哺乳动物体内、外生产胚胎的表观遗传差异研究进展
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摘要
体外胚胎生产作为一项高效的辅助生殖技术,对优质种质资源的保存利用及遗传改良具有重要意义。但与体内生产胚胎相比,体外生产胚胎在培养过程中出现生长发育缓慢、卵裂率低、凋亡比例增加等问题,移植后伴随着胎盘肥大、孕期延长等问题,还可能出现早产、出生体重降低、巨胎综合征(LOS)等,这与基因表达异常和表观遗传修饰异常有重要关系。文章简单回顾了近年来表观修饰学在体内、外生产胚胎的研究进展,主要介绍了印记基因的表观修饰、DNA甲基化及组蛋白乙酰化在哺乳动物体内、外生产胚胎之间的差异,简述了微阵列技术在体内、外生产胚胎之间的应用,以期找到导致体内、外胚胎质量差异的关键印记基因及其作用途径,探讨体外生产胚胎质量低于体内胚胎的原因,进而改善体外胚胎生产体系。
As an efficient assisted reproductive technology,in vitro embryo production is of great significance to the conservation and effective utilization of germplasm resources and genetic improvement.However,compared with embryos produced in vivo,the embryos produced in vitro develop slowly,the cleavage rate is low and the rate of apoptosis increases in the process of culture,which are accompanied by placental hypertrophy and prolonged pregnancy after transplantation.Preterm delivery,low birth weight,and macrosomia syndrome(LOS)occur after birth which are closely related to abnormal gene expression and epigenetic modification.The progress of epigenetic modification in the production of embryos in vivo and in vitro was briefly reviewed in this paper.The differences of epigenetic modification of imprinted genes,DNA methylation and histone acetylation between mammalian embryos in vivo and in vitro were introduced.The application of microarray technique in the production of embryos in vitro and in vivo was briefly described in order to obtain the key imprinted genes that lead to the difference of embryo quality betweenin vivoand in vitro,and explored the reasons why the quality of embryos produced in vitro was lower than that of in vivo,and then improved the in vitro embryo production system.
As an efficient assisted reproductive technology,in vitro embryo production is of great significance to the conservation and effective utilization of germplasm resources and genetic improvement.However,compared with embryos produced in vivo,the embryos produced in vitro develop slowly,the cleavage rate is low and the rate of apoptosis increases in the process of culture,which are accompanied by placental hypertrophy and prolonged pregnancy after transplantation.Preterm delivery,low birth weight,and macrosomia syndrome(LOS)occur after birth which are closely related to abnormal gene expression and epigenetic modification.The progress of epigenetic modification in the production of embryos in vivo and in vitro was briefly reviewed in this paper.The differences of epigenetic modification of imprinted genes,DNA methylation and histone acetylation between mammalian embryos in vivo and in vitro were introduced.The application of microarray technique in the production of embryos in vitro and in vivo was briefly described in order to obtain the key imprinted genes that lead to the difference of embryo quality betweenin vivoand in vitro,and explored the reasons why the quality of embryos produced in vitro was lower than that of in vivo,and then improved the in vitro embryo production system.
引文
[1]YAMANAKA K I,YAMASHITA K,KHAYUN H,et al.Normal DNA methylation status in sperm from a somatic cell cloned bull and their fertilized embryos[J].Animal Science Journal,2018,89(10):1406-1414.
[2]GEORGE P C.2015statistics of embryo collection and transfer in domestic farm animals[J].International Embryo Technology Society,2016,34(4):10-25.
[3]SIRARD M A.The influence of in vitro fertilization and embryo culture on the embryo epigenetic constituents and the possible consequences in the bovine model[J].Journal of Developmental Origins of Health and Disease,2017,8(4):411-417.
[4]TAN K,AN L,MIAO K,et al.Impaired imprinted Xchromosome inactivation is responsible for the skewed sex ratio following in vitro fertilization[J].Proceedings of The National Academy of Sciences,2016,113(12):3197-3202.
[5]RIZOS D,LONERGAN P,BOLAND M P,et al.Analysis of differential messenger RNA expression between bovine blastocysts produced in different culture systems:Implications for blastocyst quality[J].Biology Reproduction,2002,66(3):589-595.
[6]ZULLO G,CANDITIIS C D,PERO M E,et al.Crocetin improves the quality of in vitro-produced bovine embryos:Implications for blastocyst development,cryotolerance,and apoptosis[J].Theriogenology,2016,86(8):1879-1885.
