民用航空器物体表面微生物污染现状调查
|
摘要
目的了解民用航空器物体表面的微生物污染状况和分布规律,为航空器的卫生消毒提供参考。方法采用现场采样和细菌分离培养方法,对国内3家航空公司不同航空器高频接触物体表面进行采样和细菌培养,并进行病原菌鉴定。结果共采集不同物体表面标本997件,以机舱卫生间马桶圈和马桶座检出菌数最高,分别为20 cfu/件和30 cfu/件;卫生间内扶手和洗手池检出菌数均为10 cfu/件;机舱内小桌板和座椅扶手检出菌数均<2 cfu/cm~2。共分离出55种细菌,马桶座上检出的细菌93. 8%为致病菌和条件致病菌。结论民用航空器高频接触物体表面污染不容忽视,远航程飞机舱内、舱内卫生间等污染更严重,建议对航空器开展致病菌监测和消毒工作。
Objective To investigate the microbial contamination and the regularities of distribution on aircraft cabin surface,provide reference for developing disinfection and control strategies on aircraft. Methods Field sampling and bacterial isolation and cultivation methods were used to detect the bacterium carried by highly touched objects and then identify.Results 997 samples of surface were monitored. The highest bacterial count detected out was on toilet lid and seat ring,20 cfu/item and 30 cfu/item respectively. The bacterial count of handrail and hand washing sink in toilet were 10 cfu/item.The bacterial count of. traytables and armrests were under 2 cfu/cm~2. 55 strains of clinical or opportunistic pathogenic were isolated,93. 8% of them were primarily from surface of toilet seat. Conclusion The microbial contamination of toilet were serious,especially in the cabin and toilet of long distance airplanes. The pathogen surveillance and disinfection should be done regularity in aircraft cabin.
Objective To investigate the microbial contamination and the regularities of distribution on aircraft cabin surface,provide reference for developing disinfection and control strategies on aircraft. Methods Field sampling and bacterial isolation and cultivation methods were used to detect the bacterium carried by highly touched objects and then identify.Results 997 samples of surface were monitored. The highest bacterial count detected out was on toilet lid and seat ring,20 cfu/item and 30 cfu/item respectively. The bacterial count of handrail and hand washing sink in toilet were 10 cfu/item.The bacterial count of. traytables and armrests were under 2 cfu/cm~2. 55 strains of clinical or opportunistic pathogenic were isolated,93. 8% of them were primarily from surface of toilet seat. Conclusion The microbial contamination of toilet were serious,especially in the cabin and toilet of long distance airplanes. The pathogen surveillance and disinfection should be done regularity in aircraft cabin.
引文
[1]叶应妩,王毓三,申子瑜,等.全国临床检验操作规范[M].第3版.江苏南京:东南大学出版社,2006:736-753.
[2]Michael GS,Hubert HA,Peter AS,et al.Sustained reduction of microbial burden on common hospital surfaces through introduction of copper[J].J Clin Microbiol,2012,50(7):2217-2223.
[3]Wagenv JH,Sluijsmans W,Penders RJ.Better environmental survival of outbreak vs.sporadic MRSA isolates[J].Hosp Infect,2000,45:231-234.
[4]Thornley CN,Emslie NA,Sprott TW,et al.Recurring norovirus transmission on an airplane[J].Clin Infect Dis,2011,53(6):521-526.
[5]中华人民共和国卫生部.GB/T 15982-2012医院消毒卫生标准[S].2012.
[6]World Health Organization.Guide to hygiene and sanitation in aviation[M].3rd.Geneva,WHO,2009:31-38.
[7]World Health Organization.International Health Regulations(2005)[M].Geneva:World Health Organization,2005.
[8]张亮.动车组列车公共设施及公共用品微生物污染状况调查[J].现代预防医学,2016,43(10):1901-1902.
[9]Report to the FAA on the Airliner Cabin Environment Report No.RITE-CoE-2010-1[R].2010:87-96.
[10]Rusin P,Maxwell S,Gerba C.Comparative surface-to-hand and fingertip-to-mouth transfer efficiency of gram-positive bacteria,gram-negative bacteria,and phage[J].J Appl Microbiol,2002,93(4):585-592.
[11]Nordahl TP,Rasmussen S,Hasman H,et al.Meta-genomic analysis of toilet waste from long distance flights;a step towards global surveillance of infectious diseases and antimicrobial resistance[J].Sci Rep,2015,5(1):1-9.
[12]范锦辉,周毓瑾,魏秋华,等.民用航空器客舱物体表面消毒效果评价[J].中国消毒学杂志,2017,34(12):1141-1116.
[13]班海群,朱仁义,刘晓杰,等.医院物体表面微生物污染现状及控制策略研究[J].中国消毒学杂志,2015,32(7):649-653.
[2]Michael GS,Hubert HA,Peter AS,et al.Sustained reduction of microbial burden on common hospital surfaces through introduction of copper[J].J Clin Microbiol,2012,50(7):2217-2223.
[3]Wagenv JH,Sluijsmans W,Penders RJ.Better environmental survival of outbreak vs.sporadic MRSA isolates[J].Hosp Infect,2000,45:231-234.
[4]Thornley CN,Emslie NA,Sprott TW,et al.Recurring norovirus transmission on an airplane[J].Clin Infect Dis,2011,53(6):521-526.
[5]中华人民共和国卫生部.GB/T 15982-2012医院消毒卫生标准[S].2012.
[6]World Health Organization.Guide to hygiene and sanitation in aviation[M].3rd.Geneva,WHO,2009:31-38.
[7]World Health Organization.International Health Regulations(2005)[M].Geneva:World Health Organization,2005.
[8]张亮.动车组列车公共设施及公共用品微生物污染状况调查[J].现代预防医学,2016,43(10):1901-1902.
[9]Report to the FAA on the Airliner Cabin Environment Report No.RITE-CoE-2010-1[R].2010:87-96.
[10]Rusin P,Maxwell S,Gerba C.Comparative surface-to-hand and fingertip-to-mouth transfer efficiency of gram-positive bacteria,gram-negative bacteria,and phage[J].J Appl Microbiol,2002,93(4):585-592.
[11]Nordahl TP,Rasmussen S,Hasman H,et al.Meta-genomic analysis of toilet waste from long distance flights;a step towards global surveillance of infectious diseases and antimicrobial resistance[J].Sci Rep,2015,5(1):1-9.
[12]范锦辉,周毓瑾,魏秋华,等.民用航空器客舱物体表面消毒效果评价[J].中国消毒学杂志,2017,34(12):1141-1116.
[13]班海群,朱仁义,刘晓杰,等.医院物体表面微生物污染现状及控制策略研究[J].中国消毒学杂志,2015,32(7):649-653.