基于寻峰算法的荧光检测设备的设计与实现
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摘要
为了较简单、精确地获得C反应蛋白(CRP)检测中T峰和C峰的位置,设计了一种小型的荧光检测设备,并提出一种新的寻峰算法,即平移窗口寻峰算法。该检测设备包括光路、电路等的设计及优化。通过单光路结构的设计,保证设备结构紧凑、整体体积较小;通过内环电流反馈和外环光强反馈,使得LED激发光输出稳定。对CRP的检测实验表明,平移窗口寻峰算法操作简单,获得T峰和C峰的位置精确度相比于一般多项式拟合法分别提升50.0%和52.9%,具有较高的精度。
A small-sized fluorescence detection device is designed to simply and accurately acquire the location of T-peak and C-peak in C-reactive protein(CRP)detection,and a window shifting peak-searching algorithm is proposed. This detection device includes the design and optimization of optical path and circuit. The design of single optical path structure can maintain the compact structure and small overall size of the device. The inner current feedback and outer light intensity feedback can make the output light excited by LED stable. The detection experiment results of CRP show that the window shifting peak-searching algorithm has easy operation and high precision,and the obtained location accuracy of T-peak and C-peak is increased by 50.0% and 52.9% respectively than that of the general polynomial fitting method.
A small-sized fluorescence detection device is designed to simply and accurately acquire the location of T-peak and C-peak in C-reactive protein(CRP)detection,and a window shifting peak-searching algorithm is proposed. This detection device includes the design and optimization of optical path and circuit. The design of single optical path structure can maintain the compact structure and small overall size of the device. The inner current feedback and outer light intensity feedback can make the output light excited by LED stable. The detection experiment results of CRP show that the window shifting peak-searching algorithm has easy operation and high precision,and the obtained location accuracy of T-peak and C-peak is increased by 50.0% and 52.9% respectively than that of the general polynomial fitting method.
引文
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[3]刘翔,杜民,李玉榕,等.荧光免疫层析定量检测系统的设计与实现[J].电子测量与仪器学报,2013,27(9):859-866.LIU Xiang,DU Min,LI Yurong,et al. Design of fluorescence immune-chromatographic quantitative detection system[J]. Journal of electronic measurement and instrument,2013,27(9):859-866.
[4]张晓慧,李光韬,张卓莉.C反应蛋白与超敏C反应蛋白的检测及其临床意义[J].中华临床免疫和变态反应杂志,2011,5(1):74-79.ZHANG Xiaohui,LI Guangtao,ZHANG Zhuoli. Clinical significances of C-reactive protein and hypersensitive C-reactive protein[J]. Chinese journal of allergy&clinical immunology,2011,5(1):74-79.
[5]韩屏,周祖德.高速高精光栅解调器的反馈式相对寻峰算法[J].武汉理工大学学报(信息与管理工程版),2011,33(1):10-12.HAN Ping,ZHOU Zude. Feedback relative peak-detection algorithm for high-speed and high-precision grating demodulator[J]. Journal of Wuhan University of Technology(information&management engineering),2011,33(1):10-12.
[6]刘泉,王欢.高速光纤光栅解调的寻峰算法研究[J].武汉理工大学学报,2010,32(6):59-61.LIU Quan,WANG Huan. Research on the peak-detection algorithm in the high-frequency demodulation for the fiber Bragg grating[J]. Journal of Wuhan University of Technology,2010,32(6):59-61.
[7] LEE H W,PARK H J,LEE J H,et al. Accuracy improvement in peak positioning of spectrally distorted fiber Bragg grating sensors by Gaussian curve fitting[J]. Applied optics,2007,46(12):2205-2208.
[8]马永杰,云文霞.遗传算法研究进展[J].计算机应用研究,2012,29(4):1201-1206.MA Yongjie,YUN Wenxia. Research progress of genetic algorithm[J]. Application research of computers,2012,29(4):1201-1206.
[9] ASAOKA T,KAWAMURA M,KUMAKURA S,et al. Liquid crystal textural analysis based on histogram homogeneity and peak detection algorithm[J]. Liquid crystals,2012,39(4):415-418.
[10]方晶璐,牛萍娟,田会娟.电流对LED特性参数的影响[J].电工技术学报,2013,28(z2):234-238.FANG Jinglu,NIU Pingjuan,TIAN Huijuan. The influence on characteristics parameters for white-LED with current[J].Transactions of China electrotechnical society, 2013, 28(S2):234-238.
[11]崔泽英,谷青博.LED光源光衰规律研究[J].半导体技术,2012,37(4):312-315.CUI Zeying,GU Qingbo. Research on LED light source lumens depreciation rule[J]. Semiconductor technology,2012,37(4):312-315.