[7]CHEN M,NI J,CHANG H C,et al.CCDC62/ER-AP75functions as a coactivator to enhance estrogen receptor beta-mediated transactivation and target gene expression in prostate cancer cells[J].Carcinogenesis,2009,30(5):841-850.
[8]NIE J,AN L,MIAI K,et al.Comparative analysis of dynamic proteomic profiles between in vivo and in vitro produced mouse embryos during postimplantation period[J].Journal of Proteome Research,2013,12(9):3843-3856.
[9]LONERGAN P,EVANS A C,BOLAND E,et al.Pregnancy and fetal characteristics after transfer of vitrified in vivo and cloned bovine embryos[J].Theriogenology,2007,68(8):1128-1137.
[10]DEBAUN M R,NIEMITZ E L,FEINBERG A P.Association of in vitro fertilization with BeckwithWiedemann syndrome and epigenetic alterations of LIT1and H19[J].The American Journal Human Genetics,2003,72(1):156-160.
[11]HIENDLEDER S,WIRTZ M,MUND C,et al.Tissue-specific effects of in vitro fertilization procedures on genomic cytosine methylation levels in overgrown and normal sized bovine fetuses[J].Biology Reproduction,2006,75(1):17-23.
[12]URREGO R,RODRIGUEZ O N,NIEMANN H.Epigenetic disorders and altered gene expression after use of assisted reproductive technologies in domestic cattle[J].Epigenetics,2014,9(6):803-815.
[13]WADDINGTON C H.The epigenotype[J].Endeavour,1942,1:18-20.
[14]KELSEY G,STEGLE O,REIK W.Single-cell epigenomics:Recording the past and predicting the future[J].Science,2017,358(6359):69-75.
[15]FANG X,ZHAO Z,YU H,et al.Comparative genome-wide methylation analysis of longissimus dorsi muscles between Japanese Black(Wagyu)and Chinese Red Steppes cattle[J].PLoS One,2017,12(8):e0182492.
[16]杨慧,张昌军,刁红录.组蛋白乙酰化与哺乳动物生殖[J].中国细胞生物学学报,2017,39(4):523-528.YANG H,ZHANG C J,DIAO H L.Histone acetylation and mammalian reproduction[J].Chinese Journal of Cell Biology,2017,39(4):523-528.(in Chinese)
[17]李广栋,崔炜,刘国世,等.NLRP7基因在母源印记中的研究进展[J].中国畜牧兽医,2018,45(4):1002-1008.LI G D,CUI W,LIU G S,et al.Research progress on NLRP7gene in maternal imprining[J].China Animal Husbandry&Veterinary Medicine,2018,45(4):1002-1008.(in Chinese)
[18]LI T,VU T H,ULANER G A,et al.IVF results in de novo DNA methylation and histone methylation at an Igf2-H19imprinting epigenetic switch[J].Molecular Human Reproduction,2005,11(9):631-640.
[19]MANN M R,CHUNG Y G,NOLEN L D,et al.Disruption of imprinted gene methylation and expression in cloned preimplantation stage mouse embryos[J].Biology Reproduction,2003,69(3):902-914.
[20]HEINZMANN J,HANSMANN T,HERRMANN D,et al.Epigenetic profile of developmentally important genes in bovine oocytes[J].Molecular Reproduction Development,2011,78(3):188-201.
[21]ZHAN Q,QI X,WANG N,et al.Altered methylations of H19,Snrpn,Mest and Peg3are reversible by developmental reprogramming in kidney tissue of ICSI-derived mice[J].Scientific Reports,2017,7(1):11936.
[22]FARIN C E,ALEXANDER J E,FARIN P W.Expression of messenger RNAs for insulin-like growth factors and their receptors in bovine fetuses at early gestation from embryos produced in vivo or in vitro[J].Theriogenology,2010,74(7):1288-1295.
[23]YOUNG L E,FERNANDES K,MCEVOY T G,et al.Epigenetic change in IGF2Ris associated with fetal overgrowth after sheep embryo culture[J].Nature Genetics,2001,27(2):153-154.
[24]SUZUKI J,THERRIEN J J,FILION F,et al.In vitro culture and somatic cell nuclear transfer affect imprinting of SNRPN gene in pre-and post-implantation stages of development in cattle[J].BMC Development Biology,2009,9:9.
