绞股蓝黄芪保健茶对免疫功能的影响
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摘要
研究绞股蓝黄芪保健茶对小鼠免疫功能的影响。将200只清洁级ICR雌性小鼠,按体重分成免疫5大组,每组随机分成4小组,每小组10只;按人日推荐用量的5倍、10倍、30倍,分别设0.415、0.830、2.490g/kg-Bw 3个剂量组和蒸馏水对照组。小鼠每天按20mL/kg BW连续灌胃30d后,分别测定小鼠的体重、脏器指数、细胞免疫功能、体液免疫功能、单核-巨噬功能以及NK细胞活性。结果表明:样品高剂量组小鼠淋转OD差值、抗体积数、溶血空斑数和碳廓清吞噬指数a都明显高于对照组,经统计,差异均有统计学意义(p<0.05);高剂量的样品对小鼠的免疫功能有明显的增强作用。
To study the effect of gynostemma astragalus health tea on immune function in mice. 200 clean grade ICR female mice were divided into 5 groups according to their body weight. Each group was randomly divided into 4 groups with 10 in each group. According to the recommended daily dosage of 5 times,10 times,and 30 times,0.415,0.830 and 2.490 g/kg Bw were set with distilled water control group. The mice were continuously fed with 20 mL/kg BW daily for 30 days. The body weight,organ index,cellular immune function,humoral immune function,monocyte-macrophage function and NK cell activity were measured. The results showed that the OD value,anti-volume number,hemolytic plaque number,and carbon clearance phagocytic index a of the high-dose mice in the high-dose group were significantly higher than those in the control group. Statistically,the differences were statistically significant(p<0.05). High-dose samples have a significant enhancement of immune function in mice.
To study the effect of gynostemma astragalus health tea on immune function in mice. 200 clean grade ICR female mice were divided into 5 groups according to their body weight. Each group was randomly divided into 4 groups with 10 in each group. According to the recommended daily dosage of 5 times,10 times,and 30 times,0.415,0.830 and 2.490 g/kg Bw were set with distilled water control group. The mice were continuously fed with 20 mL/kg BW daily for 30 days. The body weight,organ index,cellular immune function,humoral immune function,monocyte-macrophage function and NK cell activity were measured. The results showed that the OD value,anti-volume number,hemolytic plaque number,and carbon clearance phagocytic index a of the high-dose mice in the high-dose group were significantly higher than those in the control group. Statistically,the differences were statistically significant(p<0.05). High-dose samples have a significant enhancement of immune function in mice.
引文
[1]张海燕,郭强,沤伟业.绞股蓝总皂甙对小鼠免疫功能的影响[J].中兽医学杂志,2006(2):13-15.
[2]张崇泉,燕美玉,杨晓慧,等.绞股蓝总皂甙免疫调节作用的研究[J].中西医结合杂志,1990,10(2):96-98.
[3]李艳茹.绞股蓝多糖对疲劳运动小鼠免疫能力的影响[J].食品科学,2008,29(08):584-586.
[4]钱新华,王佾先,唐晓玲,等.绞股蓝多糖对免疫功能的影响[J].中国医科大学学报,1998,30(1):51-53.
[5]唐晓玲,郑肇巽,夏培源.绞股蓝多糖对小鼠T淋巴细胞酯酶染色率的影响[J].江苏药学与临床研究,1996,4(1):15-16.
[6]刘印华,赵志强,李树义,等.黄芪多糖对免疫功能影响的体内实验研究[J].河北医药,2015,37(4):485-487.
[7]卢炜,顾蓓蓓,卢劲晔,等.黄芪多糖对小鼠免疫功能影响的研究[J].兽药应用,2014,30(11):202-203.
[8]李天一,汪丽佩,吴国琳.黄芪多糖对免疫性肝损伤小鼠免疫调节的影响[J].中国中医急症,2014,23(1):25-27.
