聚乙二醇渗透处理对水曲柳种子在不同温度下萌发的影响
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摘要
为探究聚乙二醇(PEG)渗透处理对水曲柳种子次生休眠诱导的影响,以解除初生休眠的水曲柳种子为材料,研究经不同质量分数(0,5%,25%,50%)PEG渗透处理不同时间(3,7,14 d)后种子在不同温度(15和25℃)下的萌发表现。结果表明:在适宜萌发温度(15℃)下,PEG渗透处理不会诱导种子进入低水势引起的次生休眠。但不同渗透处理对种子发芽的影响不同:低质量分数(5%)渗透处理和中等质量分数(25%)的短时间(3 d)渗透处理对种子萌发有明显的促进作用,但中等质量质量分数的中等(7 d)和长时间(14 d)渗透处理以及高质量分数(50%)的渗透处理都对种子萌发不利。在高温(25℃)下,中高质量分数PEG短时间渗透处理或高质量分数PEG中长时间渗透处理对种子热休眠诱导有明显的减弱作用,但低质量分数PEG渗透处理没有减弱热休眠的效应。
In order to investigate the effect of polyethylene glycol(PEG) osmotic treatment on secondary dormancy induction of Manchurian ash(Fraxinus mandshurica) seed, the primary dormancy released seeds were used to study the germination performance of seeds at different temperatures(15 ℃ and 25 ℃) after PEG treatment in different concentrations(0%, 5%, 25%, and 50%) for different time(3, 7, and 14 d). The PEG treatment did not induce secondary dormancy caused by low water potential at suitable germination temperature(15 ℃). However, different osmotic treatments have different effects on seed germination: low concentration(5%) treatment and medium concentration(25%) short-time(3 d) treatment have a significant effect on seed germination, but medium concentration moderate(7 d) and long-term(14 d) treatment, and high concentration(50%) treatment are detrimental to seed germination. Under high temperature(25 ℃), the medium to high concentration PEG short-time treatment and moderate to long-term treatment in high concentration significantly circumvent the thermodormancy induction, but the low concentration PEG treatment do not have the effect of circumventing thermodormancy.
In order to investigate the effect of polyethylene glycol(PEG) osmotic treatment on secondary dormancy induction of Manchurian ash(Fraxinus mandshurica) seed, the primary dormancy released seeds were used to study the germination performance of seeds at different temperatures(15 ℃ and 25 ℃) after PEG treatment in different concentrations(0%, 5%, 25%, and 50%) for different time(3, 7, and 14 d). The PEG treatment did not induce secondary dormancy caused by low water potential at suitable germination temperature(15 ℃). However, different osmotic treatments have different effects on seed germination: low concentration(5%) treatment and medium concentration(25%) short-time(3 d) treatment have a significant effect on seed germination, but medium concentration moderate(7 d) and long-term(14 d) treatment, and high concentration(50%) treatment are detrimental to seed germination. Under high temperature(25 ℃), the medium to high concentration PEG short-time treatment and moderate to long-term treatment in high concentration significantly circumvent the thermodormancy induction, but the low concentration PEG treatment do not have the effect of circumventing thermodormancy.
引文
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[18] 王靖靖,余玲,朱恭,等.几种环境因子对黄花矶松种子萌发的影响[J].草业科学,2018,35(7):1661-1669.
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[22] 蔡年辉,许玉兰,张瑞丽,等.云南松种子萌发及芽苗生长对干旱胁迫的响应[J].种子,2012,31(7):44-46.
[23] 郭晋梅,刘娟,董宽虎.PEG胁迫对白羊草种子萌发的影响[J].中国草地学报,2015,37(2):58-62.
[24] CHEN K,ARORA R,ARORA U.Osmopriming of spinach (Spinacia oleracea L.cv.Bloomsdale) seeds and germination performance under temperature and water stress.[J].Seed Science & Technology,2010,38(1):45-57.
[25] 段慧荣,李毅,马彦军.PEG胁迫对沙冬青种子萌发过程的影响[J].水土保持研究,2011,18(3):221-225.
[26] 孙景宽,文辉,张洁明,等.种子萌发期4种植物对干旱胁迫的响应及其抗旱性评价研究[J].西北植物学报,2006,26(9):1811-1818.
[27] 王文斌.植物活性氧代谢及其利用[M].北京:中国农业科学技术出版社,2011:114-150.
[2] HILHOR S T,HENK W M.The regulation of secondary dormancy the membrane hypothesis revisite[J].Seed Science Research,1998,8(2):77-90.
[3] 张鹏.种子休眠相关概念及分类研究进展[J].种子,2012,31(7):54-57,61.
