慢性汞中毒对肾小球血管内皮细胞的影响及机制研究
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摘要
目的探讨慢性汞中毒对肾小球内皮细胞的影响及作用机制。方法采用SD大鼠建立慢性汞中毒模型,HE、PAS观察肾脏病理变化;氯化汞刺激人肾小球内皮细胞观察对内皮细胞的影响及相关蛋白表达。结果慢性汞中毒可致肾小管、肾小球广泛变性坏死,内皮细胞运动、迁移能力减弱,差异具有统计学意义(P<0.01),相关蛋白表达异常,并有剂量依赖性。结论根据汞致肾损伤的变化为法医诊断慢性汞中毒提供理论基础。
Objective To investigate the effect of chronic mercury poisoning on glomerular endothelial cell injury and its mechanism. Methods SD rats were used to establish chronic mercury poisoning model HE and PAS to observe the pathological changes of the kidney. Mercury stimulated human glomerular endothelial cells to observe the effect on endothelial cells and the expression of related proteins. Results Chronic mercury poisoning caused extensive degeneration and necrosis of renal tubules and glomeruli. The endothelial cell movement and migration ability were weakened, and the difference was statistically significant(P<0.01). The expression of related proteins was abnormal and dose-dependent. Conclusion Provide a theoretical basis for pathological forensic diagnosis of chronic mercury poisoning.
Objective To investigate the effect of chronic mercury poisoning on glomerular endothelial cell injury and its mechanism. Methods SD rats were used to establish chronic mercury poisoning model HE and PAS to observe the pathological changes of the kidney. Mercury stimulated human glomerular endothelial cells to observe the effect on endothelial cells and the expression of related proteins. Results Chronic mercury poisoning caused extensive degeneration and necrosis of renal tubules and glomeruli. The endothelial cell movement and migration ability were weakened, and the difference was statistically significant(P<0.01). The expression of related proteins was abnormal and dose-dependent. Conclusion Provide a theoretical basis for pathological forensic diagnosis of chronic mercury poisoning.
引文
[1]李美雄,谭灶芹,刘宇.以疼痛为表现的慢性汞中毒的临床观察分析[J].中国冶金工业医学杂志,2011,28(5):616-617.
[2]Clarkson TW,Magos L.The toxicology of mercury--current exposures and clinical manifestations[J].N Engl JMed,2003,349(18):1731-1737.
[3]Wiggers GA,Furieri LB,Briones AM,et al.Cerebrovascular endothelial dysfunction induced by mercury exposureatlow con centrations[J].Neurotoxicology,2016,53:282-289.
[4]王汉斌,肖李帅,熊锡山.不同接触途径致汞中毒诊断中应注意的问题[J].中国医刊,2012(2):3-5.
[5]Nath KA,Croatf AJ,Likely S,et al.Renal oxidant injury and oxidant response induced by mercury[J].Kidney Lnt,1996,50(3):1032.
[6]Hua J,Pelleter,Berlin M,et al.Autoimmune glomerulon-ephritis induced by mercury vapour ex posure in the brown norway rat[J].Toxicology,1993,79:119.
[7]Abedi VM,Hu H,Moller G.Mercury induced venal immune complex deposits in young mice[J].Clin Exp Immunol,1997,110(1):86.
[8]肖志朋,李美华.血管内皮钙粘蛋白相关信号及其调控的研究进展[J].广东医学,2015,36(16):2590-2593.
[9]黄健,刘畅,陈松,等.Wnt/β-catenin信号通路在肾缺血再灌注大鼠中的表达及ICG-001阻断对慢性肾间质纤维化的作用[J].实用医学杂志,2018,34(7):1085-1088.
[10]邢玉洁,张荣怀,吕颖,等.Wnt/β-catenin信号通路在硒缺乏大鼠心力衰竭中的调控作用[J/OL].山西医科大学学报,2018,49(9):1003-1006.
[2]Clarkson TW,Magos L.The toxicology of mercury--current exposures and clinical manifestations[J].N Engl JMed,2003,349(18):1731-1737.
[3]Wiggers GA,Furieri LB,Briones AM,et al.Cerebrovascular endothelial dysfunction induced by mercury exposureatlow con centrations[J].Neurotoxicology,2016,53:282-289.
[4]王汉斌,肖李帅,熊锡山.不同接触途径致汞中毒诊断中应注意的问题[J].中国医刊,2012(2):3-5.
[5]Nath KA,Croatf AJ,Likely S,et al.Renal oxidant injury and oxidant response induced by mercury[J].Kidney Lnt,1996,50(3):1032.
[6]Hua J,Pelleter,Berlin M,et al.Autoimmune glomerulon-ephritis induced by mercury vapour ex posure in the brown norway rat[J].Toxicology,1993,79:119.
[7]Abedi VM,Hu H,Moller G.Mercury induced venal immune complex deposits in young mice[J].Clin Exp Immunol,1997,110(1):86.
[8]肖志朋,李美华.血管内皮钙粘蛋白相关信号及其调控的研究进展[J].广东医学,2015,36(16):2590-2593.
[9]黄健,刘畅,陈松,等.Wnt/β-catenin信号通路在肾缺血再灌注大鼠中的表达及ICG-001阻断对慢性肾间质纤维化的作用[J].实用医学杂志,2018,34(7):1085-1088.
[10]邢玉洁,张荣怀,吕颖,等.Wnt/β-catenin信号通路在硒缺乏大鼠心力衰竭中的调控作用[J/OL].山西医科大学学报,2018,49(9):1003-1006.