[25]SMITH L C,THERRIEN J,FILION F.Epigenetic consequences of artificial reproductive technologies to the bovine imprinted genes SNRPN,H19/IGF2,and IGF2R[J].Frontiers in Genetics,2015,6:58.
[26]RIEUSSET A,SCHALLER F,UNMEHOPA U,et al.Stochastic loss of silencing of the imprinted Ndn/NDN allele,in a mouse model and humans with Prader-Willi syndrome,has functional consequences[J].PLoS Genetics,2013,9(9):e1003752.
[27]RIBARSKA T,GOERING W,DROOP J,et al.Deregulation of an imprinted gene network in prostate cancer[J].Epigenetics,2014,9(5):704-717.
[28]HOU J,LIU L,LEI T,et al.Genomic DNA methylation patterns in bovine preimplantation embryos derived from in vitro fertilization[J].Science China Life Sciences,2007,50(1):56-61.
[29]SU J,WANG Y,XING X,et al.Genome-wide analysis of DNA methylation in bovine placentas[J].BMC Genomics,2014,15:12-22.
[30]SONG S,GHOSH J,MAINIG M,et al.DNA methylation differences between in vitro-and in vivo-conceived children are associated with ART procedures rather than infertility[J].Clinical Epigenetics,2015,7:41-50.
[31]王静,胡雪峰.DNA甲基化与癌症之间关系研究概述[J].生物学教学,2018,43(2):5-7.WANG J,HU X F.Review of the relationship between DNA methylation and cancer[J].Biology Teaching,2018,43(2):5-7.(in Chinese)
[32]URREGO R,BERNAL-ULLOA S M,CHAVARRIA N A,et al.Satellite DNA methylation status and expression of selected genes in Bos indicus blastocysts produced in vivo and in vitro[J].Zygote,2017,25(2):131-140.
[33]CIRIO M C,RATNAM S,DING F,et al.Preimplantation expression of the somatic form of Dnmt1suggests a role in the inheritance of genomic imprints[J].BMC Development Biology,2008,8:9-22.
[34]刘静,吴坤陆,朱敏,等.分子生物学实验指导[M].长沙:中南大学出版社,2015.LIU J,WU K L,ZHU M,et al.Molecular Biology Experimental Guidance[M].Changsha:Central South University Press,2015.(in Chinese)
[35]HOU L,MA F,YANG J,et al.Effects of histone deacetylase inhibitor oxamflatin on in vitro porcine somatic cell nuclear transfer embryos[J].Cell Reprogram,2014,16(4):253-265.
[36]CAO Z,HONG R,DING B,et al.TSA and BIX-01294induced normal DNA and histone methylation and increased protein expression in porcine somatic cell nuclear transfer embryos[J].PLoS One,2017,12(1):e0169092.
[37]ALSALIM H,JAFARPOUR F,VASH N T,et al.Effect of DNA and histone methyl transferase inhibitors on outcomes of buffalo-bovine interspecies somatic cell nuclear transfer[J].Cellular Reprogramming,2018,20(4):256-267.
[38]FU Y,XU J J,SUN X L,et al.Function of JARID2in bovines during early embryonic development[J].PeerJ,2017,5(12):4189-4206.
[39]CORCORAN D,FAIR T,PARK S,et al.Suppressed expression of genes involved in transcription and translation in in vitro compared with in vivo cultured bovine embryos[J].Reproduction(Cambridge,England),2006,131(4):651-660.
[40]MARTHA P G,CENA M,LYNETTE A C,et al.Identification of differentially expressed genes in individual bovine preimplantation embryos produced by nuclear transfer:Improper reprogramming of genes required for development[J].Biology Reproduction,2005,72(3):546-555.
[41]DRIVER A M,PENAGARICANO F,HUANG W,et al.RNA-Seq analysis uncovers transcriptomic variations between morphologically similar in vivo-and in vitro-derived bovine blastocysts[J].BMC Genomics,2012,13:118-126.
[2]GEORGE P C.2015statistics of embryo collection and transfer in domestic farm animals[J].International Embryo Technology Society,2016,34(4):10-25.
[3]SIRARD M A.The influence of in vitro fertilization and embryo culture on the embryo epigenetic constituents and the possible consequences in the bovine model[J].Journal of Developmental Origins of Health and Disease,2017,8(4):411-417.
[4]TAN K,AN L,MIAO K,et al.Impaired imprinted Xchromosome inactivation is responsible for the skewed sex ratio following in vitro fertilization[J].Proceedings of The National Academy of Sciences,2016,113(12):3197-3202.
