聪明方对老年痴呆模型大鼠行为学能力及α-β-分泌酶的影响
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摘要
目的观察聪明方对老年痴呆模型大鼠行为学能力及α-β-分泌酶的影响。方法以腹腔注射D-半乳糖+双侧海马CA1区一次性注射聚集态Aβ1-40复合造模方法制作老年痴呆大鼠模型,通过Morris水迷宫实验系统测试模型动物的行为学能力,提取并测定大鼠海马α-β-分泌酶,采用SPSS13.0软件进行统计学处理。结果聪明方能缩短老年痴呆模型大鼠在Morris水迷宫测试中的逃避潜伏期,延长原平台区停留时间和增加经过平台的次数(P<0.05),且聪明方低、中、高剂量组α-分泌酶的MOD均明显升高,β-分泌酶的MOD均明显降低,且与低剂量组比较,中、高剂量组α-分泌酶的MOD明显升高,β-分泌酶的MOD均明显降低(P<0.05,或P<0.01)。结论聪明方能有效改善老年痴呆模型大鼠行为学能力,并能有效上调α-分泌酶,抑制β-分泌酶。
Objective To observe the effect of clever prescription on behavioral ability and alpha-beta-secretase in senile dementia rats. Methods Alzheimer's disease rat model was established by intraperitoneal injection of D-galactose plus one-time injection of aggregated A-beta 1-40 into CA1 area of bilateral hippocampus, Morris water maze system was used to test the behavioral ability of the model rats, and the hippocampal alpha-beta-secretase was extracted and measured, SPSS13.0 software was used for statistical analysis. Results Smart prescription could shorten the escape latency, prolong the residence time in the original platform area and increase the number of times passing through the platform(P<0.05) Compared with the control group, the MOD of alpha-secretase was significantly increased and that of beta-secretase was significantly decreased(P<0.05 or P<0.01). Conclusion The clever prescription can effectively improve the behavioral ability of senile dementia rats, and can effectively up-regulate alpha-secretase and inhibit beta-secretase.
Objective To observe the effect of clever prescription on behavioral ability and alpha-beta-secretase in senile dementia rats. Methods Alzheimer's disease rat model was established by intraperitoneal injection of D-galactose plus one-time injection of aggregated A-beta 1-40 into CA1 area of bilateral hippocampus, Morris water maze system was used to test the behavioral ability of the model rats, and the hippocampal alpha-beta-secretase was extracted and measured, SPSS13.0 software was used for statistical analysis. Results Smart prescription could shorten the escape latency, prolong the residence time in the original platform area and increase the number of times passing through the platform(P<0.05) Compared with the control group, the MOD of alpha-secretase was significantly increased and that of beta-secretase was significantly decreased(P<0.05 or P<0.01). Conclusion The clever prescription can effectively improve the behavioral ability of senile dementia rats, and can effectively up-regulate alpha-secretase and inhibit beta-secretase.
引文
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[2]陈新平,陈彪.阿尔茨海默病病因学及发病机制研究进展[J].中国现代神经疾病杂志,2005,5(3):152-155.
[3]刘娟,杨立,张洪.中医药治疗阿尔兹海默病的研究进展[J].世界中医药,2016,11(5):932-935.
[4]李春花,高爱社.中医药治疗阿尔茨海默病的研究进展[J].中医临床研究,2017(28):139-141.
[5]张景富.中医药治疗阿尔茨海默病研究进展[J].中国药业,2015(b11):363-364.
[6]周娇娇,阙建宇,于雯雯,等.Morris水迷宫检测动物学习记忆水平的方法学[J].中国老年学杂志,2017,37(24):6274-6277.
[7]武海霞,吴志刚,刘红彬,等.Morris水迷宫实验在空间学习记忆研究中的应用[J].神经药理学报,2014,4(5):30-35.
[8]徐方剑,王逸文,陆寒烨,等.低功率密度微波辐射对小鼠学习记忆的影响[J].环境与职业医学,2017,34(1):76-78.
[9]李妍,朱慧杰,唐盼盼,等.七氟烷对小鼠空间学习与记忆能力的影响[J].中国保健营养,2016,26(17):180-181.
[10]H2.ARDY J A,HIGGINS G A.Alzheimer′s diseases:the amyloid cascade hypothesis[J].Science,1992,256(5054):184-185.
[11]张晓燕,张璐,于莹.阿尔兹海默症动物模型的研究进展[J].实验动物科学,2016,33(5):68-70.
[12]田甜.探讨阿尔茨海默症的诊疗研究进展[J].世界最新医学信息文摘,2015,15(67):29,59.