闭锁蛋白和闭锁小带蛋白在慢性胃炎组织中的表达和意义
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摘要
背景:慢性胃炎发病的过程中,生物因子的改变可影响胃黏膜的生理屏障,增加细胞间连接的损伤风险,导致黏膜上皮屏障破坏。目的:探讨闭锁蛋白(occludin)、闭锁小带蛋白-1(ZO-1)在慢性胃炎组织中的表达和意义。方法:选取2014年10月—2017年10月于孝昌县第一人民医院就诊的慢性胃炎患者82例,根据内镜活检病理结果分为慢性非萎缩性胃炎(CNAG)组和慢性萎缩性胃炎(CAG)组,选取同期健康志愿者40名作为正常对照组。采用免疫组化方法检测胃黏膜组织occludin、ZO-1表达。幽门螺杆菌(Hp)阳性患者给予Hp根除治疗,转阴者复查occludin、ZO-1表达。结果:CNAG、CAG组occludin、ZO-1蛋白阳性表达率显著低于正常对照组(P <0. 05); CNAG组occludin、ZO-1蛋白阳性表达率显著高于CAG组(P <0. 05)。CNAG Hp阳性组occludin、ZO-1蛋白阳性表达率显著低于Hp阴性组(P <0. 05); CAG Hp阳性组ZO-1蛋白阳性表达率显著低于Hp阴性组(P <0. 05)。Occludin、ZO-1蛋白表达与胃黏膜炎症活动度呈线性趋势关系(P <0. 05),随着炎症活动度的增加,两者表达显著减弱。CNAG Hp阳性患者转阴后,occludin、ZO-1蛋白阳性表达率较根除治疗前显著升高(P <0. 05)。结论:Occludin、ZO-1在慢性胃炎组织尤其是CAG中表达减弱。两者表达与Hp感染以及胃炎活动度之间存在一定联系。
Background: In the pathogenesis of chronic gastritis,the changes of biological factors can affect the physiological barrier of gastric mucosa,increase the risk of injury of intercellular junctions,leading to disruption of mucosal barrier.Aims: To investigate the expressions of occludin and zonula occluden-1 (ZO-1) in chronic gastritis and their significance.Methods: A total of 82 patients with chronic gastritis from October 2014 to October 2017 at the First People's Hospital of Xiaochang were enrolled and divided into chronic non-atrophic gastritis (CNAG) group and chronic atrophic gastritis (CAG) group according to the endoscopic biopsy pathology. Forty healthy subjects were served as normal controls.Expressions of occludin and ZO-1 were examined by immunohistochemistry. Patients positive for Helicobacter pylori (Hp)infection received eradication therapy,and expressions of occludin and ZO-1 were reexamined after Hp eradication.Results: The positivity rates of occludin and ZO-1 proteins in CNAG and CAG groups were significantly lower than those in normal control group (P < 0. 05). The positivity rates of occludin and ZO-1 proteins in CNAG group were significantly higher than those in CAG group (P < 0. 05). The positivity rates of occludin and ZO-1 proteins in Hp-positive CNAG patients were significantly lower than those in Hp-negative CNAG patients (P < 0. 05). The positivity rate of ZO-1 protein in Hp-positive CAG patients was significantly lower than that in Hp-negative CAG patients (P < 0. 05). Expressions of occludin and ZO-1 proteins showed a linear trend correlation with activity of gastric inflammation (P < 0. 05); their expressions decreased significantly with the increase in activity of inflammation. The positivity rates of occludin and ZO-1 proteins in CNAG patients increased significantly after Hp eradication (P < 0. 05). Conclusions: Expressions of occludin and ZO-1 are decreased in chronic gastritis,especially in CAG,and are correlated with Hp infection and activity of gastric inflammation.
Background: In the pathogenesis of chronic gastritis,the changes of biological factors can affect the physiological barrier of gastric mucosa,increase the risk of injury of intercellular junctions,leading to disruption of mucosal barrier.Aims: To investigate the expressions of occludin and zonula occluden-1 (ZO-1) in chronic gastritis and their significance.Methods: A total of 82 patients with chronic gastritis from October 2014 to October 2017 at the First People's Hospital of Xiaochang were enrolled and divided into chronic non-atrophic gastritis (CNAG) group and chronic atrophic gastritis (CAG) group according to the endoscopic biopsy pathology. Forty healthy subjects were served as normal controls.Expressions of occludin and ZO-1 were examined by immunohistochemistry. Patients positive for Helicobacter pylori (Hp)infection received eradication therapy,and expressions of occludin and ZO-1 were reexamined after Hp eradication.Results: The positivity rates of occludin and ZO-1 proteins in CNAG and CAG groups were significantly lower than those in normal control group (P < 0. 05). The positivity rates of occludin and ZO-1 proteins in CNAG group were significantly higher than those in CAG group (P < 0. 05). The positivity rates of occludin and ZO-1 proteins in Hp-positive CNAG patients were significantly lower than those in Hp-negative CNAG patients (P < 0. 05). The positivity rate of ZO-1 protein in Hp-positive CAG patients was significantly lower than that in Hp-negative CAG patients (P < 0. 05). Expressions of occludin and ZO-1 proteins showed a linear trend correlation with activity of gastric inflammation (P < 0. 05); their expressions decreased significantly with the increase in activity of inflammation. The positivity rates of occludin and ZO-1 proteins in CNAG patients increased significantly after Hp eradication (P < 0. 05). Conclusions: Expressions of occludin and ZO-1 are decreased in chronic gastritis,especially in CAG,and are correlated with Hp infection and activity of gastric inflammation.
引文
1邵立健,朱清仙.一种跨膜蛋白---闭锁蛋白的研究现状[J].国外医学·生理、病理科学与临床分册,2004,24(3):263-266.
2中华医学会消化病学分会.中国慢性胃炎共识意见(上)(2006,上海)[J].中华消化杂志,2007,27(1):45-49.
3 Wroblewski LE,Shen L,Ogden S,et al.Helicobacter pylori dysregulation of gastric epithelial tight junctions by urease-mediated myosinⅡactivation[J].Gastroenterology,2009,136(1):236-246.
4 Amieva MR,Vogelmann R,Covacci A,et al.Disruption of the epithelial apical-junctional complex by Helicobacter pylori Cag A[J].Science,2003,300(5624):1430-1434.
5樊叶,汪志兵,关月,等.紧密连接蛋白Occludin及ZO-1在根除幽门螺杆菌慢性胃炎组织中的表达[J].胃肠病学和肝病学杂志,2017,26(4):440-443.
2中华医学会消化病学分会.中国慢性胃炎共识意见(上)(2006,上海)[J].中华消化杂志,2007,27(1):45-49.
3 Wroblewski LE,Shen L,Ogden S,et al.Helicobacter pylori dysregulation of gastric epithelial tight junctions by urease-mediated myosinⅡactivation[J].Gastroenterology,2009,136(1):236-246.
4 Amieva MR,Vogelmann R,Covacci A,et al.Disruption of the epithelial apical-junctional complex by Helicobacter pylori Cag A[J].Science,2003,300(5624):1430-1434.
5樊叶,汪志兵,关月,等.紧密连接蛋白Occludin及ZO-1在根除幽门螺杆菌慢性胃炎组织中的表达[J].胃肠病学和肝病学杂志,2017,26(4):440-443.