[5]RIZOS D,LONERGAN P,BOLAND M P,et al.Analysis of differential messenger RNA expression between bovine blastocysts produced in different culture systems:Implications for blastocyst quality[J].Biology Reproduction,2002,66(3):589-595.
[6]ZULLO G,CANDITIIS C D,PERO M E,et al.Crocetin improves the quality of in vitro-produced bovine embryos:Implications for blastocyst development,cryotolerance,and apoptosis[J].Theriogenology,2016,86(8):1879-1885.
[7]CHEN M,NI J,CHANG H C,et al.CCDC62/ER-AP75functions as a coactivator to enhance estrogen receptor beta-mediated transactivation and target gene expression in prostate cancer cells[J].Carcinogenesis,2009,30(5):841-850.
[8]NIE J,AN L,MIAI K,et al.Comparative analysis of dynamic proteomic profiles between in vivo and in vitro produced mouse embryos during postimplantation period[J].Journal of Proteome Research,2013,12(9):3843-3856.
[9]LONERGAN P,EVANS A C,BOLAND E,et al.Pregnancy and fetal characteristics after transfer of vitrified in vivo and cloned bovine embryos[J].Theriogenology,2007,68(8):1128-1137.
[10]DEBAUN M R,NIEMITZ E L,FEINBERG A P.Association of in vitro fertilization with BeckwithWiedemann syndrome and epigenetic alterations of LIT1and H19[J].The American Journal Human Genetics,2003,72(1):156-160.
[11]HIENDLEDER S,WIRTZ M,MUND C,et al.Tissue-specific effects of in vitro fertilization procedures on genomic cytosine methylation levels in overgrown and normal sized bovine fetuses[J].Biology Reproduction,2006,75(1):17-23.
[12]URREGO R,RODRIGUEZ O N,NIEMANN H.Epigenetic disorders and altered gene expression after use of assisted reproductive technologies in domestic cattle[J].Epigenetics,2014,9(6):803-815.
[13]WADDINGTON C H.The epigenotype[J].Endeavour,1942,1:18-20.
[14]KELSEY G,STEGLE O,REIK W.Single-cell epigenomics:Recording the past and predicting the future[J].Science,2017,358(6359):69-75.
[15]FANG X,ZHAO Z,YU H,et al.Comparative genome-wide methylation analysis of longissimus dorsi muscles between Japanese Black(Wagyu)and Chinese Red Steppes cattle[J].PLoS One,2017,12(8):e0182492.
[16]杨慧,张昌军,刁红录.组蛋白乙酰化与哺乳动物生殖[J].中国细胞生物学学报,2017,39(4):523-528.YANG H,ZHANG C J,DIAO H L.Histone acetylation and mammalian reproduction[J].Chinese Journal of Cell Biology,2017,39(4):523-528.(in Chinese)
[17]李广栋,崔炜,刘国世,等.NLRP7基因在母源印记中的研究进展[J].中国畜牧兽医,2018,45(4):1002-1008.LI G D,CUI W,LIU G S,et al.Research progress on NLRP7gene in maternal imprining[J].China Animal Husbandry&Veterinary Medicine,2018,45(4):1002-1008.(in Chinese)
[18]LI T,VU T H,ULANER G A,et al.IVF results in de novo DNA methylation and histone methylation at an Igf2-H19imprinting epigenetic switch[J].Molecular Human Reproduction,2005,11(9):631-640.
[19]MANN M R,CHUNG Y G,NOLEN L D,et al.Disruption of imprinted gene methylation and expression in cloned preimplantation stage mouse embryos[J].Biology Reproduction,2003,69(3):902-914.
[20]HEINZMANN J,HANSMANN T,HERRMANN D,et al.Epigenetic profile of developmentally important genes in bovine oocytes[J].Molecular Reproduction Development,2011,78(3):188-201.
[21]ZHAN Q,QI X,WANG N,et al.Altered methylations of H19,Snrpn,Mest and Peg3are reversible by developmental reprogramming in kidney tissue of ICSI-derived mice[J].Scientific Reports,2017,7(1):11936.
[22]FARIN C E,ALEXANDER J E,FARIN P W.Expression of messenger RNAs for insulin-like growth factors and their receptors in bovine fetuses at early gestation from embryos produced in vivo or in vitro[J].Theriogenology,2010,74(7):1288-1295.