[9]黄志远,黄家涛.黄芪多糖对气虚小鼠免疫功能的作用研究[J].中国当代医药,2014,21(20):35-37.
[10]张建伟,刘海燕,张淼.茶多酚对大强度耐力运动小鼠免疫功能的影响[J].食品工业科技,2012,33(11):367-369.
[11]蔡海莹,张伟力,朱建和,等.茶多酚对生长猪机体免疫力的影响[J].饲料研究,2006(6):55-56.
[12]沈健,陈增良,鲍建芳,等.茶多糖对实验性免疫力低下小鼠免疫功能的影响[J].浙江预防医学,2007,19(4):96-97.
[13]中华人民共和国卫生部.保健食品检验与评价技术规范[S].中华人民共和国卫生部,2003,22-33.
[14]郭蔼春.黄帝内经素问校注语译[M].贵阳:贵州教育出版社,2010:88-89.
[15]张钊,李鹏婧,杨洋等.绞股蓝研究综述[J].食品研究与开发,2011,32(11):193-196.
[16]张国用.中药黄芪的药理作用及其临床应用研究[J].实用心脑肺血管病杂志,2012,6(20):1059-1060.
[17]Abul K A,Andrew H L.Basic Immunology Updated Edition:Functions and Disorders of Disorders of the Immune System[M].Philadelphia:Saunders Elsevier,2011:13-16.
[2]张崇泉,燕美玉,杨晓慧,等.绞股蓝总皂甙免疫调节作用的研究[J].中西医结合杂志,1990,10(2):96-98.
[3]李艳茹.绞股蓝多糖对疲劳运动小鼠免疫能力的影响[J].食品科学,2008,29(08):584-586.
[4]钱新华,王佾先,唐晓玲,等.绞股蓝多糖对免疫功能的影响[J].中国医科大学学报,1998,30(1):51-53.
[5]唐晓玲,郑肇巽,夏培源.绞股蓝多糖对小鼠T淋巴细胞酯酶染色率的影响[J].江苏药学与临床研究,1996,4(1):15-16.
[6]刘印华,赵志强,李树义,等.黄芪多糖对免疫功能影响的体内实验研究[J].河北医药,2015,37(4):485-487.
[7]卢炜,顾蓓蓓,卢劲晔,等.黄芪多糖对小鼠免疫功能影响的研究[J].兽药应用,2014,30(11):202-203.
[8]李天一,汪丽佩,吴国琳.黄芪多糖对免疫性肝损伤小鼠免疫调节的影响[J].中国中医急症,2014,23(1):25-27.
[9]黄志远,黄家涛.黄芪多糖对气虚小鼠免疫功能的作用研究[J].中国当代医药,2014,21(20):35-37.
[10]张建伟,刘海燕,张淼.茶多酚对大强度耐力运动小鼠免疫功能的影响[J].食品工业科技,2012,33(11):367-369.
[11]蔡海莹,张伟力,朱建和,等.茶多酚对生长猪机体免疫力的影响[J].饲料研究,2006(6):55-56.
[12]沈健,陈增良,鲍建芳,等.茶多糖对实验性免疫力低下小鼠免疫功能的影响[J].浙江预防医学,2007,19(4):96-97.
[13]中华人民共和国卫生部.保健食品检验与评价技术规范[S].中华人民共和国卫生部,2003,22-33.
[14]郭蔼春.黄帝内经素问校注语译[M].贵阳:贵州教育出版社,2010:88-89.
[15]张钊,李鹏婧,杨洋等.绞股蓝研究综述[J].食品研究与开发,2011,32(11):193-196.
[16]张国用.中药黄芪的药理作用及其临床应用研究[J].实用心脑肺血管病杂志,2012,6(20):1059-1060.
[17]Abul K A,Andrew H L.Basic Immunology Updated Edition:Functions and Disorders of Disorders of the Immune System[M].Philadelphia:Saunders Elsevier,2011:13-16.