[4] 张鹏,沈海龙.水曲柳种子次生休眠的预防和解除[J].植物生理学通讯,2008,44(6):1149-1151.
[5] MICHEL B E,KAUFMANN M R.The osmotic potential of polyethylene glycol 6000[J].Plant Physiology,1973,51(5):914-916.
[6] 刘佳,徐昌旭,曹卫东,等.PEG胁迫下15份紫云英种质材料萌发期的抗旱性鉴定[J].中国草地学报,2012,34(6):18-25.
[7] 李志萍,张文辉,崔豫川.PEG模拟干旱胁迫对栓皮栎种子萌发及生长生理的影响[J].西北植物学报,2013,33(10):2043-2049.
[8] 闫兴富,周立彪,思彬彬,等.不同温度下PEG-6000模拟干旱对柠条锦鸡儿种子萌发的胁迫效应[J].生态学报,2016,36(7):1989-1996.
[9] CANTLIFFE D J,FISCHER J M,NELL T A.Mechanism of seed priming in circumventing thermodormancy in Lettuce[J].Plant Physiology,1984,75(2):290-294.
[10] GUEDES A C,CANTLIFFE D J,SHULER K D,et al.Overcoming thermodormancy in lettuce by seed priming[J].Proceedings of the Florida State Horticultural Society.1979,92:130-133.
[11] 刘彦文,李明,姚东伟.引发对莴苣种子萌发及减轻其高温休眠的影响[J].种子,2010,29(10):54-56.
[12] BRITO C D D,LOUREIRO M B,RIBEIRO P R,et al.Osmoconditioning prevents the onset of microtubular cytoskeleton and activation of cell cycle and it is detrimental for germination of Jatropha curcas L.seeds[J].Plant Biology,2016,18(6):1053-1057.
[13] MOMOH E J,ZHOU W J,KRISTIANSSON B.Variation in the development of secondary dormancy in oilseed rape genotypes under conditions of stress[J].Weed Research,2002,42(6):446-455.
[14] BEWLEY J D.Seed germination and dormancy[J].Plant Cell,1997,9(7):1055-1066.
[15] KARSSEN C M,VEGES R.Osmoconditioning of luttuce seeds and induction of secondary dormancy[J].Acta Horticulture,1988,215:165-171.DOI:10.17660/Acta Hortic,1988.221.46.
[16] OBANOR F O,WALTER M,JONES E E,et al.Effect of temperature,relative humidity,leaf wetness and leaf age on Spilocaea oleagina conidium germination on olive leaves[J].European Journal of Plant Pathology,2008,120(3):211-222.
[17] 曾彦军,王彦荣,保平,等.几种生态因子对红砂和霸王种子萌发与幼苗生长的影响[J].草业学报,2005,14(5):24-31.
[18] 王靖靖,余玲,朱恭,等.几种环境因子对黄花矶松种子萌发的影响[J].草业科学,2018,35(7):1661-1669.
[19] PEKRUN C,LUTMAN P J W,BAEUMER K.Induction of secondary dormancy in rape seeds (Brassica napus L.) by prolonged imbibition under conditions of water stress or oxygen deficiency in darkness[J].European Journal of Agronomy,1997,6(3/4):245-255.
[20] SOLTANI E,GRUBER S,OVEISI M,et al.Water stress,temperature regimes and light control induction,and loss of secondary dormancy in Brassica napus L.seeds[J].Seed Science Research,2017,27(3):217-230.
[21] 吴敏,张文辉,马闯,等.干旱胁迫对栓皮栎种子萌发能力的影响[J].西北农林科技大学学报(自然科学版),2017,45(5):91-100.
[22] 蔡年辉,许玉兰,张瑞丽,等.云南松种子萌发及芽苗生长对干旱胁迫的响应[J].种子,2012,31(7):44-46.
[23] 郭晋梅,刘娟,董宽虎.PEG胁迫对白羊草种子萌发的影响[J].中国草地学报,2015,37(2):58-62.
[24] CHEN K,ARORA R,ARORA U.Osmopriming of spinach (Spinacia oleracea L.cv.Bloomsdale) seeds and germination performance under temperature and water stress.[J].Seed Science & Technology,2010,38(1):45-57.
[25] 段慧荣,李毅,马彦军.PEG胁迫对沙冬青种子萌发过程的影响[J].水土保持研究,2011,18(3):221-225.
[26] 孙景宽,文辉,张洁明,等.种子萌发期4种植物对干旱胁迫的响应及其抗旱性评价研究[J].西北植物学报,2006,26(9):1811-1818.
[27] 王文斌.植物活性氧代谢及其利用[M].北京:中国农业科学技术出版社,2011:114-150.