[23]YOUNG L E,FERNANDES K,MCEVOY T G,et al.Epigenetic change in IGF2Ris associated with fetal overgrowth after sheep embryo culture[J].Nature Genetics,2001,27(2):153-154.
[24]SUZUKI J,THERRIEN J J,FILION F,et al.In vitro culture and somatic cell nuclear transfer affect imprinting of SNRPN gene in pre-and post-implantation stages of development in cattle[J].BMC Development Biology,2009,9:9.
[25]SMITH L C,THERRIEN J,FILION F.Epigenetic consequences of artificial reproductive technologies to the bovine imprinted genes SNRPN,H19/IGF2,and IGF2R[J].Frontiers in Genetics,2015,6:58.
[26]RIEUSSET A,SCHALLER F,UNMEHOPA U,et al.Stochastic loss of silencing of the imprinted Ndn/NDN allele,in a mouse model and humans with Prader-Willi syndrome,has functional consequences[J].PLoS Genetics,2013,9(9):e1003752.
[27]RIBARSKA T,GOERING W,DROOP J,et al.Deregulation of an imprinted gene network in prostate cancer[J].Epigenetics,2014,9(5):704-717.
[28]HOU J,LIU L,LEI T,et al.Genomic DNA methylation patterns in bovine preimplantation embryos derived from in vitro fertilization[J].Science China Life Sciences,2007,50(1):56-61.
[29]SU J,WANG Y,XING X,et al.Genome-wide analysis of DNA methylation in bovine placentas[J].BMC Genomics,2014,15:12-22.
[30]SONG S,GHOSH J,MAINIG M,et al.DNA methylation differences between in vitro-and in vivo-conceived children are associated with ART procedures rather than infertility[J].Clinical Epigenetics,2015,7:41-50.
[31]王静,胡雪峰.DNA甲基化与癌症之间关系研究概述[J].生物学教学,2018,43(2):5-7.WANG J,HU X F.Review of the relationship between DNA methylation and cancer[J].Biology Teaching,2018,43(2):5-7.(in Chinese)
[32]URREGO R,BERNAL-ULLOA S M,CHAVARRIA N A,et al.Satellite DNA methylation status and expression of selected genes in Bos indicus blastocysts produced in vivo and in vitro[J].Zygote,2017,25(2):131-140.
[33]CIRIO M C,RATNAM S,DING F,et al.Preimplantation expression of the somatic form of Dnmt1suggests a role in the inheritance of genomic imprints[J].BMC Development Biology,2008,8:9-22.
[34]刘静,吴坤陆,朱敏,等.分子生物学实验指导[M].长沙:中南大学出版社,2015.LIU J,WU K L,ZHU M,et al.Molecular Biology Experimental Guidance[M].Changsha:Central South University Press,2015.(in Chinese)
[35]HOU L,MA F,YANG J,et al.Effects of histone deacetylase inhibitor oxamflatin on in vitro porcine somatic cell nuclear transfer embryos[J].Cell Reprogram,2014,16(4):253-265.
[36]CAO Z,HONG R,DING B,et al.TSA and BIX-01294induced normal DNA and histone methylation and increased protein expression in porcine somatic cell nuclear transfer embryos[J].PLoS One,2017,12(1):e0169092.
[37]ALSALIM H,JAFARPOUR F,VASH N T,et al.Effect of DNA and histone methyl transferase inhibitors on outcomes of buffalo-bovine interspecies somatic cell nuclear transfer[J].Cellular Reprogramming,2018,20(4):256-267.
[38]FU Y,XU J J,SUN X L,et al.Function of JARID2in bovines during early embryonic development[J].PeerJ,2017,5(12):4189-4206.
[39]CORCORAN D,FAIR T,PARK S,et al.Suppressed expression of genes involved in transcription and translation in in vitro compared with in vivo cultured bovine embryos[J].Reproduction(Cambridge,England),2006,131(4):651-660.
[40]MARTHA P G,CENA M,LYNETTE A C,et al.Identification of differentially expressed genes in individual bovine preimplantation embryos produced by nuclear transfer:Improper reprogramming of genes required for development[J].Biology Reproduction,2005,72(3):546-555.
[41]DRIVER A M,PENAGARICANO F,HUANG W,et al.RNA-Seq analysis uncovers transcriptomic variations between morphologically similar in vivo-and in vitro-derived bovine blastocysts[J].BMC Genomics,2012,13:118-126.