biotechniques,2008,44(6):807-809.
[6]钟英成,罗朋立,靳国恩,等.荧光实时定量PCR检测端粒长度[J].临床和实验医学杂志,2008,7(4):14-16.
[7]付浩,孙圣凯,陈孝储,等.高血压大鼠线粒体DNA拷贝数与端粒长度的相关性研究[J].中国医药导报,2016,13(31):33-36.
[8]Jansen PL.Nonalcoholic steatohepatitis:diagnosis,pathogenesis,treatment and prognosis[J].Ned Tijdschr Geneeskd,2005,149(6):289-294.
[9]Blackburn EH.Telomeres[J].Trends Biochem Sci,1991,16(10):378-381.
[10]Allsopp R.Telomere length and i PSC re-programming:survival of the longest[J].Cell Res,2012,22(4):614-615.
[11]周璇,黄河浪,吴磊.端粒及其长度改变与人衰老关系的研究进展[J].中国老年学,2015,35(5):1 436-1 438.
[12]Sakaguchi S,Takahashi S,Sasaki TA,et al.Progression of alcoholic and non-alcoholic steatohepatitis:common metabolic aspects of innate immune system and oxidative stress[J].Drug Metab Pharmacokinet,2011,26(1):30-46.
[13]Demissie S,Levy D,Benjamin EJ,et al.Insulin resistance,oxidative stress,hypertension,and leukocyte telomere length in men from the Framingham Heart Study[J].Aging Cell,2006,5(4):325-330.
[14]Briones AM,Touyz RM.Oxidative stress and hypertension:current concepts[J].Curr Hypertens Rep,2010,12(2):135-142.
[15]Friedrich U,Griese E,Schwab M,et al.Telomere length in different tissues of elderly patients[J].Mech Ageing Dev,2000,119(3):89-99.
[16]陈宇,刘继斌,刘鹏,等.人颈动脉粥样硬化斑块组织及外周血白细胞相对端粒长度的检测及相关性分析[J].中国分子心脏病学杂志,2012,12(1):27-31.